Subject(s)
Adenoma/pathology , Anus Diseases/pathology , Choristoma/pathology , Crohn Disease/diagnosis , Adenoma/diagnosis , Adenoma/surgery , Adult , Anus Diseases/diagnosis , Anus Diseases/surgery , Biopsy, Needle , Breast , Choristoma/diagnosis , Choristoma/surgery , Crohn Disease/therapy , Female , Follow-Up Studies , Humans , Immunohistochemistry , Pain/diagnosis , Pain/etiology , Rare Diseases , Risk Assessment , Treatment OutcomeSubject(s)
Antibodies, Monoclonal/administration & dosage , Antineoplastic Agents/administration & dosage , Breast Neoplasms/pathology , Carcinoma/drug therapy , Carcinoma/secondary , Meningeal Neoplasms/drug therapy , Meningeal Neoplasms/secondary , Meningitis/drug therapy , Meningitis/etiology , Antibodies, Monoclonal, Humanized , Carcinoma/diagnosis , Cerebral Ventricles , Fatal Outcome , Female , Humans , Injections, Intraventricular , Injections, Spinal , Magnetic Resonance Imaging , Meningeal Neoplasms/diagnosis , Meningitis/diagnosis , Middle Aged , TrastuzumabABSTRACT
The presentation of an adrenal cortical carcinoma with myxoid ground substance is an unusual and apparently rare event. To my knowledge, only one such case has been previously reported in the literature. A second case of a metastasizing adrenal cortical carcinoma with abundant acidic mucosubstances and a myxomalike appearance is reported herein. The myxoid variant of adrenal cortical carcinoma should be included in the differential diagnosis of retroperitoneal myxoid tumors.
Subject(s)
Adrenal Cortex Neoplasms/diagnosis , Myxoma/diagnosis , Adrenal Cortex Neoplasms/pathology , Diagnosis, Differential , Humans , Male , Middle Aged , Myxoma/pathology , Retroperitoneal Neoplasms/diagnosis , Retroperitoneal Neoplasms/pathologyABSTRACT
The authors report a case of Duchenne muscular dystrophy in which a deletion was determined by multiplex polymerase chain reaction using postmortem tissue from a proband who died in 1985, 2 years before the cloning of the dystrophin gene. Several extraction methods were analyzed to determine optimal conditions for recovery of DNA from fixed tissue. Variables examined were tissue lysis times and temperatures, a simple salting-out procedure for purification of DNA, polymerase chain reaction amplification of crude lysate versus purified DNA, and lysis of different tissues and tissue quantities. Extracted DNA was analyzed spectrophotometrically, electrophoretically, and by its suitability for polymerase chain reaction amplification using exon flanking primers of the dystrophin gene. Lysis at 37 degrees C for less than 24 hours led to the recovery of predominantly low-molecular-weight DNA, which was unsuitable for polymerase chain reaction in this experience. Lysis at 54 degrees C consistently yielded visible, spoolable quantities of high-molecular-weight DNA in as little as 24 hours. Direct amplification of crude, unpurified lysates was unsuccessful. However, purified samples yielded consistent amplification products. The purification step was simplified by substituting a rapid salting-out procedure for organic extractions.
