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1.
Behav Brain Res ; 313: 151-157, 2016 10 15.
Article in English | MEDLINE | ID: mdl-27424156

ABSTRACT

AIM: Many studies showed that visual stimuli are frequently experienced as shorter than equivalent auditory stimuli. These findings suggest that timing is distributed across many brain areas and that "different clocks" might be involved in temporal processing. The aim of this study is to investigate, with the application of tDCS over V1 and A1, the specific role of primary sensory cortices (either visual or auditory) in temporal processing. METHOD: Forty-eight University students were included in the study. Twenty-four participants were stimulated over A1 and 24 participants were stimulated over V1. Participants performed time bisection tasks, in the visual and the auditory modalities, involving standard durations lasting 300ms (short) and 900ms (long). RESULTS: When tDCS was delivered over A1, no effect of stimulation was observed on perceived duration but we observed higher temporal variability under anodic stimulation compared to sham and higher variability in the visual compared to the auditory modality. When tDCS was delivered over V1, an under-estimation of perceived duration and higher variability was observed in the visual compared to the auditory modality. CONCLUSION: Our results showed more variability of visual temporal processing under tDCS stimulation. These results suggest a modality independent role of A1 in temporal processing and a modality specific role of V1 in the processing of temporal intervals in the visual modality.


Subject(s)
Auditory Cortex/physiology , Time Perception/physiology , Visual Cortex/physiology , Acoustic Stimulation , Adult , Humans , Photic Stimulation , Transcranial Direct Current Stimulation , Young Adult
2.
Epilepsia ; 41(11): 1463-8, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11077461

ABSTRACT

PURPOSE: New post-resection spikes on electrocorticography (ECoG) after lesionectomy in patients with seizures may represent residual epileptogenic tissue or presumed reactive injury spikes. We investigated the existence of post-resection injury spikes by eliminating the possibility of residual epileptogenic tissue. METHODS: Preresection and post-resection ECoG was performed on seven patients with an intra-axial neocortical tumor (glioblastoma multiforme or metastasis) and no history of seizures. All tumors were gross-totally resected. RESULTS: The mean age of the patients was 59 years. The tumor location was frontal in four patients, parietal in two, and temporal in one. Two patients had preresection spikes with an average rate of 68 spikes/min that disappeared after surgery. Two different patients had new post-resection spikes, with an average firing rate of 4 spikes/min, despite normal preresection ECoG. In one of these patients, the new spikes were superimposed over a burst suppression pattern. Neither patient developed seizures after surgery. CONCLUSIONS: Surgical irritation of the neocortex is sufficient to produce reactive post-resection epileptogenic discharges surrounding an intra-axial neocortical tumor even in the absence of preoperative seizures and spikes. Injury spikes fire at a slow rate and are not predictive of clinical seizures.


Subject(s)
Brain Neoplasms/surgery , Cerebral Cortex/surgery , Electroencephalography/statistics & numerical data , Epilepsy/diagnosis , Glioblastoma/surgery , Postoperative Complications/diagnosis , Adult , Aged , Anticonvulsants/therapeutic use , Cerebral Cortex/physiopathology , Epilepsy/physiopathology , Epilepsy/prevention & control , Female , Humans , Male , Middle Aged , Postoperative Complications/physiopathology , Postoperative Complications/prevention & control , Prospective Studies
3.
J Clin Invest ; 106(4): 483-91, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10953023

ABSTRACT

Homocysteine is a risk factor for the development of atherosclerosis and its thrombotic complications. We have employed an animal model to explore the hypothesis that an increase in reactive oxygen species and a subsequent loss of nitric oxide bioactivity contribute to endothelial dysfunction in mild hyperhomocysteinemia. We examined endothelial function and in vivo oxidant burden in mice heterozygous for a deletion in the cystathionine beta-synthase (CBS) gene, by studying isolated, precontracted aortic rings and mesenteric arterioles in situ. CBS(-/+) mice demonstrated impaired acetylcholine-induced aortic relaxation and a paradoxical vasoconstriction of mesenteric microvessels in response to superfusion of methacholine and bradykinin. Cyclic GMP accumulation following acetylcholine treatment was also impaired in isolated aortic segments from CBS(-/+) mice, but aortic relaxation and mesenteric arteriolar dilation in response to sodium nitroprusside were similar to wild-type. Plasma levels of 8-epi-PGF(2alpha) (8-IP) were somewhat increased in CBS(-/+) mice, but liver levels of 8-IP and phospholipid hydroperoxides, another marker of oxidative stress, were normal. Aortic tissue from CBS(-/+) mice also demonstrated greater superoxide production and greater immunostaining for 3-nitrotyrosine, particularly on the endothelial surface. Importantly, endothelial dysfunction appears early in CBS(-/+) mice in the absence of structural arterial abnormalities. Hence, mild hyperhomocysteinemia due to reduced CBS expression impairs endothelium-dependent vasodilation, likely due to impaired nitric oxide bioactivity, and increased oxidative stress apparently contributes to inactivating nitric oxide in chronic, mild hyperhomocysteinemia.


Subject(s)
Endothelium, Vascular/physiopathology , Hyperhomocysteinemia/physiopathology , Acetylcholine/pharmacology , Animals , Aorta/drug effects , Aorta/pathology , Aorta/physiopathology , Arteriosclerosis/etiology , Cystathionine beta-Synthase/genetics , Cystathionine beta-Synthase/metabolism , Dinoprost/analogs & derivatives , Dinoprost/metabolism , Disease Models, Animal , F2-Isoprostanes , Heterozygote , Humans , Hyperhomocysteinemia/genetics , Hyperhomocysteinemia/pathology , In Vitro Techniques , Lipid Peroxides/metabolism , Mice , Mice, Mutant Strains , Nitroprusside/pharmacology , Reactive Oxygen Species/metabolism , Risk Factors , Thrombosis/etiology , Tyrosine/analogs & derivatives , Tyrosine/metabolism , Vasodilation/drug effects
4.
Curr Opin Cardiol ; 15(6): 409-15, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11198623

ABSTRACT

Endothelial dysfunction is an early and persistent vascular abnormality in the evolution of atherothrombotic disease. Risk factors for atherosclerosis promote an inflammatory oxidative environment in the vasculature that induces pathologic changes in endothelial function, including the support of enhanced smooth muscle tone, thrombosis, and smooth muscle proliferation. This article provides an overview of the molecular basis of endothelial dysfunction and of its diagnosis and treatment.


Subject(s)
Coronary Disease/physiopathology , Coronary Vessels/physiopathology , Endothelium, Vascular/physiopathology , Antioxidants/therapeutic use , Coronary Disease/prevention & control , Estrogen Replacement Therapy , Female , Humans , Lipid Peroxidation , Middle Aged , Oxidative Stress , Risk Factors
5.
Drug News Perspect ; 13(9): 523-9, 2000 Nov.
Article in English | MEDLINE | ID: mdl-12879123

ABSTRACT

The endothelial dysfunction associated with nitric oxide (NO) depletion accompanies the development of atherothrombotic disease. Recent evidence suggests that oxidative reactions in the vasculature promote atherothrombosis. Several risk factors for atherothrombosis, such as diabetes mellitus, cigarette smoking, hypertension, hyperhomocyst(e)inemia and hypercholesterolemia, are associated with oxidative reactions in the vasculature and with endothelial dysfunction. Traditional and novel treatments for atherothrombosis increase bioavailable NO and may decrease oxidant stress; however, the effect of this treatment on improving the long-term prognosis of patients with risk factors for atherothrombosis has yet to be determined.

6.
Electroencephalogr Clin Neurophysiol ; 94(5): 338-48, 1995 May.
Article in English | MEDLINE | ID: mdl-7774520

ABSTRACT

In standard EEG recordings, spikes appear as single events characterized mainly by the scalp location of the their peak voltage. The signal-to-noise ratio of raw EEG is usually too high to permit more detailed analysis. We used spike averaging to improve the resolution of interictal spikes in 40 patients with temporal lobe epilepsy. Spikes were identified visually in raw, digitally stored EEG. When multiple spike types were present in a patient, they were grouped separately. Spikes were synchronized for averaging by aligning their negative peaks in a designated channel. Sixteen patients demonstrated spike propagation from anterior temporal to posterior temporal electrode locations. Thirty-six patients demonstrated spread of spikes from anterior temporal to fronto-polar electrode sites. While anterior temporal and fronto-polar spikes were often synchronous, fronto-polar spikes followed anterior temporal discharges in 25% of cases and preceded them in 13%. Spike averaging revealed propagation patterns not apparent on visual inspection of raw EEG. We speculate that these patterns may reflect inherent physiological properties of temporal and frontal neuronal circuits, possibly utilized by the epileptogenic process.


Subject(s)
Electroencephalography , Epilepsy, Temporal Lobe/diagnosis , Epilepsy, Temporal Lobe/physiopathology , Adolescent , Adult , Aged , Brain Mapping , Female , Frontal Lobe/physiopathology , Humans , Male , Middle Aged , Signal Processing, Computer-Assisted , Temporal Lobe/physiopathology
7.
J Biol Chem ; 269(2): 903-9, 1994 Jan 14.
Article in English | MEDLINE | ID: mdl-7904604

ABSTRACT

v-erbA, a viral oncogenic homolog of thyroid hormone receptor (TR), blocks the effect of T3 in TR-mediated transcription. The mechanism(s) for this dominant negative effect by v-erbA on TRs is unknown but may involve competition between v-erbA and TR-containing complexes for binding to thyroid hormone response elements (TREs) and/or protein-protein interactions between v-erbA and TR. To investigate these potential mechanisms, we used the electrophoretic mobility shift assay to compare in vitro translated v-erbA and TR alpha binding to two TREs-chick lysozyme TRE (F2) and direct repeat TRE (DR4). v-erbA bound as a homodimer to these TREs, whereas TR alpha bound as a homodimer and monomer. T3 decreased TR alpha homodimer binding to the TREs as we reported previously; however, surprisingly, high concentrations of T3 (10(-6) M) also decreased v-erbA homodimer binding to the TREs. Additionally, v-erbA formed heterodimers with nuclear proteins such as retinoid X receptor and T3 receptor auxiliary protein as well as with TR alpha. These dimers remained bound to DNA in the presence of T3. Finally, v-erbA could not mediate ligand-dependent transcriptional activation even at 10(-6) M T3 but could block ligand-dependent TR-mediated transactivation in co-transfection experiments. v-erbA also exhibited differential dominant negative activity on F2 and DR4 suggesting that half-site sequence and/or orientation may influence v-erbA-dominant negative activity. In sum, there are multiple v-erbA complexes that bind to TREs in the presence of T3, which all may contribute to v-erbA's dominant negative effect on TR-mediated transcription by competing with TR-containing complexes for binding to TREs.


Subject(s)
Receptors, Thyroid Hormone/metabolism , Retroviridae Proteins, Oncogenic/chemistry , Base Sequence , DNA-Binding Proteins/chemistry , Genes, Dominant , Macromolecular Substances , Molecular Sequence Data , Oncogene Proteins v-erbA , Oncogenes , Protein Binding , Receptors, Thyroid Hormone/chemistry , Recombinant Proteins , Retroviridae Proteins, Oncogenic/metabolism , Triiodothyronine/metabolism
8.
Mol Cell Endocrinol ; 97(1-2): 93-9, 1993 Nov.
Article in English | MEDLINE | ID: mdl-7511545

ABSTRACT

There are multiple factors that potentially can induce structural changes in DNA-bound thyroid hormone receptors (TRs) including protein-protein interactions, ligand-binding to TRs, and the thyroid hormone response element (TRE) sequence. We used a battery of anti-TR antibodies that recognize the amino-terminal, hinge, or carboxy-terminal regions of TRs to study changes in the epitope regions of in vitro translated TRs in electrophoretic mobility shift assays. We found that the carboxy-terminal and hinge region antibodies recognized TR homodimers but not TR/T3-receptor auxiliary protein or TR/retinoid X receptor heterodimers. The amino-terminal antibodies detected conformational changes due to ligand binding. In contrast, each antibody recognized TR complexes bound to TREs containing half-sites arranged in three different orientations. These results suggest that dimerization with nuclear proteins and ligand-binding, rather than the orientation of TRE half-sites, cause changes in several TR subregions.


Subject(s)
Protein Conformation , Receptors, Thyroid Hormone/chemistry , Antibody Specificity , Binding Sites , DNA/metabolism , DNA, Complementary/genetics , Epitopes/immunology , Humans , Isoantibodies/immunology , Oligonucleotide Probes , Protein Binding , Receptors, Thyroid Hormone/immunology , Receptors, Thyroid Hormone/metabolism , Triiodothyronine/metabolism
9.
J Biol Chem ; 267(6): 3565-8, 1992 Feb 25.
Article in English | MEDLINE | ID: mdl-1740410

ABSTRACT

Thyroid hormone receptors (TRs) are ligand-dependent transcription factors that bind to thyroid hormone response elements (TREs) to mediate positive and negative regulation of transcription of thyroid hormone-responsive genes. TR binding to TREs can be enhanced by interaction with a nuclear protein, triiodothyronine (T3) receptor auxiliary protein (TRAP). There are two major isoforms of thyroid hormone receptors, TR alpha-1 and TR beta-1, which are encoded on two separate genes. We studied the binding of TR alpha-1 and TR beta-1 to several TREs: the chick lysozyme TRE (F2), which is positively regulated by T3; rabbit beta-myosin heavy chain TRE, which is negatively regulated by T3; and an idealized inverted palindrome, TRElap. We demonstrate the formation of homodimers, TR alpha/TR beta dimers, and TR/TRAP heterodimers when receptor is bound to these DNA sequences. Surprisingly, we found that T3 decreased TR alpha-1 and TR beta-1 homodimer binding in a dose-dependent manner to these TREs as well as TR alpha/TR beta dimer binding to F2. In contrast, T3 did not affect TR/TRAP heterodimer binding to TREs suggesting that this heterodimer may be the stable complex occupying TREs in the presence of ligand.


Subject(s)
DNA/metabolism , Receptors, Thyroid Hormone/metabolism , Triiodothyronine/metabolism , Animals , Cell Line , Electrophoresis , Gene Expression Regulation , Humans , Muramidase/metabolism , Myosins/metabolism , Protein Binding , Rats , Receptors, Thyroid Hormone/genetics , Transcription, Genetic
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