[Appearance of proliferative activity in peritoneal macrophages cultured in vitro. Mitotic index values and percent frequency of frequency of mitosis phase]. / Sulla comparsa di attività riproduttiva nei macrofagi peritoneali coltivati in vitro. Indici mitotici e frequenza delle fasi mitotiche.

In vitro culture of rat peritoneal macrophages, cells generally considered devoid of proliferative activity, was found to lead to the appearance of mitosis. Mitotic index and percent phase incidence values were determined for these cells.

[Appearance of proliferative activity in peritoneal macrophages cultured in vitro. Stathmokinetic index and proliferative volume]. / L'attività riproduttiva dei macrofagi peritoneali in vitro. Indici statmocinetici e gettito proliferativo.

In the course of further studies of mitotic activity in vitro cultures of rat peritoneal macrophages, an increment in proliferative volume between the 2nd and 6th day was observed, followed by a decrease. The relationships between these variations and those of the mitotic index (previously reported) are discussed.

[Appearance of proliferative activity in peritoneal macrophages cultured in vitro. Karyokinetic chronology and phases]. / Attività mitotica dei macrofagi peritoneali del ratto. Cronologia della cariogenesi e delle sue fasi.

Contrast phase microscopy was employed to determine mitosis phase duration values in rat peritoneal macrophages in which proliferative activity had appeared following culture in vitro. Mitosis duration values were considerable and tended to increase in function of increases in culture time. These data are discussed in a general account of in vitro reproductive activity in peritoneal macrophages.

[Protective effect of cortisone against the anti-cellular action of endotoxins]. / Sull'effetto protettore dei cortisonici nei riguardi della azione anticellulare delle endotossine. Ricerche in vitro mediante il microscopio a contrasto di fase.

In vitro studies with the phase contrast microscope. The protective effect of sodium 6-methyl-prednisolone hemisuccinate against the effects of a purified Salmonella abortus equi lipopolysaccharide were investigated in the rabbit. The intravenous injection of 8 mg of the cortisone derivative simultaneously with the injection of the endotoxin considerably attenuated the febbrile and leukopenic action of the latter. The rate of locomotion of the leukocytes in the peripheral blood in animals treated with endotoxin or with endotoxin plus cortisone was also investigated. In animals protected by sodium 6-methyl-prednisolone hemisuccinate the endotoxin caused a smaller decrease of speed of migration of the leukocytes than that observed in animals treated with the lipopolysaccharide alone. These findings are discussed in the light of present knowledge of the mechanism of the anti-cellular action of endotoxins and of the protective action of cortisone.