Effects of marine sediment as agricultural substrate on soil microbial diversity: an amplicon sequencing study.

BACKGROUND: The soil microbiota has a direct impact on plant development and other metabolic systems, such as the degradation of organic matter and the availability of microelements and metabolites. In the context of agricultural soils, microbial activity is crucial for maintaining soil health and productivity. Thus, the present study aimed to identify, characterize, and quantify the microbial communities of four types of substrates with varying proportions of marine port sediment used for cultivating lemons. By investigating microbial diversity and relative abundance, the work aimed to highlight the importance of soil microbial communities in agriculture when alternative culture media was used. RESULTS: The composition and structure of the sampled microbial communities were assessed through the amplification and sequencing of the V3-V4 variable regions of the 16 S rRNA gene The results revealed a diverse microbial community composition in all substrate samples, with a total of 41 phyla, 113 classes, 266 orders, 405 families, 715 genera, and 1513 species identified. Among these, Proteobacteria, Bacteroidota, Planctomycetota, Patescibacteria, Chloroflexi, Actinobacteriota, Acidobacteriota, Verrucomicrobiota, and Gemmatimonadota accounted for over 90% of the bacterial reads, indicating their dominance in the substrates. CONCLUSIONS: The impact of the substrate origin on the diversity and relative abundace of the microbiota was confirmed. The higher content of beneficial bacterial communities for plant development identified in peat could explain why is considered an ideal agricultural substrate. Development of "beneficial for plants" bacterial communities in alternative agricultural substrates, regardless of the edaphic characteristics, opens the possibility of studying the forced and specific inoculation of these culture media aiming to be agriculturally ideals.

Metabolomic Approach to Study the 'Purple Queen' Pomegranate Cultivar Response to Alternative Culture Media and Phenological Stages.

The increasingly evident threat of depletion of world peat bogs is encouraging the search for and study of alternative agricultural substrates that can fully or partially replace peat, guaranteeing food supply (quality and quantity). On the other hand, the identification of the potential for the reuse of waste from relevant economic activities has increased in recent years, mainly motivated by the change to a sustainable circular economy, as is the case of port sediments. Taking into account that significant volumes of dredged port sediments are generated annually so that ports can maintain their economic activity, it is necessary to find objective, sustainable and safe reuse alternatives. In this sense, the objective of this study was to study the response of the "Purple Queen" pomegranate when grown with dredged port sediment. For this, the fruit production (kg), number of fruits (fruits tree-1), fruit weight (g), and seed yield (%) aiming to verify the correct tree development were evaluated. In addition, a 1H-NMR foliar metabolomic study for the three most relevant phenological phases was performed (flowering, fruit development, and post-harvest) to identify metabolic changes in trees. In total, 29 metabolites were identified; among them, 11 were amino acids, 6 organic acids, 5 sugars, and 7 secondary metabolites. The good agronomical development of the trees and fruits indicated the potential for using the dredged sediment as an agricultural substrate. On the other hand, the results revealed that the greatest variability in the metabolomic study occurred between the phenological phases and a lower variability is explained by the substrates used.

Life Cycle Assessment (LCA) of Substrate Mixes Containing Port Sediments for Sustainable 'Verna' Lemon Production.

The increase in maritime trade and its global economic importance have forced port management actors to carry out the periodic dredging of their sediments to maintain an adequate depth for the passage of large ships to maintain their operation and competitiveness. During the dredging process, large volumes of port sediment are generated. Dredged port sediment is currently considered a waste material and its disposal is regulated. Finding ways to safely reuse port sediments is necessary for sustainable development. In this study, a life cycle assessment (LCA) methodology was applied to identify the environmental impact of port sediments when used as a culture medium for lemon trees. A total of 90 lemon trees (Citrus limon L. Burm var 'Verna') were used in the trial. The trees were grown under controlled conditions using three substrates, with different portions of peat and port sediment (25%, 50%, and 75%) to identify the real impacts of the culture media on the growth process. The LCA was calculated and analyzed according to the ISO 14040:2006 standard, using the SimaPro v. 9.3 software (PRé Sustainability B.V, Amersfoort, The Netherlands). The functional unit defined for the three-culture media was 1 kg of lemons. The LCA results showed a significant increase in the environmental impact of lemon cultivation proportional to port sediment content (75%), due to the decrease in fruit production caused by the sediment. However, the least impact was identified for the culture medium at 50% peat and 50% port sediment. The greatest impacts were more related to crop management rather than the port sediment content. The results showed that the use of the port sediment, mixed with other substrates as an agricultural medium amendment, is a viable option for lemon growers.

Physico-Chemical Attributes of Lemon Fruits as Affected by Growing Substrate and Rootstock.

Due to its high content of bioactive compounds, the lemon is considered one of the most relevant species around the world. Its great economic importance is motivated, in addition to its fresh consumption, by its applications in the medical, pharmaceutical, and food industries, etc. However, the chemical and nutritional composition of lemon is not constant and can be influenced by external factors such as variety, weather conditions, crop management, etc. Determining the compositional variations of the fruit, essential to defining its potential use, was the main objective of this study. The physicochemical characteristics of the 'Verna' lemon were studied as a function of two controlled variables, the growing substrate and the rootstock. For this, 90 lemon trees were cultivated in three rootstocks and three different culture media. Lemon trees cultivated with 50% sediment/peat mix substrate presented a higher total production (590 lemons and 90.53 kg) while this production was 80% lower on trees cultivated with 75% marine sediment. Citrus macrophylla and Citrus aurantium/Citrus sinensis rootstocks showed a significantly higher production than the Citrus aurantium. All the fruits presented a predominantly yellow color appropriate for the market (0 < CI < +5). Nutritional and chemical parameters were consistent with data reported for the 'Verna' clones. All the obtained lemons were suitable for marketing and consumption both in fresh and processed forms. The results indicated the limited influence that the studied variables have on the quality parameters of lemon fruits, but they also could confirm the potential of marine sediment as a culture substrate.

Using Vis-NIR Spectroscopy for Predicting Quality Compounds in Foods.

Over the past four decades, near-infrared reflectance spectroscopy (NIRS) has become one of the most attractive and used technique for analysis as it allows for fast and simultaneous qualitative and quantitative characterization of a wide variety of food samples [...].

In Vivo and In Vitro Assays Evaluating the Biological Activity of Taurine, Glucose and Energetic Beverages.

Taurine is one of the main ingredients used in energy drinks which are highly consumed in adolescents for their sugary taste and stimulating effect. With energy drinks becoming a worldwide phenomenon, the biological effects of these beverages must be evaluated in order to fully comprehend the potential impact of these products on the health due to the fact nutrition is closely related to science since the population consumes food to prevent certain diseases. Therefore, the aim of this study was to evaluate the biological effects of taurine, glucose, classic Red Bull® and sugar-free Red Bull® in order to check the food safety and the nutraceutical potential of these compounds, characterising different endpoints: (i) Toxicology, antitoxicology, genotoxicology and life expectancy assays were performed in the Drosophila melanogaster model organism; (ii) The in vitro chemopreventive activity of testing compounds was determined by assessing their cytotoxicity, the proapoptotic DNA-damage capability to induce internucleosomal fragmentation, the strand breaks activity and the modulator role on the methylation status of genomic repetitive sequences of HL-60 promyelocytic cells. Whereas none tested compounds showed toxic or genotoxic effect, all tested compounds exerted antitoxic and antigenotoxic activity in Drosophila. Glucose, classic Red Bull® and sugar-free Red Bull® were cytotoxic in HL-60 cell line. Classic Red Bull® induced DNA internucleosomal fragmentation although none of them exhibited DNA damage on human leukaemia cells. In conclusion, the tested compounds are safe on Drosophila melanogaster and classic Red Bull® could overall possess nutraceutical potential in the in vivo and in vitro model used in this study. Besides, taurine could holistically be one of the bioactive compounds responsible for the biological activity of classic Red Bull®.

Potential of dredged bioremediated marine sediment for strawberry cultivation.

For the maintenance of the economic activity of the ports, it is necessary to dredge the marine sediments in order to guarantee their depth. These sediments, considered by European legislation as residues, present relevant limitations of use and generate environmental and economic problems concerning their final disposal. In this context, the present work aims to identify the phytoremediated dredged sediments potential as an alternative to the traditional substrate (peat) in horticultural growing through two-years controlled strawberry cultivation. The growing media mixes used were: (1) 100% peat (Pt) as a control substrate; (2) 100% dredged remediated sediment (DRS); (3) 50% each (Pt-DRS). The dredged sediment, plant drainage and strawberry plant parts (leaves, stems, roots, and fruits) were analyzed to mineral elements, heavy metal contents, and pesticide residues (polycyclic aromatic hydrocarbons, polychlorinated biphenyls and specific fumigants) during the experimental period. Only seven (Mn, Fe, Zn, Mo, Al, Mn and Ni) of the twenty-two metals and two (nitrates and fluorene) of the six hundred-thirteen pesticides analyzed were detected in the strawberry fruits. In all the cases, values detected were under the Spanish and European legal limit. The suitability of strawberry fruits for fresh and/or processed consumption with no risk was confirmed. Based on the results, can be affirmed that the dredged remediated sediment can be used as a culture substrate, alone or mixed with other substrates. Additional researches should be carried out to confirm the sediment characteristics and compare with other substrates to improve the physical and chemical properties.

The Health Benefits of Fruits and Vegetables.

We edited this Special Issue with the objective of bringing forth new data on the phytochemicals from vegetables and fruits, which are recommended for their health-promoting properties. Epidemiological, toxicological and nutritional studies suggested an association between fruit and vegetable consumption and lower incidence of chronic diseases, such as coronary heart problems, cancer, diabetes, and Alzheimer's disease. In this Special Issue, the protective roles (antioxidant and others bioactivities), new sustainable approaches to determine the quality, and the processing techniques that can modify the initial nutritional and antioxidant content of fruits, vegetables and additives have been addressed.

Food Safety and Nutraceutical Potential of Caramel Colour Class IV Using In Vivo and In Vitro Assays.

Nutraceutical activity of food is analysed to promote the healthy characteristics of diet where additives are highly used. Caramel is one of the most worldwide consumed additives and it is produced by heating natural carbohydrates. The aim of this study was to evaluate the food safety and the possible nutraceutical potential of caramel colour class IV (CAR). For this purpose, in vivo toxicity/antitoxicity, genotoxicity/antigenotoxicity and longevity assays were performed using the Drosophila melanogaster model. In addition, cytotoxicity, internucleosomal DNA fragmentation, single cell gel electrophoresis and methylation status assays were conducted in the in vitro HL-60 human leukaemia cell line. Our results reported that CAR was neither toxic nor genotoxic and showed antigenotoxic effects in Drosophila. Furthermore, CAR induced cytotoxicity and hipomethylated sat-α repetitive element using HL-60 cell line. In conclusion, the food safety of CAR was demonstrated, since Lethal Dose 50 (LD50) was not reached in toxicity assay and any of the tested concentrations induced mutation rates higher than that of the concurrent control in D. melanogaster. On the other hand, CAR protected DNA from oxidative stress provided by hydrogen peroxide in Drosophila. Moreover, CAR showed chemopreventive activity and modified the methylation status of HL-60 cell line. Nevertheless, much more information about the mechanisms of gene therapies related to epigenetic modulation by food is necessary.

Inhibition effect of polyurethane foam waste in dioxin formation.

The present work has been focused on studying the polychlorinated polychlorodibenzo-p-dioxin/furan (PCDD/F) inhibition in the combustion process by prior addition of an inhibitor to the fuel. Three different experiments of combustion were carried out at 850 °C in a laboratory-scale horizontal tubular quartz reactor, and several compounds were analyzed from the flue gas obtained, such as PCDD/Fs, dioxin-like polychlorinated biphenyls (dl-PCBs), NH3, HCN, NOx, HCl, Cl2, chlorobenzenes (ClBzs), chlorophenols (ClPhs), polycyclic aromatic hydrocarbons (PAHs), volatile and other semivolatile organic compounds. The fuel used was a synthetic waste which was composed of sawdust and PVC (95 wt% and 5 wt%, respectively) and the inhibitors studied were polyurethane foam (PUF) that can be found in mattress waste and gases from the oxidative pyrolysis of PUF. Due to the high N content of PUF, a significant decrease of PCDD/F and dl-PCB formation has been obtained in the experimental runs carried out with the inhibitors studied, particularly with the gases from the oxidative pyrolysis of PUF. In addition, it must be noted that emissions of incomplete combustion products have also decreased.

Toxicological Studies of Czech Beers and Their Constituents.

BACKGROUND: Czech beers are unique because they are brewed using specific technology at a particular latitude and for being entirely produced in the area of the Czech Republic. The purpose of this work is the evaluation of toxicological effects of a variety of freeze-dried Czech beers, their raw materials (malts, hops and yeast) and processed-beer (wort, hopped wort and young beer). METHODS: In vivo assays to evaluate the safety and protective effects in the Drosophila melanogaster eukaryotic system, and the in vitro evaluations of chemopreventive and DNA damage activity using the HL-60 tumour human cell line were carried out. RESULTS: The safe effects for all the analysed substances and general protective effects against H2O2 were shown both at the individual and genomic level in the Drosophila animal model, with some exceptions. Moreover, all the substances were able to inhibit the tumour cell growth and to induce DNA damage in the HL-60 cells at different levels (proapoptotic, single/double strands breaks and methylation status). CONCLUSIONS: The promising effects shown by freeze-dried Czech beers due to their safety, protection against a toxin, chemopreventive potential and the induction of DNA damage in tumour cells, allow the proposition of Czech beer as a beverage with nutraceutic potential.

Physicochemical Characterization and Biological Activities of Black and White Garlic: In Vivo and In Vitro Assays.

White and three types of black garlic (13, 32, and 45 days of aging, named 0C1, 1C2, and 2C1, respectively) were selected to study possible differences in their nutraceutic potential. For this purpose, garlic were physicochemically characterized (Brix, pH, aW, L, polyphenol, and antioxidant capacity), and both in vivo and in vitro assays were carried out. Black garlic samples showed higher polyphenol content and antioxidant capacity than the white ones. The biological assays showed that none of the samples (neither raw nor black garlic) produced toxic effects in the Drosophila melanogaster animal genetic model, nor exerted protective effects against H2O2, with the exception of the 0C1 black garlic. Moreover, only white garlic was genotoxic at the highest concentration. On the other hand, 0C1 black garlic was the most antigenotoxic substance. The in vivo longevity assays showed significant extension of lifespan at some concentrations of white and 0C1and 1C2 black garlic. The in vitro experiments showed that all of the garlic samples induced a decrease in leukemia cell growth. However, no type of garlic was able to induce proapoptotic internucleosomal DNA fragmentation. Taking into account the physicochemical and biological data, black garlic could be considered a potential functional food and used in the preventive treatment of age-related diseases. In addition, our findings could be relevant for black-garlic-processing agrifood companies, as the economical and timing costs can significantly be shortened from 45 to 13 days of aging.

Prediction of Agro-Morphological and Nutritional Traits in Ethiopian Mustard Leaves (Brassica Carinata A. Braun) by Visible-Near-Infrared Spectroscopy.

The particular characteristics of some of the Ethiopian mustard accessions available from seed banks could be used to increase the production and the diversity of products available to consumers and to improve their general quality. The objectives of this study were to determine the genetic variability for agro-morphological (days to first flowering: DFF and leaf pubescence: LP) and nutritional traits (total phenolic content: TPC) among accessions, and to evaluate the potential of near-infrared spectroscopy (NIRS) to predict these traits in Ethiopian mustard leaves. A great variation was found for the traits evaluated. The reference values were regressed against different spectral transformations by modified partial least-squares (MPLS) regression. The coefficients of determination in cross-validation (R²cv) shown by the equations for DFF, LP and TPC were 0.95, 0.63 and 0.99, respectively. The standard deviation to standard error of cross-validation ratio (RPD), were for these traits, as follows: DFF: 4.52, LP: 1.53 and, TPC: 24.50. These results show that the equations developed for DFF and TPC in Ethiopian mustard, can be predicted with sufficient accuracy for screening purposes and quality control, respectively. In addition, the LP equation can be used to identify those samples with "low", "medium" and "high" groups. From the study of the mean and deviation standard spectra, and regression vectors of MPLS models it can be concluded that some major cell components, highly participated in modelling the equations for these traits.

Quantification of Total Phenolic and Carotenoid Content in Blackberries (Rubus Fructicosus L.) Using Near Infrared Spectroscopy (NIRS) and Multivariate Analysis.

A rapid method to quantify the total phenolic content (TPC) and total carotenoid content (TCC) in blackberries using near infrared spectroscopy (NIRS) was carried out aiming to provide reductions in analysis time and cost for the food industry. A total of 106 samples were analysed using the Folin-Ciocalteu method for TPC and a method based on Ultraviolet-Visible Spectrometer for TCC. The average contents found for TPC and TCC were 24.27 mg·g-1 dw and 8.30 µg·g-1 dw, respectively. Modified partial least squares (MPLS) regression was used for obtaining the calibration models of these compounds. The RPD (ratio of the standard deviation of the reference data to the standard error of prediction (SEP)) values from external validation for both TPC and TCC were between 1.5 < RPDp < 2.5 and RER values (ratio of the range in the reference data to SEP) were 5.92 for TPC and 8.63 for TCC. These values showed that both equations were suitable for screening purposes. MPLS loading plots showed a high contribution of sugars, chlorophyll, lipids and cellulose in the modelling of prediction equations.

Characterisation and prediction of carbohydrate content in zucchini fruit using near infrared spectroscopy.

BACKGROUND: Zucchini fruit plays an important part in healthy nutrition due to its high content of carbohydrates. Recent studies have demonstrated the feasibility of visible-NIRS to predict quality profile. However, this procedure has not been applied to determine carbohydrates. RESULTS: Visible-NIR and wet chemical methods were used to determine individual sugars and starch in zucchini fruits. By applying a principal component analysis (PCA) with NIR spectral data a differentiation between the less sweet versus the sweetest zucchini accessions could be found. For the determination of carbohydrate content effective prediction models for individual sugars such as glucose, fructose, sucrose and starch by using partial least squares (PLS) regression have been developed. CONCLUSION: The coefficients of determination in the external validation (R2 VAL) ranged from 0.66 to 0.85. The standard deviation (SD) to standard error of prediction ratio (RPD) and SD to range (RER) were variable for different quality compounds and showed values that were characteristic of equations suitable for screening purposes. From the study of the MPLS loadings of the first three terms of the different equations for sugars and starch, it can be concluded that some major cell components such as pigments, cellulose, organic acids highly participated in modelling the equations for carbohydrates. © 2017 Society of Chemical Industry.

PCDD/F determination in sewage sludge composting. Influence of aeration and the presence of PCP.

Composting of sewage sludge is a common practice for sludge disposal. Some previous studies found high levels of polychorodibenzo-p-dioxins and polychorodibenzofurans (PCDD/Fs) after composting, especially octachlorodibenzo-p-dioxin (OCDD) but also 1234678-heptachlorodibenzo-p-dioxin (1234678-HpCDD) to a lesser extent. In this work, the concentrations of OCDD, 1234678-HpCDD and the rest of the 17 toxic congeners of PCDD/Fs were determined in compost obtained under different conditions. Although the toxicity of the two compounds mentioned above is small, their generation may reach undesirable levels. The PCDD/F content was analyzed in a composting plant and in a laboratory test. In both cases, the composted material was a mixture of sewage sludge, straw and sawdust. The composting plant was a tunnel with air turbine aeration and with a turner to homogenize and move the mixture upwards. The laboratory tests were carried out with Dewar vessels (with air dispersion at the bottom and controlled temperature) and with small vessels inside a controlled oven with non-forced aeration. The laboratory runs were also carried out with the addition of pentachlorophenol in some runs, as a dioxin precursor. The highest OCDD levels were found in three samples of the composting plant (30000-90000pg/g dry matter or dm), with toxicity values surpassing the limit level for soil amendment (17pgI-TEQ/gdm). Their formation was analyzed considering their concentration vs. that of octachorodibenzofuran (OCDF), which is not formed during composting. In the laboratory, in experiments carried out in a vessel with non-forced aeration conditions and with the addition of pentachlorophenol, the formation of OCDD was significant (e.g. from 80 to 1500pg/gdm). That means that these two factors, non-forced aeration and the presence of pentachlorophenol, can cause the OCDD formation.

Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Anti-Genotoxicity, Cytotoxicity and Apoptotic Effects.

Zucchini (Cucurbita pepo subsp. pepo) is a seasonal vegetable with high nutritional and medical values. Many useful properties of this fruit are attributed to bioactive compounds. Zucchini fruits ("Yellow" and "Light Green" varieties) and four distinctive components (lutein, ß-carotene, zeaxanthin and dehydroascorbic acid) were selected. Firstly, the lutein, ß-carotene, zeaxanthin and dehydroascorbic acid contents were determined in these fruits. Then, in order to evaluate the safety and suitability of their use, different assays were carried out: (i) genotoxicity and anti-genotoxicity tests to determine the safety and DNA-protection against hydrogen peroxide; (ii) cytotoxicity; and (iii) DNA fragmentation and Annexin V/PI (Propidium Iodide) assays to evaluate the pro-apoptotic effect. Results showed that: (i) all the substances were non-genotoxic; (ii) all the substances were anti-genotoxic except the highest concentration of lutein; (iii) "Yellow" zucchini epicarp and mesocarp exhibited the highest cytotoxic activity (IC50 > 0.1 mg/mL and 0.2 mg/mL, respectively); and (iv) "Light Green" zucchini skin induced internucleosomal DNA fragmentation, ß-carotene being the possible molecule responsible for its pro-apoptotic activity. To sum up, zucchini fruit could play a positive role in human health and nutrition due to this fruit and its components were safe, able to inhibit significantly the H2O2-induced damage and exhibit anti-proliferative and pro-apoptotic activities toward HL60 (human promyelocytic leukemia cells) tumor cells. The information generated from this research should be considered when selecting potential accessions for breeding program purposes.

Rapid and Cost-Effective Quantification of Glucosinolates and Total Phenolic Content in Rocket Leaves by Visible/Near-Infrared Spectroscopy.

The potential of visible-near infrared spectroscopy to predict glucosinolates and total phenolic content in rocket (Eruca vesicaria) leaves has been evaluated. Accessions of the E. vesicaria species were scanned by NIRS as ground leaf, and their reference values regressed against different spectral transformations by modified partial least squares (MPLS) regression. The coefficients of determination in the external validation (R²VAL) for the different quality components analyzed in rocket ranged from 0.59 to 0.84, which characterize those equations as having from good to excellent quantitative information. These results show that the total glucosinolates, glucosativin and glucoerucin equations obtained, can be used to identify those samples with low and high contents. The glucoraphanin equation obtained can be used for rough predictions of samples and in case of total phenolic content, the equation showed good correlation. The standard deviation (SD) to standard error of prediction ratio (RPD) and SD to range (RER) were variable for the different quality compounds and showed values that were characteristic of equations suitable for screening purposes or to perform accurate analyses. From the study of the MPLS loadings of the first three terms of the different equations, it can be concluded that some major cell components such as protein and cellulose, highly participated in modelling the equations for glucosinolates.

Isocyanate emissions from pyrolysis of mattresses containing polyurethane foam.

This study examined the emissions of powerful asthmatic agents called isocyanates from small-scale pyrolysis experiments of two common foams employed in mattress production such as flexible polyurethane foam (FPUF) and viscoelastic memory foam (VMF). A nitrogen atmosphere and five different temperatures, 300, 350, 400, 450 and 850 °C, were selected to carry out the experiments in order to evaluate the worst possible conditions for thermal degradation. A similar trend for both materials was found. At lower temperatures, diisocyanates were the most important products whereas at 850 °C monoisocyanates, and mainly isocyanic acid released mainly from the thermal cracking of diisocyanates evolved directly from the polymer chains. The total yields of isocyanates were in the range of 1.43-11.95 mg/m3 for FPUF at 300-850 °C and 0.05-6.13 mg/m3 for VMF, 300-850 °C. This difference could be a consequence of the lower amount of isocyanates employed in the VMF production which was confirmed by the nitrogen content of the foams, 5.95% FPUF vs. 3.34% in VMF. Additionally, a qualitative search for so far unknown isocyanates was performed in samples from the pyrolysis of FPUF at 300, 400 and 850 °C. It was confirmed that six different aminoisocyanates at 300 °C were evolved, whereas at 400 and 850 °C only five of them were detected. The general trend observed was a decrease of the aminoisocyanate levels with increasing pyrolysis temperature.

Pollutant emissions from the pyrolysis and combustion of viscoelastic memory foam.

Thermal degradation of viscoelastic memory foam (VMF) in a horizontal laboratory scale reactor has been studied. Pyrolysis and combustion experiments under sub-stoichiometric conditions were performed at four different temperatures (550°C, 650°C, 750°C and 850°C) for the determination of pollutants. Analyses of gas and semivolatile compounds, including polychlorodibenzo-p-dioxins and furans (PCDD/Fs) and dioxin-like polychlorobiphenyls (dl-PCBs) are shown. From the results, it was deduced that pyrolytic conditions favor the formation of PAHs, methane, ethylene, NH3 and dl-PCBs, whereas the presence of oxygen involves a higher emission of PCDD/Fs and simple N-containing compounds such as NO and HCN. The toxic levels calculated for PAHs, PCDD/Fs and dl-PCBs in all cases were low confirming that the incineration of VMF mattress waste could be a good option for waste management. Nevertheless, relatively high emissions of NO, NH3 and HCN were obtained and their reduction must be considered.