Phycoremediation and biomass production from high strong swine wastewater for biogas generation improvement: An integrated bioprocess.

This study investigated the phycoremediation process from swine digestate integrated with photosynthetic biomass and biogas production in the context of circular economy. Effects of total ammonia nitrogen (TAN) and pH on biomass productivity and nutrients removal, using a central rotational composite design, were evaluated. pH showed a significant effect on biomass productivity and phosphate removal. The strain Chlorella sorokiniana (LBA#39) was able to tolerate up to 1300 mg TAN L-1 at neutral pH, with maximum biomass productivity of 198 mg DW L-1 d-1 and removal of 90 and 70 (%) of phosphate and nitrogen, respectively. The biomass harvested after phycoremediation from digestate showed high content of volatile solids (95.4%) and proteins (59.5%). Biochemical methane potential (BMP) from microalgae monodigestion was 292 ± 10 mLNCH4 gVSadd-1. The use microalgae biomass addition in the biodigestion process increased up to 32.1% in biogas production. It is an attractive approach to integrating raw materials into existing agro-industrial facilities and improving biogas production, adopting the concept of circular economy and mitigating greenhouse gas emissions.

Enteric viruses in lentic and lotic freshwater habitats from Brazil's Midwest and South regions in the Guarani Aquifer area.

The present study reports the monitoring of viruses indicating fecal contamination in two distinct regions affected by poor management of wastewater located above the Guarani Aquifer, which is one of the biggest freshwater reservoirs in the world. In the city of Três Lagoas (located in the Midwest region, in the state of Mato Grosso do Sul), water samples were collected from Lagoa Maior, a lake used for recreation, and in Concórdia (located in the South region, in the state of Santa Catarina), from the Queimados River, which crosses the urban area. Four sampling sites were monitored from March to July 2018 in Lagoa Maior, and four sampling sites were monitored along the urban part of the Queimados River area over two periods (rainy and dry). Water samples were analyzed by concentration of Human adenovirus (HAdV), Norovirus (NoV), Rotavirus A (RAV), and Hepatitis A virus (HAV) for the Lagoa Maior samples and RVA, HAV, and Porcine circovirus 2 (PCV2) for the Queimados River samples. All sampling sites presented enteric viruses, demonstrating fecal input and potential contamination of groundwater. Results highlight the need for wastewater management to improve environmental health quality.