1.
J Chromatogr B Biomed Sci Appl
; 703(1-2): 263-6, 1997 Dec 05.
Article
in English
| MEDLINE
| ID: mdl-9448085
ABSTRACT
Described is a method for the determination of orotic acid as its methyl ester in human urine. The method involves the use of solid-phase extraction to isolate pyrimidines from urine and derivatization with methanol and sulfuric acid, followed by isocratic high-performance liquid chromatography on a reversed-phase C18 column with UV absorbance detection. The assay is shown to be sufficiently sensitive for use in clinical investigations where elevated orotic acid excretion is suspected.