Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
J Mol Biol ; 433(6): 166659, 2021 03 19.
Article in English | MEDLINE | ID: mdl-33010306

ABSTRACT

Epigenetic modifications and nucleosome positioning play an important role in modulating gene expression. However, how the patterns of epigenetic modifications and nucleosome positioning are established around promoters is not well understood. Here, we have addressed these questions in a series of genome-wide experiments coupled to a novel bioinformatic analysis approach. Our data reveal a clear correlation between CpG density, promoter activity and accumulation of active or repressive histone marks. CGI boundaries define the chromatin promoter regions that will be epigenetically modified. CpG-rich promoters are targeted by histone modifications and histone variants, while CpG-poor promoters are regulated by DNA methylation. CGIs boundaries, but not transcriptional activity, are essential determinants of H2A.Z positioning in vicinity of the promoters, suggesting that the presence of H2A.Z is not related to transcriptional control. Accordingly, H2A.Z depletion has no impact on gene expression of arrested mouse embryonic fibroblasts. Therefore, the underlying DNA sequence, the promoter CpG density and, to a lesser extent, transcriptional activity, are key factors implicated in promoter chromatin architecture.


Subject(s)
CpG Islands , Epigenesis, Genetic , Epigenome , Histones/genetics , Promoter Regions, Genetic , Protein Processing, Post-Translational , Animals , Chromatin/metabolism , Chromatin/ultrastructure , Chromatin Assembly and Disassembly , Computational Biology/methods , DNA Methylation , Embryo, Mammalian , Fibroblasts/cytology , Fibroblasts/metabolism , Histones/chemistry , Histones/deficiency , Histones/metabolism , Mice , Mice, Knockout , Primary Cell Culture , Protein Isoforms/chemistry , Protein Isoforms/genetics , Protein Isoforms/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...