ABSTRACT
BACKGROUND AND AIMS: Several studies have tried to link the relationship of tissue necrosis factor-alpha (TNF-α) -308 G/A polymorphism with the development of diabetic nephropathy (DN). However, these studies failed to reach a consensus due to conflicting results. This meta-analysis was done to thoroughly investigate the correlation between the polymorphism and DN development. METHODS: To carry out the objective, patients with type 2 diabetes mellitus (T2DM) were used as controls, while patients who developed DN were utilized as cases. Eight studies (i.e., published between 2007 and 2018) were included in the present meta-analysis. Review Manager 5.3 was used to compute for the odds ratios (OR) and 95% confidence interval (CI) of the overall and post-outlier outcomes. RESULTS: Overall, an association between DN development and the -308 G/A polymorphism was observed. However, Galbraith's plot analysis (as analyzed using Meta-Essentials) led to the removal of two studies, which significantly reduced heterogeneity. Post-outlier outcomes show significant results in the allelic (OR: 1.23; 95% CI: 1.01-1.50; p = 0.04) and co-dominant (OR: 1.60; 95% CI: 1.02-2.51; p = 0.04) models. CONCLUSION: T2DM individuals with the -308 G/A polymorphism in the TNF-α gene are more likely to develop DN.
Subject(s)
Diabetes Mellitus, Type 2/complications , Diabetic Nephropathies/pathology , Genetic Predisposition to Disease , Polymorphism, Single Nucleotide , Tumor Necrosis Factor-alpha/genetics , Case-Control Studies , Diabetic Nephropathies/etiology , Diabetic Nephropathies/metabolism , Humans , Prognosis , Risk FactorsABSTRACT
AIMS: Diabetes Mellitus (DM) continues to burden millions of people worldwide. Early detection and effective diagnosis of DM are essential key strategies to reduce the impeding incidence of the disease and its complications. Thus, this study determined the potential utility of salivary glucose, amylase, calcium, and phosphorus as non-invasive diagnostic markers of DM. MATERIALS AND METHODS: A total of 80 participants were recruited and divided into two groups (non-diabetics and diabetics). Fasting blood samples and unstimulated saliva samples were collected and tested for glucose, amylase, calcium, and phosphorus. RESULTS: Mann-Whitney U test shows that salivary glucose and salivary amylase were significantly higher among diabetics than non-diabetics. In addition to this, the receiver operations characteristics (ROC) curve showed that salivary glucose (AUCâ¯=â¯0.811, pâ¯<â¯0.001) and amylase (AUCâ¯=â¯0.649, pâ¯=â¯0.03) has significant association with DM. CONCLUSION: Overall, only salivary glucose and amylase showed good potential in discriminating patients with diabetes from those without.
Subject(s)
Amylases/metabolism , Biomarkers/metabolism , Calcium/metabolism , Diabetes Mellitus, Type 2/diagnosis , Glucose/metabolism , Phosphorus/metabolism , Saliva/metabolism , Adolescent , Adult , Aged , Aged, 80 and over , Case-Control Studies , Cross-Sectional Studies , Diabetes Mellitus, Type 2/metabolism , Female , Follow-Up Studies , Humans , Male , Middle Aged , Prognosis , ROC Curve , Young AdultABSTRACT
OBJECTIVE: The etiology of polycystic ovarian syndrome (PCOS) has not yet been fully explained. Several studies suggested an association between two single nucleotide polymorphisms (T45G and G276T) of the ADIPOQ gene that encodes for the hormone adiponectin and PCOS susceptibility. Hence, we performed a meta-analysis to investigate the relationship of the two further. MATERIALS AND METHODS: Literature search was conducted in PubMed up to June 22, 2018, for related publications written in English. Selected data were extracted from the included studies and was subjected to analysis using Review Manager 5.3. Odds ratios (ORs) and 95% confidence intervals (CIs) were computed and pooled from the resulting studies. Subgroup analysis by ethnicity was also performed. RESULTS: Overall analysis showed that women with the G276T polymorphism have reduced susceptibility to PCOS (OR: 0.68; 95% CI: 0.60-0.78; PA < 0.001). While no significant association was observed for the T45G polymorphism (OR: 1.07; 95% CI: 0.93-1.24; PA = 0.34). Subgroup analysis, on the other hand, showed significant associations among East Asians (OR: 0.69; 95% CI: 0.57-0.82; PA < 0.001) for the G276T association. CONCLUSION: Results of this meta-analysis suggests that women with the G276T polymorphism are less likely to develop PCOS. However, more studies are needed to confirm the claims of this meta-analysis.
