Extracellular Acetylated Histone 3.3 Induces Inflammation and Lung Tissue Damage.

Extracellular histones, part of the protein group known as damage-associated molecular patterns (DAMPs), are released from damaged or dying cells and can instigate cellular toxicity. Within the context of chronic obstructive pulmonary disease (COPD), there is an observed abundance of extracellular histone H3.3, indicating potential pathogenic implications. Notably, histone H3.3 is often found hyperacetylated (AcH3.3) in the lungs of COPD patients. Despite these observations, the specific role of these acetylated histones in inducing pulmonary tissue damage in COPD remains unclear. To investigate AcH3.3's impact on lung tissue, we administered recombinant histones (rH2A, rH3.3, and rAcH3.3) or vehicle solution to mice via intratracheal instillation. After 48 h, we evaluated the lung toxicity damage and found that the rAcH3.3 treated animals exhibited more severe lung tissue damage compared to those treated with non-acetylated H3.3 and controls. The rAcH3.3 instillation resulted in significant histological changes, including alveolar wall rupture, epithelial cell damage, and immune cell infiltration. Micro-CT analysis confirmed macroscopic structural changes. The rAcH3.3 instillation also increased apoptotic activity (cleavage of caspase 3 and 9) and triggered acute systemic inflammatory marker activation (TNF-α, IL-6, MCP-3, or CXCL-1) in plasma, accompanied by leukocytosis and lymphocytosis. Confocal imaging analysis confirmed lymphocytic and monocytic/macrophage lung infiltration in response to H3.3 and AcH3.3 administration. Taken together, our findings implicate extracellular AcH3.3 in inducing cytotoxicity and acute inflammatory responses, suggesting its potential role in promoting COPD-related lung damage progression.

Strategic self-limiting production of infectious HIV particles by CRISPR in permissive cells.

Post-translational glycosylation of the HIV-1 envelope protein involving precursor glycan trimming by mannosyl oligosaccharide glucosidase (MOGS) is critically important for morphogenesis of virions and viral entry. Strategic editing of the MOGS gene in T lymphocytes and myeloid origin cells harboring latent proviral DNA results in the production of non-infectious particles upon treatment of cells with latency reversal agents. Controlled activation of CRISPR-MOGS by rebound HIV-1 mitigates production of infectious particles that exhibit poor ability of the virus to penetrate uninfected cells. Moreover, exclusive activation of CRISPR in cells infected with HIV-1 alleviates concern for broad off-target impact of MOGS gene ablation in uninfected cells. Combination CRISPR treatment of peripheral blood lymphocytes prepared from blood of people with HIV-1 (PWH) tailored for editing the MOGS gene (CRISPR-MOGS) and proviral HIV-1 DNA (CRISPR-HIV) revealed a cooperative impact of CRISPR treatment in inhibiting the production of infectious HIV-1 particles. Our design for genetic inactivation of MOGS by CRISPR exhibits no detectable off-target effects on host cells or any deleterious impact on cell survival and proliferation. Our findings offer the development of a new combined gene editing-based cure strategy for the diminution of HIV-1 spread after cessation of antiretroviral therapy (ART) and its elimination.

Leishmania spp Epitopes in Humans Naturally Resistant to the Disease: Working Toward a Synthetic Vaccine.

Vaccines are one of the most effective strategies to fight infectious diseases. Reverse vaccinology strategies provide tools to perform in silico screening and a rational selection of potential candidates on a large scale before reaching in vitro and in vivo evaluations. Leishmania infection in humans produces clinical symptoms in some individuals, while another part of the population is naturally resistant (asymptomatic course) to the disease, and therefore their immune response controls parasite replication. By the identification of epitopes directly in humans, especially in those resistant to the disease, the probabilities of designing an effective vaccine are higher. The aim of this work was the identification of Leishmania epitopes in resistant humans. To achieve that, 11 peptide sequences (from Leishmania antigenic proteins) were selected using epitope prediction tools, and then, peripheral blood mononuclear cells (PBMCs) were isolated from human volunteers who were previously divided into four clinical groups: susceptible, resistant, exposed and not exposed to the parasite. The induction of inflammatory cytokines and lymphoproliferation was assessed using monocyte-derived dendritic cells (moDCs) as antigen-presenting cells (APCs). The response was evaluated after exposing volunteers' cells to each peptide. As a result, we learned that STI41 and STI46 peptides induced IL-8 and IL-12 in moDCs and lymphoproliferation and low levels of IL-10 in lymphocytes differentially in resistant volunteers, similar behavior to that observed in those individuals to L. panamensis lysate antigens. We conclude that, in silico analysis allowed for the identification of natural Leishmania epitopes in humans, and also STI41 and STI46 peptides could be epitopes that lead to a cellular immune response directed at parasite control.

Molecular Characterization of Tc964, A Novel Antigenic Protein from Trypanosoma cruzi.

The Tc964 protein was initially identified by its presence in the interactome associated with the LYT1 mRNAs, which code for a virulence factor of Trypanosoma cruzi. Tc964 is annotated in the T. cruzi genome as a hypothetical protein. According to phylogenetic analysis, the protein is conserved in the different genera of the Trypanosomatidae family; however, recognizable orthologues were not identified in other groups of organisms. Therefore, as a first step, an in-depth molecular characterization of the Tc946 protein was carried out. Based on structural predictions and molecular dynamics studies, the Tc964 protein would belong to a particular class of GTPases. Subcellular fractionation analysis indicated that Tc964 is a nucleocytoplasmic protein. Additionally, the protein was expressed as a recombinant protein in order to analyze its antigenicity with sera from Chagas disease (CD) patients. Tc964 was found to be antigenic, and B-cell epitopes were mapped by the use of synthetic peptides. In parallel, the Leishmania major homologue (Lm964) was also expressed as recombinant protein and used for a preliminary evaluation of antigen cross-reactivity in CD patients. Interestingly, Tc964 was recognized by sera from Chronic CD (CCD) patients at different stages of disease severity, but no reactivity against this protein was observed when sera from Colombian patients with cutaneous leishmaniasis were analyzed. Therefore, Tc964 would be adequate for CD diagnosis in areas where both infections (CD and leishmaniasis) coexist, even though additional assays using larger collections of sera are needed in order to confirm its usefulness for differential serodiagnosis.

Confiabilidad y validez de la escala para medir la contribución del cuidador al autocuidado del paciente con insuficiencia cardiaca / Reliability and validity of the scale to measure the caregiver's contribution to self-care of the patient with heart failure / Confiabilidade e validade da escala para mensurar a contribuição do cuidador para o autocuidado do paciente com insuficiência cardíaca

OBJETIVO: Determinar la confiabilidad y validez de la versión en español de la escala para medir la contribución del cuidador al autocuidado del paciente con falla cardiaca (CC-SCHFI). METODOLOGÍA: Estudio metodológico que incluyó pruebas psicométricas, aplicadas a cuidadores familiares de personas con insuficiencia cardiaca. Se siguieron las recomendaciones metodológicas de la International Test Commission para la adaptación cultural de la prueba traducción - retrotraducción, adecuación semántica, validez facial y de contenido, confiabilidad mediante la consistencia interna y validez de constructo). RESULTADOS: Se obtuvo un acuerdo entre expertos según el Kappa de Fleiss de 0,44 en la dimensión de relevancia, 0,41 en la dimensión de pertinente y una validez de contenido por método normalizado de CVI de 0,68. La consistencia interna del instrumento resultó óptima con un coeficiente de Alpha de Cronbach = 0,950. La exploración del constructo arrojó una estructura de 6 componentes. CONCLUSIONES: Se obtuvo una escala en español disponible para abordar el fenómeno de la contribución del cuidador al autocuidado del paciente con falla cardiaca, con un análisis factorial que mostró que los ítems del instrumento tienden a agruparse en dos dominios (de mantenimiento y gestión del autocuidado) y no tres, como lo propone el instrumento original

OBJETIVO: Determinar a confiabilidade e a validade da versão em espanhol da escala para medir a contribuição do cuidador ao autocuidado de pacientes com insuficiência cardíaca (CC-SCHFI). METODOLOGIA: Estudo metodológico, com abordagem quantitativa; que incluiu testes psicométricos, aplicados a familiares cuidadores de pessoas com insuficiencia cardíaca. As recomendações metodológicas da International Test Commission foram seguidas para a adaptação cultural do teste (tradução-retroversão, adequação semântica, validade facial e de conteúdo, confiabilidade por consistência interna e validade de constructo). RESULTADOS: Houve concordância entre os especialistas segundo o Fleiss Kappa de 0,44 na dimensão de relevância, 0,41 na dimensão relevante e validade de conteúdo pelo método CVI padronizado de 0,68. A consistência interna do instrumento foi ótima com um coeficiente alfa de Cronbach = 0,950. A exploração do construto produziu uma estrutura de 6 componentes. CONCLUSÕES: Foi disponibilizada uma escala de espanhol para abordar o fenômeno da contribuição do cuidador para o autocuidado de pacientes com insuficiência cardíaca, com uma análise fatorial que mostrou que os itens do instrumento tendem a ser agrupados em dois domínios (manutenção e manejo dos pacientes). autocuidado) e não três, como proposto pelo instrumento original

OBJECTIVE: To determine the reliability and validity of the Spanish version of the scale to measure the caregiver's contribution to self-care of patients with heart failure (CC-SCHFI). METHODOLOGY: Methodological study, with quantitative approach; which included psychometric tests, applied to family caregivers of people with heart failure. The methodological recommendations of the International Test Commission were followed for the cultural adaptation of the translation-back-translation test, semantic adequacy, facial and content validity, reliability through internal consistency and construct validity). RESULTS: An agreement was reached between experts according to the Fleiss Kappa of 0.44 in the relevance dimension, 0.41 in the relevant dimension and a content validity by standardized CVI method of 0.68. The internal consistency of the instrument was optimal with a Cronbach's Alpha coefficient = 0.950. The exploration of the construct yielded a structure of 6 components. CONCLUSIONS: A Spanish scale was available to address the phenomenon of the caregiver's contribution to the self-care of patients with heart failure, with a factorial analysis that showed that the items of the instrument tend to be grouped into two domains (maintenance and management of self-care) and not three, as proposed by the original instrument

Self-efficacy in physical activity in children, body mass index and physical activity level of their parents / Autoeficacia en la actividad física en niños, índice de masa corporal y nivel de actividad física de sus padres

Abstract Objective: To determine the relationship between self-efficacy in physical activity in children from 7 to 10 of a school in Bogotá, their body mass index and physical activity levels of parents. Method: Correlative descriptive quantitative study, with a sample of 60 boys and girls between 7 to 10 and 11 months and their biological parents. For the collection of the information, a questionnaire was used to evaluate the self-efficacy towards physical activity in children and the International Physical Activity Questionnaire, long version. The analysis revolved around the correlation between the main variables supported by the Spearman and Gamma correlation coefficient. The explanatory models were based on the multiple regression analysis. Results: The relationship between self-efficacy and children's BMI was negative (Spearman's coefficient of relation - 0.107 and Gamma correlation coefficient of -, 083). The relationship between the body mass index of the children and the physical activity level of their mothers was negative, (Spearman coefficient of relationship of 0.141, and a Gamma correlation coefficient of -, 113). The relationship between the self-efficacy towards the physical activity of the children and the physical activity level of their mothers was negative, (Spearman coefficient of 0.063, and Gamma of -, 160). Conclusion: There is little statistical significance in the relationship between self-efficacy in physical activity in children, their body mass index and physical activity levels of parents.

Resumen Objective: Determinar la relación entre la autoeficacia en actividad física en niños de 7 a 10 años de un colegio de Bogotá, su índice de masa corporal y los niveles de actividad física de los padres. Método: Estudio cuantitativo descriptivo correlational, con una muestra de 60 niños y niñas entre 7 a 10 años 11 meses y sus padres biológicos. Para la recolección de la información se utilizaron el Cuestionario para evaluar la autoeficacia hacia la actividad física en niños y el Cuestionario Internacional de Actividad Física versión larga. El análisis giró alrededor de la correlación entre las variables principales apoyadas en el coeficiente de correlación Spearman y Gamma. Los modelos explicativos se fundamentaron en el análisis de regresión múltiple. Resultados: La relación entre la autoeficacia y el IMC de los niños fue negativa (coeficiente de relación de Spearman - 0,107 y coeficiente de correlación Gamma de -,083). La relación entre el índice de masa corporal de los niños y el nivel de actividad física de sus madres fue negativa, (coeficiente de relación de Spearman de 0,141, y un coeficiente de correlación Gamma de -,113). La relación entre la autoeficacia hacia la actividad física de los niños y el nivel de actividad física de sus madres fue negativa, (coeficiente de relación de Spearman 0,063, y Gamma de -,160). Conclusión: Existe poca significancia estadística en la relación entre la autoeficacia en actividad física en los niños, su índice de masa corporal y los niveles de actividad física de los padres.

Synthetic Peptides Derived from Ribosomal Proteins of Leishmania spp. in Mucocutaneous Leishmaniasis: Diagnostic Usefulness.

BACKGROUND: The serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. OBJECTIVE: This study was thus aimed at evaluating synthetic peptides derived from Leishmania braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA Methods: It was used 8 and 13 peptides derived from ribosomal proteins 25 and S5 respectively as antigens in order to detect IgG antibodies by ELISA in people with active MCL, Chagas disease (CH) and autoimmune disease (AID). RESULTS: 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21.7%, 13.04%, 20% and 20%, respectively) as well as having 95%, 100%, 100% and 82.5% specificity, respectively. CONCLUSION: The study revealed the limited usefulness of the peptides being studied as a diagnostic tool in the conditions used here, because its low sensitivity, but it is worth highlighting that the use of peptides as antigen in the serodiagnosis of MCL may overcome the cross reaction presented with other antigens, thus avoiding false positives.

Adherencia al tratamiento en la insuficiencia cardiaca y las tecnologías de la información y la comunicación / Treatment adherence in heart failure and information and communication Technologies

Resumen Objetivo: Determinar la efectividad del uso de las tecnologías de la información y la comunicación, en la adherencia al tratamiento de los pacientes con insuficiencia cardiaca, que asistieron a una Unidad de Insuficiencia Cardiaca de Bogotá. Métodos: Se realizó un ensayo clínico con preprueba, posprueba y grupo de control. Se conformaron aleatoriamente tres grupos de 37 participantes cada uno, a dos de los cuales se les aplicó una intervención durante dos meses basada en el uso de las tecnologías de la información y la comunicación; el grupo 1 recibió seguimiento telefónico más envío de mensajes de texto, el grupo 2 seguimiento telefónico más envío de correos electrónicos y el grupo 3 correspondiente al grupo de control recibió la intervención de enfermería usual en una Unidad de Insuficiencia Cardiaca. Resultados: Los tres grupos definidos para el estudio modificaron significativamente sus niveles medios de adherencia; en los grupos 1 y 2 dicho incremento es atribuible a la intervención con el uso de las tecnologías de la información y la comunicación, de igual manera la adherencia en los participantes del grupo control que recibieron la atención usual en una Unidad de Insuficiencia Cardiaca, también muestra un incremento significativo. Conclusiones: El uso de las tecnologías de la información y la comunicación: el seguimiento telefónico asociado a los mensajes de texto y los correos electrónicos incrementó la adherencia al tratamiento farmacológico y no farmacológico en pacientes con insuficiencia cardiaca a través de la motivación y el seguimiento de los pacientes.

Abstract Motivation: To determine the efficacy of the use of information and communication technologies in the treatment adherence of patients with heart failure that were assisted at the Heart Failure Unit of Bogotá. Methods: A clinical trial was conducted with pre-, post-test and control group. Three randomised groups of 37 participants each were conformed, two of which received an intervention during two months based in the use of information and communication technology; group 1 received telephone follow-up plus text messages, group 2 telephone follow-up plus emails and group 3, which was the control group, received the usual nurse follow-up at a Heart Failure Unit. Results: The three defined study groups significantly modified their average adherence levels; groups 1 and 2 the increase is attributable to the intervention with the use of information and communication technologies, likewise, adherence of the control group participants who received the usual care at a Heart Failure Unit also showed a significant increase. Conclusion: The use of information and communication technologies, such as the telephone follow-up associated to text messages and emails, rose the adherence to the pharmacological and non-pharmacological treatment in patients with heart failure by motivating and monitoring patients.

Agencia de autocuidado y factores de riesgo cardiovascular en adolescentes / Agência de autocuidado e fatores de risco cardiovascular em adolescentes

Objetivo: Este estudio tuvo como objetivo determinar la relación entre la capacidad de agencia de autocuidado y los factores de riesgo cardiovascular en adolescentes de una Institución de Educación Superior en el Distrito de Barranquilla, Colombia. Metodología: Estudio descriptivo, exploratorio, de corte transversal, con abordaje cuantitativo, basado en la Teoría del Déficit de Autocuidado de Dorothea Orem. La muestra fue compuesta por 133 adolescentes, seleccionados mediante muestreo aleatorio simple. Se aplicaron la Escala Apreciación de la Agencia de Autocuidado (segunda versión en español), el Cuestionario Internacional de Actividad Física (versión corta) y la Encuesta Mundial de Tabaquismo en Jóvenes. Para el análisis de los niveles de significancia se utilizó la prueba de Kruskal-Wallis para muestras independientes. Resultados: La mayor parte de los participantes presentó niveles de capacidad de agencia de auto-cuidado media y alta respectivamente. De acuerdo con la identificación de los factores de riesgo para enfermedad cardiovascular, se evidenció que los adolescentes presentaron probabilidades de desarrollar alguna enfermedad cardiovascular hacia el futuro, dado el comportamiento de los factores de riesgo, como son la obesidad y el sedentarismo. En cuanto a la relación entre la capacidad de agencia de autocuidado y los factores de riesgo, se encontró asociación significativa al relacionar la capacidad de agencia de autocuidado con las categorías de clasificación de la presión arterial. Conclusiones: Los resultados encontrados en el presente estudio coinciden con lo hallado por otros investigadores que afirman que el sedentarismo y la obesidad son los factores de riesgo con mayor presencia en los adolescentes. De igual manera, los hallazgos indican que los adolescentes mostraron capacidad de agencia de autocuidado de nivel medio, pero sólo se encontró asociación significativa entre la capacidad de agencia de autocuidado con las categorías de clasificación de la presión arterial.

Resumo Objetivo: Este estudo teve como objetivo determinar a relação entre a capacidade de agência de autocuidado e fatores de risco cardiovascular em adolescentes em uma instituição de ensino superior no Distrito de Barranquilla, Colômbia. Metodologia: Estudo descritivo e exploratório, de corte transversal, com abordagem quantitativo, baseado na Teoria do Déficit de Autocuidado de Dorothea Orem. A amostra foi composta por um grupo de 133 adolescentes, selecionados por amostragem aleatória simples. Aplicaram-se a Escala de Apreciação da Agência de Autocuidado (segunda versão em espanhol), o Questionário Internacional de atividade física, (versão curta) e Global Youth Tobacco Survey. Para a análise dos níveis de significância foi utilizado o teste de Kruskal-Wallis para amostras independentes. Resultados: A maior proporção de participantes apresentou, respectivamente, níveis médios e altos de capacidade de agência de autocuidado. De acordo com a identificação de fatores de risco para doença cardiovascular, evidenciou-se que os adolescentes têm probabilidades de desenvolver alguma doença cardiovascular no futuro, devido ao comportamento dos fatores de risco como a obesidade e o sedentarismo. No que respeita à relação entre a capacidade de agência de autocuidado e fatores de risco, evidenciou-se associação significativa ao relacionar a capacidade da agência de autocuidado com as categorias de classificação da pressão arterial. Conclusões: Os resultados deste estudo são consistentes com o que foi encontrado por outros pesquisadores, que afirmam que o sedentarismo e a obesidade são fatores de risco com maior presença em adolescentes. Do mesmo modo, os resultados indicam que os adolescentes mostraram capacidade da agência de autocuidado de nível médio, porém só foi encontrada relação significativa entre a capacidade da agência de autocuidado com as categorias de classificação da pressão arterial.

Objective: This study aimed to determine relationship between the ability of self-care agency and cardiovascular risk factors in adolescents from a higher education institution in Distrito de Barranquilla, Colombia. Methodology: Descriptive, exploratory, cross-sectional study with a quantitative approach, based on the Self-care deficit theory of Dorothea Orem. 133 adolescents sample size was selected by simple random sampling. The Appraisal of the Self-care Agency (second version in spanish), the Global Youth Tobacco Survey and International Physical Activity Questionnaire Short Form. For the analysis of significance levels, the Kruskal-Wallis test was used for independent samples. Results: The majority of participants presented levels respectively medium and high of ability of self-care agency. According to identification of cardiovascular risk factors disease, we found that adolescents presented chances of developing cardiovascular disease to the future, given the behavior of risk factors such as obesity and sedentary lifestyle. With regard to the relationship between ability of self-care agency and risk factors, we found significant association relating the ability of self-care agency with the classification categories of blood pressure. Conclusions: The results from our study are consistent with findings of researchers who assert that sedentary lifestyle and obesity are risk factors with greater presence in adolescents. Similarly, our findings indicate that adolescents presented medium-level ability self-care agency, but we only found significant association between ability self-care agency with classification categories of blood pressure.

Adherencia al tratamiento y calidad de vida en personas con infarto agudo de miocardio / Adherence to Treatment and Quality of Life in People with Acute Myocardial Infarction / Adesão ao tratamento e qualidade de vida em pessoas com infarto agudo de miocárdio

Objetivo: identificar la asociación entre adherencia al tratamiento y calidad de vida relacionada con la salud (CVRS) en población con infarto agudo de miocardio (IAM). Materiales y método: estudio cuantitativo de diseño descriptivo correlacional de corte transversal. Se eligieron 180 participantes por muestreo de tipo probabilístico con selección aleatoria simple. Para el análisis estadístico, se utilizó estadística no paramétrica con correlación de Spearman. Resultados: la edad media fue de 63 años, el promedio de tiempo transcurrido después del infarto fue de 35 meses. Frente al nivel de adherencia al tratamiento, predominó la adherencia total (54 %), adherencia parcial (45%); en cuanto a la calidad de vida relacionada con la salud, el 27 % tuvo un bajo nivel; respecto a la correlación de Spearman, se estableció un coeficiente de -0,315 con una significancia de p=0,00, donde el número negativo es reflejo de la naturaleza de las escalas con las que fueron medidas las variables, por lo que la correlación es positiva, significativa, pero numéricamente modesta. Conclusión: la asociación entre adherencia al tratamiento y calidad de vida relacionada con la salud evidencia en una correlación estadísticamente significativa, pero catalogada como modesta, siendo estos resultados coherentes con la multidimensionalidad de los fenómenos.

Objective: To identify the relationship between adherence to treatment and health-related quality of life (HRQoL) in people with acute myocardial infarction (AMI). Materials and method: Quantitative study of cross-sectional, descriptive and correlational design. The researchers used non-probability simple random sampling to select 180 participants and Spearman's nonparametric correlation for statistical analysis. Results: The average age was 63 years, and the average time elapsed after infarction was 35 months. Regarding level of adherence to treatment, total adherence (54%) predominated over partial adherence (45%). As to health-related quality of life, 27% showed a low level. Regarding Spearman's correlation, a coefficient of -0.315 with a significance of p = 0.00 was established, where the negative number reflects the nature of the scales with which variables were measured, and therefore the correlation was positive, numerically modest but significant. Conclusion: The relationship between adherence to treatment and health-related quality of life is evident in a correlation that is statistically significant but catalogues as modest, and these results are consistent with the multidimensionality of the phenomena.

Objetivo: identificar a associação entre adesão ao tratamento e qualidade de vida relacionada com a saúde (QVRS) em população com infarto agudo de miocárdio (IAM). Materiais e método: estudo quantitativo de desenho descritivo correlacional de corte transversal. Escolheram-se 180 participantes por amostra de tipo probabilística com seleção aleatória simples. Para a análise estatística, utilizou-se estatística não paramétrica com correlação de Spearman. Resultados: a idade média foi de 63 anos, a média de tempo transcorrido depois do infarto foi de 35 meses. Diante do nível de adesão ao tratamento, predominou a adesão total (54%), adesão parcial (45%); quanto à qualidade de vida relacionada com a saúde, 27% tiveram um baixo nível; a respeito da correlação de Spearman, estabeleceu-se um coeficiente de -0,315 com uma significancia de p = 0,00, em que o número negativo é reflexo da natureza das escalas com as quais as variáveis foram medidas, por isso a correlação é positiva, significativa, mas numericamente modesta. Conclusão: a associação entre adesão ao tratamento e qualidade de vida relacionada com a saúde evidencia uma correlação estatisticamente significativa, mas catalogada como modesta, sendo esses resultados coerentes com a multidimensionalidade dos fenômenos.

La enfermería y el cuidado para la salud cardiovascular: análisis de concepto / The nursing and the care for the cardiovascular health: concept analysis

La enfermería se ha preocupado por ampliar su marco conceptual basándose en el análisis de conceptos y la investigación de fenómenos propios. Según Morse, "Las técnicas de análisis de concepto se han convertido en una parte estándar de los programas de doctorado en enfermería. A pesar de estas ventajas, hay una gran cantidad de exploración conceptual que todavía no se ha logrado" (1). Este artículo argumenta el análisis del concepto de salud cardiovascular, utilizando la revisión de literatura y entrevistas a profesionales de la salud y a personas cercanas al fenómeno de la enfermedad cardiovascular en una ciudad del departamento de Córdoba, Colombia. El propósito es ilustrar un análisis que oriente al/a enfermero/a en el abordaje del cuidado de la salud cardiovascular de forma efectiva y acorde con las necesidades de las personas. Para la exploración conceptual, se utilizó el método de Rodgers (2, 3), quien propuso el punto de vista evolutivo para el desarrollo de conceptos a partir de datos brutos (literatura publicada o entrevistas) considerando el contexto (antecedentes, consecuencias y relaciones con otros conceptos). Al explorar los conceptos, es evidente que no hay claridad en el significado de la salud cardiovascular, tanto para las personas que viven en situación de enfermedad cardiovascular como para los profesionales de la salud, lo que corrobora la importancia del análisis para futuros procesos de clarificación e identificación conceptual. En este sentido, se esboza una propuesta de definición del concepto explorado, con los principales atributos para su comprensión desde una visión disciplinar bajo los supuestos de la Teoría de Dorothea Orem.

Abstract The nursing has worried to extend its conceptual frame, being based on the analysis of concepts and the investigation of the own phenomena. According to Morse, "the techniques of concept analysis have become a standard part of the programs of doctorate in infirmary; in spite of these advantages, there is a great amount of conceptual exploration that has still not been obtained". This article argues the analysis of the concepts of cardiovascular health, using like the revision of literature and interviews to professionals of health and people who live in situation of cardiovascular disease in a city of the Cordoba department, Colombia, in order to identify what the people think about this concept, sufficiently to illustrate an analysis that orients to the nurse in the boarding of the care of the cardiovascular health of an effective and in agreement with the needs of the people. For the conceptual exploration, we used the method of Rodgers (2, 3), who proposed the evolutionary point of view for the development of concepts from gross data (published literature or interview), considering context (antecedents, consequences and relations with other concepts). When exploring the analysis of the concepts, it is evident that there is no clarity in the concept cardiovascular health, as much in the group of people who live in situation of cardiovascular disease like in the professionals of the health, which corroborates the importance of the analysis for the unification of concepts. This is a reason why a new definition of the concept is explored, with its main attributes for the understanding from a vision sets out to discipline under the assumptions of the theory of Dorothea Orem.

A enfermagem tem se preocupado por ampliar seu marco conceitual, baseando-se na análise de conceitos e a pesquisa de fenômenospróprios. Segundo Morse, "As técnicas de análise de conceito converteram-se em um padrão dos programas de doutorado em enfermagem. Apesar destas vantagens, háuma grande quantidade de exploraçãoconceitual que ainda não se conseguiu" (1). Este artigo argumenta a análise do conceito de saúde cardiovascular, utilizando a revisão de literatura e entrevistas a profissionais da saúde e a pessoas próximas ao fenômeno da doença cardiovascular em uma cidade do departamento de Córdoba, na Colômbia. O propósito é ilustrar uma análise que oriente ao/a enfermeiro/a na abordagem do cuidado da saúde cardiovascular, de forma efetiva e acorde àsnecessidades das pessoas. Para a exploraçãoconceitual, utilizou-se o método de Rodgers (2, 3), quem propôs o ponto de vista evolutivo para o desenvolvimento de conceitos a partir de dados brutos (literatura publicada ou entrevistas), considerando o contexto (antecedentes, consequências e relaciones com outros conceitos). Ao explorar os conceitos, evidenciou-se que não existe clareza no significado da saúde cardiovascular, tanto para as pessoas que vivem em situação de doença cardiovascular, como para os profissionais da saúde, o que corrobora a importância da análise para futuros processos de clarificação e identificaçãoconceitual. Neste sentido, esboça-se uma proposta de definição do conceito explorado, com os principais atributos para sua compreensão, desde uma visão disciplinar, sob os supostos da Teoria de Dorothea Orem.

Comodidad del paciente hospitalizado en un servicio de cirugía cuando se proveen medidas de comodidad que inolucran a la familia / Hospitalized patient comfort in a surgical service when the family is involved in the comfort measures

El presente estudio propuso determinar el grado de comodidad del paciente hospitalizado cuando las medidas de comodidad son aplicadas por parte de la familia. en el servicio de cirugía de la fundación Santafé de Bogotá, se realizó un estudio de tipo cuasiexperimental, durante los meses de julio a noviembre de 2000, con una muestra constituida por 30 paciente. El Cuestionario General de Comodidad (GCQ sigla en ingles), desarrollado por la doctora Katharien Kolcaba, permitió medir el grado de comodidad del paciente y determinar las necesidades antes y despues de la intervención de la familia. La familia fue orientada en cuales medidas de comodidad podia realizar sin poner en peligro la vida del paciente. Altos grados de comodidad fueron encontrados en los pacientes después de la intervención de la familia en el cuidado con determinadas medidas de comodidad. Estos hallazgos sugieren que la familia esta en capacidad de asumir determinadas responsabilidades en el cuidado del paciente, ayudándolo a afrontar su crisis de enfermedad y favoreciendo de esta manera un cuidado mas humano y mas hogareño en los hospitales.