ABSTRACT
A comparative data of two generally accepted mass techniques for estimate the molecular weight distribution of polysaccharides are presented. The data were obtained from agave fructans samples of different molecular weight, which were analyzed authored independently. The two analytical techniques used were Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-ToF) and High Pressure Size Exclusion Chromatography (HP-SEC). The data set here are related to the research paper entitled "Size-exclusion chromatography (HPLC-SEC) technique optimization by simplex method to estimate molecular weight distribution of agave fructans" by Moreno-Vilet et al. [1]. This article present the comparative figures as histograms obtained from mathematically processed MALDI-ToF spectra and HPLC-SEC chromatograms. And also, the calculated polymer parameters as number and mass molecular weight, number and mass average degree of polymerization and dispersity index.
ABSTRACT
Agave fructans are increasingly important in food industry and nutrition sciences as a potential ingredient of functional food, thus practical analysis tools to characterize them are needed. In view of the importance of the molecular weight on the functional properties of agave fructans, this study has the purpose to optimize a method to determine their molecular weight distribution by HPLC-SEC for industrial application. The optimization was carried out using a simplex method. The optimum conditions obtained were at column temperature of 61.7°C using tri-distilled water without salt, adjusted pH of 5.4 and a flow rate of 0.36mL/min. The exclusion range is from 1 to 49 of polymerization degree (180-7966Da). This proposed method represents an accurate and fast alternative to standard methods involving multiple-detection or hydrolysis of fructans. The industrial applications of this technique might be for quality control, study of fractionation processes and determination of purity.
