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Methods Mol Biol ; 1568: 355-365, 2017.
Article in English | MEDLINE | ID: mdl-28421511

ABSTRACT

This appendix describes the vitrification of blastocysts using VitriBlast™ (VBK) and ThermoBlast™ (TBK) from Nidacon, Mölndal, Sweden. The technique used and the reason for not including DMSO in the medium at the production stage, but including it separately in the kit, and the importance of collapsing the blastocyst prior to vitrification will be explained and described.


Subject(s)
Blastocyst , Cryopreservation/methods , Cryoprotective Agents , Vitrification , Animals , Blastocyst/cytology , Female , Humans
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