Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia.

The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.

Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia

The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.

Effects of feeding a multivalent polyclonal antibody preparation on feedlot performance, carcass characteristics, rumenitis, and blood gas profile in Bos indicus biotype yearling bulls.

The objective of this study was to evaluate effects of feeding monensin (MON) or a multivalent polyclonal antibody preparation (PAP) against several rumen microorganisms on feedlot performance, carcass characteristics, blood gas profile, and rumenitis of Bos indicus biotype (BT) yearling bulls. The study was designed as a completely randomized design with a 3 × 2 factorial arrangement, replicated 4 times, in which 32 yearling bulls of each of 3 BT evaluated (3-way-cross, TC; Canchim, CC; and Nellore, NE) were fed diets containing either MON at 300 mg·d(-1) or PAP at 10 mL·d(-1) across 3 different periods. No significant (P > 0.10) feed additive (FA) main effects were observed for any of the feedlot performance variables and carcass characteristics with the exception of dressing percentage. Yearling bulls receiving PAP had a decreased (P = 0.047) dressing percentage when compared with yearling bulls receiving MON. Significant (P < 0.05) BT main effects were observed for all feedlot performance variables and carcass characteristics with the exception of kidney-pelvic fat expressed in kilograms (P = 0.49) and LM lipids content (P = 0.45). Crossbred yearling bulls (TC and CC) had greater (P < 0.001) ADG, DMI in kilograms, DMI as % of BW, and improved (P = 0.001) G:F when compared with NE yearling bulls. A tendency (P = 0.072) for a FA main effect was observed for rumenitis scores, in which yearling bulls receiving PAP had lesser rumenitis scores than those receiving MON. When the data were disposed as frequency percentage, 55.6% and 45.7% of the rumens from yearling bulls fed PAP and MON were scored between 0 and 1, respectively (0 = no lesions, 10 = severe lesions). Likewise, a significant BT main effect was observed (P = 0.008), where NE yearling bulls had greater rumenitis scores than those of crossbred yearling bulls (TC and CC). No significant FA main effects were observed (P > 0.10) for any of the fatty acids measured in the subcutaneous adipose tissue, with the exception that yearling bulls receiving MON had greater (P < 0.05) concentrations of palmitic acid (16:0), margaric acid (17:0), docosapentaenoic acid (22:5), and docosahexaenoic acid (22:6) than those yearling bulls receiving PAP. Feeding PAP tended to decrease incidence of rumen lesions and led to similar feedlot performance compared with feeding MON. Thus, PAP is a new technology that presents a possible alternative for ionophores.

[Thyroid pathology in the aged]. / La patologia tiroidea nell'età anziana.

Following a review of the literature on this topic, the authors examine all patients with thyroid pathologies who were admitted to the 1st Institute of Surgical Pathology of the University of Pavia between 1-1-1974 and 31-12-1990. Only patients aged over 65 were included in the study and were subdivided into two groups, namely those aged between 65-74 (3rd age) and those over 75 (4th age). Particular attention was focused on the type of pathology (benign or malignant), other pathologies associated to the basic pathology, the type of operation performed and, in the case of malignant diseases, the histological type of tumour and any metastasis. The results of this series were then compared to those reported elsewhere. The Authors conclude that thyroid pathologies in geriatric patients are very important since, given that at this age the gland is becoming atrophic, the onset of disease often occurs before the age of 65 and symptoms in these patients are imprecise. In view of the good results obtained, it is always worth operating in order to give the patient a reasonable life expectancy, especially in those cases with collateral disorders (compression) caused by the thyroid mass.

[Intestinal occlusions in the elderly patient]. / Occlusioni intestinali nell'anziano.

After reviewing the literature on this topic, the authors examine all patients admitted to the Institute of Surgical Pathology in Pavia with a diagnosis of intestinal occlusion from 1-1-1974 to 31-12-1990. Of these, only those aged over 65 were included in the study and were in turn subdivided into 3rd and 4th age groups. The etiopathogenesis of this disorder is also discussed, stressing that it may be non-neoplastic (Group I) or neoplastic (Group II). The treatment used is also examined (surgical in the majority of cases) together with postoperative complications occurring in these patients. From the results obtained it is concluded that the method used in the pre-, intra- and postoperative stages provided satisfactory results, also in relation to the postoperative mortality rate which relatively low compared to other published reports.

[Acute disorders of the biliary tract in geriatric patients]. / Le affezioni acute delle vie biliari nell'età geriatrica.

Acute pathologies of the biliary tract in geriatric patients were examined in this study taking into account the major causes, treatment used and results obtained. All patients aged over 65 who had been hospitalised during the past 17 years for acute pathologies of the biliary tract (564 cases, equivalent to 34.2% of all in-patients suffering from acute biliary pathologies) were included in the study. These patients were then subdivided into 3rd and 4th age groups (65-74 and < 75 years respectively). The results obtained in the 3rd age group (267 patients, 60%) showed the greatest number of cases of inflammatory lithiasic disease of the cholecystus (61.2%) and VBP (17.7%), whereas 45 patients, equivalent to 49.5%, presented tumours with jaundice. Out of a total of 179 cases in patients in the 4th age group, equivalent to 39.3%, 119 (66.5%) were suffering from lithiasic cholecystitis and 16 (8.9%) from calcolosis of the VBP with jaundice. Cancer of the pancreas head was diagnosed in 27 patients (58.7%), whereas 9 (19.6%) had obstruent cancer of the biliary tract. The Authors conclude that both the preoperative preparation, the choice of operation and postoperative treatment give satisfactory results with a very low early mortality (0.8% in non-tumour cases and 6.9% in tumour cases).

[Hiatal hernia in old age]. / Le ernie iatali nell'età geriatrica.

Following a review of the literature on the topic, the Authors examined all patients admitted to the Institute of Surgical Pathology 1st at the University of Pavia between 1-1-1974 and 31-12-1988 for hiatus hernia, paying special attention to those aged over 65. From an analysis of the results, it was possible to evaluate the influence of collateral pathologies and age-dependent biological changes on the basic disease and the outcome of the operation in elderly patients. In conclusion, this pathology, although not strictly confined to old age, affects a large number of elderly patients, particularly females. It rarely causes acute attacks and it is therefore possible to time the operation to coincide with the patient's best conditions. This is the reason for the excellent results achieved, whereas complications are not related to chronological age but above all to the biological age of the patient.

[A diagnostic protocol for gastric cancer in old age]. / Protocollo diagnostico del cancro gastrico nella III età.

After examining the various diagnostic methods used for gastric cancer, the paper analyses all geriatric patients affected by this disease and hospitalised in the Institute of Pathological Surgery at the University of Pavia between 1/1/1974 and 31/12/1986. The results obtained confirmed that: the subjective symptoms of the elderly patient affected by gastric cancer differ in a large number of cases from those in adults; in some cases, in fact, the disease is silent for long periods of time, whereas it is only slight in others; tests performed revealed neoplasia and possible metastases. In conclusion, although the symptomatology in elderly patients is not clear, some symptoms (weight loss, anemia, etc.) may give the doctor cause for concern. Diagnosis may be made using a variety of tests, ranging from the more commonplace (X-ray of the digestive tract, echography, gastroscopy with biopsy, etc.) to the most recent techniques (immunoscintigraphy).

[Hernias of the abdominal wall in old age]. / Le ernie della parete addominale nell'età anziana.

Following a review of the literature on the topic, the authors examined all patients admitted to the Institute of Surgical Pathology 1st at the University of Pavia between 1-1-1974 and 31-12-1988 affected by hernias of the abdominal wall or surgical laparoceles, paying special attention to those patients aged over 65. Attention was focused on concomitant diseases, the acuteness of the basic pathology and possible recurrence. From the results obtained it was concluded that the associated pathology and the acute state of the basic diseases condition the successful outcome of the operation more than age itself. For this reason, it is important that patients affected by these pathologies are operated as soon as possible in order to carry out an elective operation which reduces postoperative complications and reduces the risk of recurrence.

[Acute abdomen in the elderly]. / L'addome acuto nel vecchio.

After a review of the literature on the subject, all IIIrd and IVth age patients admitted for acute abdomen to the Department of Surgical Pathology I of the University of Pavia between 1-1-1974 and 31-12-1988 were studied. The various pathologies encountered in acute abdomen are considered on the basis of the results obtained, stress being laid on the clinical and diagnostic problems observed in these patients in relation to those of adult age. It is concluded that the study method used in the elderly gave good results with a mortality rate lower than that encountered in other studies on the subject.

[Cancer of the esophagus in the elderly]. / Il cancro dell'esofago nella III età.

After a review of the literature a study was conducted into oesophageal cancers among elderly patients in Pavia University's Institute of Surgical Pathology in 1974-1985. The results led to the following conclusions: this disease occurs most often among the elderly especially those in their sixties; symptoms (dysphagia, weight loss, regurgitation with epigastric and retrosternal pain are initially unclear which makes early diagnosis difficult; most cases require surgery. It is concluded that the appropriate tests should be performed at the first warning signs and that, once the condition is diagnosed, the elderly patient should be prepared for surgery which should be as radical as possible.

[Gastric carcinoma in the aged]. / Il carcinoma gastrico nel vecchio.

After examining the literature on the subject, all patients aged over 60 and admitted to the Surgical Pathology Department of Pavia University from 1-1-1974 to 31-12-1985, suffering from gastric carcinoma have been considered. From the results obtained it is concluded that stomach cancer is a disease that preferentially affects the IIIrd age, usually occurring with atypical symptomatology that leads the patient to underestimate the seriousness of the disease, and impeding early diagnosis that would be effective for therapeutic purposes. It is therefore essential for the necessary examination to be carried out at the first warning symptoms. Further, the general state of these patients has to be controlled and appropriate treatment sought to treat the various accompanying conditions and so prepare the patient for operation.

[Non-neoplastic pathology of the pancreas in the elderly]. / Patologia non neoplastica del pancreas nella terza età.

After examining the literature on the subject, all patients of the IIIrd age suffering from non-neoplastic pathology of the pancreas are considered. The results show that this pathology contains more acute pancreatitis and fewer chronic pancreatitis (at times a consequence of the acute) cases. Pancreatic cyst was also noted. The age most affected by this pathology is the 6th decade and the average age encountered in the series was 60, as is also stated by Camatte and Sarles. It is concluded that the above-mentioned conditions that affect the 6th decade and specially the 60th year of life may be held to involve the IIIrd age.

[Pathology of the bile ducts in the elderly]. / Patologia delle vie biliari nell'età geriatrica.

After examining the literature on the subject, all admissions to the Pavia University Department of Surgical Pathology from 1-1-1974 to 31-12-1985 belonging to the IIIrd age and suffering from non-neoplastic and neoplastic bile way pathology have been reviewed. The two groups were studied on the basis of their histories and laboratory examinations, the outcome of the operation and its complications. It was found that in patients affected by non-neoplastic pathology (lithiasis of the bile ways, alithiasic cholecystitis, etc.) the operation of choice was well tolerated, unlike emergency surgery which in most cases was dangerous. In the IInd group, on the other hand, general condition is very important because on this depends whether or not resective or derivative intervention is carried out. It should be noted here that in all IIIrd age patients associated diseases have to be studied closely for these can contribute to death. It is concluded that in the Ist group of patients, operation can take place in optimal general and local conditions so that the operation can be of choice and not emergency. In the IInd group, as soon as the general condition of the patient so permits, the operation must be as extensive as possible, as age is not an impediment to surgery.

[Peptic ulcer in the elderly]. / L'ulcera peptica nel vecchio.

The Authors, after having reviewed all the literature on this topic, have studied all patients over 60 which were admitted with a diagnosis of peptic ulcer disease at the Institute of Surgical Pathology of the University of Pavia from January 1, 1974 until December 31, 1985. They noted, from their results, that this disease shows a predilection for adults, but is also frequently found in the elderly. In fact, in the latter, during this period of life, the complications which are most frequently observed (especially gastric) are hemorrhage and perforation; according to the Authors the former is due to arteriosclerosis which hinders vascular contraction, while the latter to a reduction of blood flow due to endarteritis, which causes necrosis of the ulcer's base, creating therefore a "locus minoris resistentiae". The Authors conclude that in the elderly, when drug therapy is inefficient, a surgical approach is necessary. In fact, with a well followed pre-operative care program the outcome of surgery is usually satisfactory and complications (mentioned above), which would render surgery and the post-operative period difficult, are prevented.