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Vet Immunol Immunopathol ; 21(3-4): 261-78, 1989 Jul.
Article in English | MEDLINE | ID: mdl-2678728

ABSTRACT

A sequence encoding bovine granulocyte-macrophage colony-stimulating factor (GM-CSF) has been identified from a concanavalin A-stimulated bovine lymphocyte cDNA library. This sequence was isolated by hybridization with synthetic oligonucleotide probes based upon the human GM-CSF sequence. This bovine cDNA was engineered for expression and secretion of activity into the periplasmic space of E. coli. Periplasmic extracts contain a 14,500-dalton protein and stimulate colony formation of bovine bone marrow progenitor cells. The predicted protein is 70% homologous with human GM-CSF and 55% homologous with murine GM-CSF. Numerous structural features are conserved among these three proteins, such as location of cysteine residues, glycosylation sites, and overall change. The biological activity of bovine GM-CSF is species specific, since recombinant preparations do not cause proliferation of human or murine bone marrow cells. Similarly, murine GM-CSF does not exhibit activity on cells of bovine or human origin. However, human GM-CSF does stimulate colony formation of bovine bone marrow cells, although the specific activity appears reduced when compared to assays on human cells.


Subject(s)
Cattle/genetics , Cloning, Molecular , Colony-Stimulating Factors/genetics , DNA , Growth Substances/genetics , Amino Acid Sequence , Animals , Base Sequence , Cattle/immunology , Colony-Forming Units Assay , Colony-Stimulating Factors/pharmacology , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Gene Library , Granulocyte-Macrophage Colony-Stimulating Factor , Growth Substances/pharmacology , Immunoblotting , Mice , Molecular Sequence Data , Plasmids , Sequence Homology, Nucleic Acid , Species Specificity
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