[Oxidative carbonylation of vascular wall proteins in dynamics of experimental venous thrombosis].

The objects of the study were a total of 24 conventional sexually mature Wistar rats weighing 200-400 g. Thrombosis was modelled by means of ligation of the common iliac vein. Animals were withdrawn from the study on days 1, 3 and 5 after intervention. The materials for the study in each animal were homogenates of the vein portion below the site of ligation (thrombosed vein) and the portion of the symmetrical vessel (intact vein). Taken as controls were portions of the common iliac vein of intact animals matched by age, body weight, and keeping conditions. The level of spontaneous and induced in the Fenton reaction oxidative carbonylation of proteins was determined by means of carbonyl derivatives according to the R.L. Levine technique modified by E.E. Dubinina with optical registration of the formed dinitrophenylhydrazines at 356, 370, 430 and 530 nm. The reserve-and-adaptation potential was assessed by means of counting the ratio of the amount of carbonyl derivatives of proteins in spontaneous and induced oxidation. The obtained findings showed that experimental thrombosis is accompanied and followed by an increase in the content of carbonyl derivatives of proteins in the wall of thrombosed veins and, to a lesser degree, in that of intact veins. The maximal elevation of the parameters was registered during the first 24 hours of the development of pathology, demonstrating not only early but late markers of oxidative modification of proteins. Thrombosed veins on day 3 were found to have a decrease in the content of carbonylated proteins to the level of the control values, which was associated with a maximal value of the reserve-adaptation potential. However, day five was marked by a secondary increase in carbonylation accompanied by certain exhaustion of the reserve-adaptive potential. In intact veins, a decrease of the spontaneous oxidative modification level on day 3 was accompanied by a splash of induced carbonylation followed by stabilization of the parameters at the levels slightly exceeding the control values by day 5.

[Unfavorable effect of the period of new year holidays on total and cardiovascular mortality].

We studied effect of the period of new year holydays on parameters of total and cardiovascular mortality. We analyzed all registered deaths in Kemerovo from 01.01.1998 to 31.12.2001 using data of the Kemerovo registry office (ZAGS). In time interval from 21 December to January 10 of each year there were 1830 deaths (968 men and 862 women). Between January 1 and 5 compared with preceding and subsequent days total and cardiovascular mortality were 27% (p = 0.003) and 25% (p = 0.024), high respectively. We also noted dependence of the parameter studied on age and sex. Thus the period of New Year holydays is associated with increased total and cardiovascular mortality and this association can not be explained by the effect of low temperatures.

[The peculiarities of immune status of patients with coronary heart disease in the predoperative period during the individual year].

The aim of the study was to evaluate the influence of the individual annual cycle (IAC), a period from one birthday to another, on the immune status of patients with coronary heart disease (CHD). The subjects, 146 male CHD patients, were hospitalized to undergo coronary bypass surgery (CBS). The preoperative period in patients who were operated on in the 4th IAC trimester was characterized by significant changes in cell immunity parameters, such as a decrease in the relative number of T-lymphocytes, T-helpers, and NK cells, which was associated with a significant increase in the frequency of pyoseptic complications after CBS. During the 1st IAC trimester there was an increase in the functional activity of polymorphonuclear leucocytes and monocyte level elevation, which corresponded with an increase in the number of coronarogenic complications. The data obtained demonstrate the dependence of the immune index dynamics of CHD patients on their IAC.

[Biorthythmological risk factors for progression of coronary heart disease]. / Bioritmologicheskie faktory riska progressirovaniia ishemicheskoi bolezni serdtsa.

The study was undertaken to study a role of periods of an individual annual cycle (IAC) (from the date of a birthday to the following one) and the season of a year in patients with coronary heart disease (CHD) in its progression. The study enrolled 52 patients with functional classes II and stable angina pectoris. Clinical and functional parameters were estimated by the data of bicycle ergometric test (BET), 24-hour monitoring (24-h M), ECG, and echocardiography. The vast majority of cases of progression of angina were observed in autumn and winter. Moreover, in patients with angina, the duration of silent ischemia significantly increased and heart rate variability decreased in winter and that of painful ischemia increased in autumn, as evidenced by 24-h M. Trimesters 1 (months 1 to 3 from the date of birth) and particularly IV (months 10 to 12 from the date of birth) have been found to be characterized by a poor course of CHD. Thus, in trimester IV, the duration of total, and silent ischemia significantly prolonged, as shown by 24-h M; episodes of latent coronary insufficiency increased in number, as evidenced by BET. Thus, the course of CHD is determined by a number of biorhythmological factors: the season of a year and the period of IAC.

[Connection between the season of year, season of birth, and trimester of the individual year in patients with ischemic heart disease at risk for myocardial infarction and its complications]. / Sviaz' sezona goda, sezona rozhdeniia i trimestrov individual'nogo goda bol'nykh ishemicheskoi bolezn'iu serdtsa s riskom razvitiia infarkta miokarda i ego oslozhnenii.

AIM: To clarify whether the periods of the individual year (from one birthday to another), season of the year, season of birth of patients with ischemic heart disease (IHD) are involved in development of myocardial infarction and its complications. MATERIAL AND METHODS: Case histories of 349 men hospitalized for acute myocardial infarction (MI) have been analysed. The analysis concerned the rate of MI onset in various seasons of the year, trimesters of the individual year (IY) and seasons of the patient's birth. RESULTS: Incidence of MI was higher in winter and summer. MI complications develop more frequently in spring. Trimester I and IV (months i-III and IX-XII since birthday) appeared more favourable for development of MI and its complications. Most favourable for IM was trimester III of IY, for MI complications--trimester II of IY. IM occurred more frequently in IHD patients born in autumn, complications in those born in summer. CONCLUSION: Development of MI and its complications is determined by a number of chronobiological factors: season of the year, season of the patient's birth and period of the individual year. This information is valuable in planning preventive measures.

[Immunoenzyme analysis of adenovirus hexon. Determination of antibodies from hyperimmunized chickens]. / Immunfermentnyi analiz geksona adenovirusov. Opredelenie antitel ot giperimmunizirovannykh kur.

We have elaborated three systems of enzyme-linked immunosorbent assay (ELISA) for detection of chicken IgG antibodies specific for hexon antigens of three immunologically distinct adenovirus groups: those of mammalian adenoviruses (Mastadenovira), typical avian adenoviruses (Aviadenovira) and of egg-drop syndrome-76 (EDS-76) virus. In each system the antibodies against respective hexons were specifically detected. In mammalian adenovirus hexons the ELISA detects primarily the type-specific (epsilon) and genus-specific (alpha) antigenic determinants. The time course of anti-hexon antibodies content was followed during immunization. The level of anti-hexon antibodies in egg yolk reflects adequately their content in blood serum. The technique is suitable for serological diagnosis of chicken adenoviral infections as well as for characterization of egg-yolk antibodies obtained by preparative hyperimmunization of hens.