Evaluation of indicators of acute emotional states in dogs.

A complete assessment of animal welfare requires not just an understanding of negative emotional states, such as fear and anxiety, but also of positive states, such as calmness and happiness. However, few studies have identified accurate and reliable indicators of positive emotional states in dogs. This study aimed to identify parameters that may serve as indicators of short-term emotional states in dogs. Using a cross-over design, 60 dogs living at a research facility were exposed to six different 10-min scenarios expected to elicit responses varying in emotional valence and arousal. A range of behavioural and physiological parameters were collected and their relationship to anticipated emotional valence and arousal was analysed using linear and logistic mixed models. Cortisol, adrenocorticotropic hormone, heart rate variability, panting, whining, and body shake all demonstrated significant differences based on arousal levels, but only within negative valence scenarios. Scores from a qualitative behavioural assessment (QBA) were associated with both emotional valence and arousal and were considered the best indicator of positive valence. Activity, ear temperature, and sitting were associated with positive high arousal, although this may have been influenced by differing levels of movement induced during these scenarios. Meanwhile, heart rate, secretory immunoglobulin A, standing and lying all showed similar changes associated with arousal for both positive and negative valence scenarios. This study provides a critical first step towards identifying evidence-based indicators of short-term emotional states in dogs, while highlighting considerations that should be made when employing these parameters, including the influence of coder bias, food provision, exercise, and external temperature. Overall, it is recommended future dog emotion and welfare research use a combination of parameters including indicators of both emotional valence and arousal.

Daily dosing of cannabidiol (CBD) demonstrates a positive effect on measures of stress in dogs during repeated exposure to car travel.

Dogs are routinely exposed to events that may elicit stress and result in negative emotional states which can impact pet dog welfare. One event many dogs living with people are routinely exposed to is car travel, with many dogs displaying behaviors, along with corresponding physiological responses, that are indicative of stress and anxiety. There are a range of management and treatment options that exist from behavior modification, drug therapy, and supplements, often with varying results. The aim of this study was to evaluate whether multiple doses of a tetrahydrocannabinol-free cannabidiol (CBD) distillate over a period of 6 mo could positively influence measures of stress in dogs. In a blinded, parallel design study, dogs (n = 19) underwent a series of short car journeys (test) where a range of physiological and behavioral measures were collected pre, during, and post-test. The car journeys elicited stress in this population of dogs, as indicated by significant changes (P < 0.05) in several stress-related measures (serum cortisol, heart rate, heart rate variability, whining, lip licking, yawning, and qualitative behavioral ratings) observed from baseline to test, which persisted over repeated car travel events. The mitigating effect of CBD treatment varied by measure, with cortisol, whining, lip licking, and qualitative behavioral ratings indicating a significant (P < 0.05) reduction in canine stress compared to the placebo group for at least one time point. Additional research investigating a range of dog populations and stressors is required to fully understand the complex effect of CBD on canine emotional wellbeing.

Pet dogs often experience stress during routine car travel which can negatively affect both dogs and humans. A range of management and treatment options exist to address this, such as behavior modification, drug therapy, and supplements, yielding mixed results. Dog owners often seek strategies that are easy to administer and are effective in improving their pet's well-being. The objective of this study was to investigate the effect daily dosing of cannabidiol (CBD) over a 6-mo period, had on measures of stress in dogs. In a parallel design study, dogs experienced short car journeys (test) and a range of physiological and behavioral measures were collected pre, during, and post-test. Significant changes in several stress-related measures including serum cortisol, heart rate, heart rate variability, whining, lip licking, yawning, and qualitative behavioral ratings were observed from baseline to test, indicating that car travel was stressful in this population. The impact of CBD treatment varied across these measures, with certain indicators showing a marked reduction in canine stress when compared to a placebo group. Additional research is required to fully understand the complex effect CBD has on the emotional well-being of dogs.

A single dose of cannabidiol (CBD) positively influences measures of stress in dogs during separation and car travel.

Many dogs experience stress when separated from their caregivers, as well as when traveling in vehicles. Pet owners employ various approaches to managing these issues, from training, to giving medications and supplements, often with mixed results. Cannabidiol (CBD) can alleviate stress and anxiety in humans but the effect it has on canine stress is less well-documented. The present study aimed to understand the impact of being left alone and traveling in a car on measures of canine stress, and establish whether a single dose of a tetrahydrocannabinol (THC)-free CBD distillate could positively influence any measures of stress. In a blinded, parallel design study, a population of dogs were either left alone in a familiar room (n = 21) or underwent a short car journey (n = 19). A range of physiological and behavioral measures were collected pre, during and post-test. Significant changes in several stress-related measures (serum cortisol, mean ear temperature, heart rate, heart rate variability, whining and a stressed/anxious behavioral factor) were observed from baseline to test, with the car journey test paradigm eliciting a more pronounced stress response overall. The mitigating effect of CBD treatment varied by measure and test, with some indicating a significant reduction in canine stress compared to the placebo group. Additional research is required to fully understand the complex effect of CBD on canine wellbeing.

Long-Lasting Chews Elicit Positive Emotional States in Dogs during Short Periods of Social Isolation.

Dogs may experience negative emotional states when isolated from human caregivers and conspecifics. This study aimed to evaluate how dogs interact with different enrichments during a short period of social isolation, as a first step towards identifying methods for improving their emotional wellbeing. Using a cross-over design, dogs (n = 20) at the Waltham Petcare Science Institute were exposed to four different food-based enrichments while left alone in a familiar room for 20 min: long-lasting chew (Chew), kibble in a treat-dispensing toy (Toy), and kibble dispensed through a smart treat-dispensing device with (Device + Voice) and without (Device) a person talking to the dog. Time spent engaging with each enrichment item and emotional valence and arousal (7-point scale collected every 5-min) were scored from videos. The results of linear mixed models indicated Chew was the most successful enrichment, with dogs having lower arousal scores (p < 0.05 vs. Device and Toy) during the first five minutes of isolation, higher positive valence scores (p < 0.05 vs. all) during the second five minutes of isolation, and spending the most total time engaged (p < 0.01 vs. all). Based on these findings, long-lasting chews should be further explored to assess their impact on dog emotional wellbeing.

Veterinary background noise elicits fear responses in cats while freely moving in a confined space and during an examination.

Many cats show signs of fear and stress during veterinary examinations and procedures, with environmental stimuli such as noise contributing to these responses. The objective of this study was to investigate the effect of background noise commonly heard in veterinary clinics (people talking, kennel doors shutting, dogs barking) on behavioural and physiological responses in companion cats. In Experiment 1, owned cats underwent a mock physical examination in a veterinary clinic with (n = 16) or without (n = 16) a pre-recorded noise track. A second experiment was conducted to assess cat responses to noise outside of handling and a clinic environment. In Experiment 2, shelter cats were either exposed (n = 15) or not exposed (n = 15) to the same noise track while allowed free movement in a small enclosure. Physiological and behavioural responses previously validated as negative responses in cats (e.g., indicative of fear, stress and aversion) were recorded, and outcome variables were compared between treatments. For both experiments, cats exposed to the noise track showed higher heart and respiratory rates. Noise was not associated with behavioural changes during the physical examination; however, cats who were freely moving showed more behavioural signs of fear and/or stress during noise exposure compared to the no noise conditions. These results show that high levels of background noise elicit physiological stress responses in cats, while ceiling effects of the examination and exposure to the clinic environment likely prevented treatment-related behavioral differences from being detected during the physical examination. It is recommended that those working with cats in environments with high levels of background noise limit cat exposure to these noises to reduce cat fear and stress.

Effect of Music on Stress Parameters in Dogs during a Mock Veterinary Visit.

Veterinary visits can be stressful for dogs, but how their wellbeing changes during a visit is not well understood. Music therapy has been successfully used in clinical practice to alleviate stress and anxiety in people. The present study aimed to understand how canine stress changes during a veterinary visit, establish the effect of music, and highlight measures which may be of practical use. In a randomized crossover design, dogs were exposed to no music and a bespoke piece of classical music at a tempo designed to match their resting heart rate during a mock veterinary visit. Dogs were scored as more "afraid" during the physical examination compared to when they were in the hospital kennel (p < 0.001). Salivary cortisol, IgA, and infrared temperature all increased significantly (p < 0.05) from baseline to post-kennel and post-examination, with no effect of music treatment. Core body temperature (p = 0.010) and the odds of 'relaxed' lips (p = 0.020) were lower when dogs were exposed to music compared to control visits. Overall, dogs experienced changes in physiology and behavior, indicative of increased stress, over the course of the visit. Additional research is required to further understand the effect that bespoke music may have in alleviating canine stress during veterinary visits.

Characteristics of Loads of Cattle Stopping for Feed, Water and Rest during Long-Distance Transport in Canada.

This study is the first comprehensive examination of long-haul cattle being transported across Canada and off-loaded for feed, water and rest. A total of 129 truckloads were observed at one of two commercial rest stations near Thunder Bay, Ontario. Data collected included information regarding the truck driver, the trailer, the trip, the animals and animal handling. The majority of the loads stopping were feeder calves (60.94%) while 21.09% were weaned calves, and the remaining 14.84% were market weight cattle. The truck loads surveyed were in transit for, on average, 28.2 ± 5.0 hours before stopping and cattle were rested for an average of 11.2 ± 2.8 hours. These data suggest that loads stopping at the rest station were adhering to the regulations stated in the Health of Animals Act, which outline a maximum of 48 hours in transit before a mandatory stop of at least 5 hours for feed, water and rest. There was a large amount of variability around how well recommendations, such as stocking density were followed. Further research is required to assess how well cattle are coping with long-distance transport under current regulations and industry practices.