Can quarantine plant-parasitic nematodes within wastes be managed by useful tools in a circular economy approach?

Seen as an integral part of sustainable development, circular economy represents a model of production and consumption notably based on the limitation of both resource wastage and environmental impact. Laboratories and commercial companies working on plant pathogens, in particular quarantine species, must effectively disinfect their waste to avoid disseminating these organisms. The methods used for waste disinfection can however incur high energy costs or pose environmental and human health hazards. Here, we tested the effectiveness of five disinfection methods - chlorination, heat treatment, composting, mesophilic methanation and waste stabilization ponds - on plant-parasitic nematodes belonging to the genera Globodera and Meloidogyne. For the widely used chlorination and heat treatment methods, we showed that they can be very effective in inactivating nematodes at relatively low chlorine doses and temperatures (60 °C-3 min and 50 °C-30 min), respectively. For the three other disinfection methods tested, initially designed for waste recycling, we obtained different levels of efficiency. Composting and mesophilic methanation (based on cattle or pig slurry) both led to the complete elimination of nematodes, even for short treatment durations. However, waste stabilization ponds showed contrasting results, ranging from virtually no effect to high levels of inactivation of nematodes. Our study demonstrates that it is possible to use more environmentally friendly disinfection methods to control plant-parasitic nematodes. In particular, this finding paves the way towards the treatment of infected plant materials using composting or methanation, providing that disinfection is still reached under other (real-life) treatment conditions, especially with other kinds of waste. Both composting and methanation recycle and thus valorize infected waste; they are viable alternatives to landfilling or incineration, thereby demonstrating the usefulness of a circular economy approach.

Diversity of plant parasitic nematodes characterized from fields of the French national monitoring programme for the Columbia root-knot nematode.

Plant parasitic nematodes are highly abundant in all agrosystems and some species can have a major impact on crop yields. To avoid the use of chemical agents and to find alternative methods to manage these pests, research studies have mainly focused on plant resistance genes and biocontrol methods involving host plants or natural enemies. A specific alternative method may consist in supporting non-damaging indigenous species that could compete with damaging introduced species to decrease and keep their abundance at low level. For this purpose, knowledge about the biodiversity, structure and functioning of these indigenous communities is needed in order to carry out better risk assessments and to develop possible future management strategies. Here, we investigated 35 root crop fields in eight regions over two consecutive years. The aims were to describe plant parasitic nematode diversity and to assess the potential effects of cultivation practices and environmental variables on communities. Community biodiversity included 10 taxa of plant parasitic nematodes. Despite no significant abundance variations between the two sampling years, structures of communities varied among the different regions. Metadata collected for the past six years, characterizing the cultural practices and soils properties, made it possible to evaluate the impact of these variables both on the whole community and on each taxon separately. Our results suggest that, at a large scale, many variables drive the structuration of the communities. Soil variables, but also rainfall, explain the population density variations among the geographical areas. The effect of the variables differed among the taxa, but fields with few herbicide applications and being pH neutral with low heavy metal and nitrogen concentrations had the highest plant parasitic nematode densities. We discuss how these variables can affect nematode communities either directly or indirectly. These types of studies can help to better understand the variables driving the nematode communities structuration in order to support the abundance of indigenous non-damaging communities that could compete with the invasive species.

The hidden diversity of the potato cyst nematode Globodera pallida in the south of Peru.

Our knowledge of the diversity of potato cyst nematodes in their native areas still remains patchy and should be improved. A previous study based on 42 Peruvian Globodera pallida populations revealed a clear south to north phylogeographic pattern, with five well-supported clades and maximum diversity observed in the south of Peru. In order to investigate this phylogeographic pattern more closely, we genotyped a larger collection of Peruvian populations using both cathepsin L gene sequence data and a new set of 13 microsatellite loci. Using different genetic analyses (STRUCTURE, DAPC), we consistently obtained the same results that led to similar conclusions: the presence of a larger genetic diversity than previously known suggesting the presence of cryptic species in the south of Peru. These investigations also allowed us to clarify the geographic borders of the previously described G. pallida genetic clades and to update our knowledge of the genetic structure of this species in its native area, with the presence of additional clades. A distance-based redundancy analysis (dbRDA) was also carried to understand whether there was a correlation between the population genetic differentiation and environmental conditions. This analysis showed that genetic distances observed between G. pallida populations are explained firstly by geographic distances, but also by climatic and soil conditions. This work could lead to a revision of the taxonomy that may have strong implications for risk assessment and management, especially on a quarantine species.

Changes in parasitoid communities over time and space: a historical case study of the maize pest Ostrinia nubilalis.

Understanding the ways in which human environmental modifications affect biodiversity is a key challenge in conservation planning, pest control and evolutionary ecology. Parasitoid communities, particularly those associated with agricultural pests, may be susceptible to such modifications. We document here changes in the larval parasitoid communities of Ostrinia nubilalis--the main pest of maize--and its sibling species O. scapulalis, based on two historical datasets, one collected from 1921-1928 and the other from 2001-2005. Each of these datasets encompasses several years and large geographical areas and was based on several thousands/millions of host larvae. The 80-year interval between the two datasets was marked by a decrease in O. nubilalis parasitism to about two thirds its initial level, mostly due to a decrease in the rate of parasitism by hymenopterans. However, a well balanced loss and gain of species ensured that species richness remained stable. Conversely, O. scapulalis displayed stable rates of parasitism over this period, with a decline in the species richness of its parasitoid community. Rates of parasitism and species richness in regions colonized by O. nubilalis during the 1950s were one half to one third those in regions displaying long-term colonisation by this pest. During the recent human activity-driven expansion of its range, O. nubilalis has neither captured native parasitoids nor triggered parasite spill back or spill over.