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1.
Environ Microbiol Rep ; 12(6): 672-680, 2020 12.
Article in English | MEDLINE | ID: mdl-32975368

ABSTRACT

With rates of community-associated Clostridium difficile infection (CA-CDI) increasing worldwide, potential reservoirs/sources of C. difficile in the community are being sought. Since C. difficile is found in animal manure and human biosolids, which are composted for agricultural purposes, composted products could be a source. In this study, the presence of C. difficile in composted products, and their genetic relatedness to other previously isolated strains from humans, root vegetables and the environment in Western Australia, was investigated. Overall, C. difficile was found in 22.5% (16/71) of composted products [29.7% (11/37) of soil conditioners, 16.7% (2/12) of mulches and 13.6% (3/22) of garden mixes]. Fifteen C. difficile PCR ribotypes (RTs) were identified, the most common toxigenic strains being RTs 020 and 056. Clostridium difficile RT 056 is commonly associated with CDI in humans and has also been isolated from cattle, root vegetables and the environment (veterinary clinics and lawn) in Australia. High-resolution core-genome analysis of 29 C. difficile RT 056 strains revealed clonal relationships between isolates derived from humans, vegetables, composted products and the environment. These findings provide support for an intricate transmission network between human, food and the environment, further highlighting the importance of a 'One Health' approach for managing CDI.


Subject(s)
Clostridioides difficile/classification , Clostridioides difficile/isolation & purification , Clostridium Infections/microbiology , Food Microbiology , Soil Microbiology , Soil/chemistry , Animals , Cattle , Clostridioides difficile/genetics , Feces , Gardens , Humans , Manure/microbiology , Plant Roots/microbiology , Polymerase Chain Reaction , Vegetables/microbiology , Western Australia
2.
Anaerobe ; 54: 55-60, 2018 Dec.
Article in English | MEDLINE | ID: mdl-30053486

ABSTRACT

Antimicrobial resistance (AMR) is commonly found in Clostridium difficile strains and plays a major role in strain evolution. We have previously reported the isolation of large clostridial toxin-negative, binary toxin-producing (A-B-CDT+) C. difficile strains from colonised (and in some instances diarrhoeic) food animals, as well as from patients with diarrhoea. To further characterise these strains, we investigated the phenotypic and genotypic AMR profiles of a diverse collection of A-B-CDT+C. difficile strains. The in vitro activities of 10 antimicrobial agents were determined for 148 A-B-CDT+C. difficile strains using an agar dilution methodology. Whole-genome sequencing and in silico genotyping was performed on 53 isolates to identify AMR genes. All strains were susceptible to vancomycin, metronidazole and fidaxomicin, antimicrobials currently considered first-line treatments for C. difficile infection (CDI). Differences in antimicrobial phenotypes between PCR ribotypes (RTs) were observed but were minimal. Phenotypic resistance was observed in 13 isolates to tetracycline (TetR, MIC = 16 mg/L), moxifloxacin (MxfR, MIC = 16 mg/L), erythromycin (EryR, MIC ≥128 mg/L) and clindamycin (CliR, MIC = 8 mg/L). The MxfR strain (RT033) possessed mutations in gyrA/B, while the TetR (RT033) strain contained a tetM gene carried on the conjugative transposon Tn6190. All EryR and CliR strains (RT033, QX521) were negative for the erythromycin ribosomal methylase gene ermB, suggesting a possible alternative mechanism of resistance. This work describes the presence of multiple AMR genes in A-B-CDT+C. difficile strains and provides the first comprehensive analysis of the AMR repertoire in these lineages isolated from human, animal, food and environmental sources.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Toxins/metabolism , Clostridioides difficile/drug effects , Clostridium Infections/microbiology , Clostridium Infections/veterinary , Drug Resistance, Bacterial , Animals , Cattle , Clindamycin/pharmacology , Clostridioides difficile/genetics , Clostridioides difficile/isolation & purification , Clostridioides difficile/metabolism , Erythromycin/pharmacology , Humans , Metronidazole/pharmacology , Microbial Sensitivity Tests , Ribotyping , Swine , Vancomycin/pharmacology
3.
Int J Antimicrob Agents ; 52(3): 411-415, 2018 Sep.
Article in English | MEDLINE | ID: mdl-29802886

ABSTRACT

We recently reported a high prevalence of Clostridium difficile in retail vegetables, compost and lawn in Western Australia. The objective of this study was to investigate the antimicrobial susceptibility of previously isolated food and environmental C. difficile isolates from Western Australia. A total of 274 C. difficile isolates from vegetables, compost and lawn were tested for susceptibility to a panel of 10 antimicrobial agents (fidaxomicin, vancomycin, metronidazole, rifaximin, clindamycin, erythromycin, amoxicillin/clavulanic acid, moxifloxacin, meropenem and tetracycline) using the agar incorporation method. Fidaxomicin was the most potent agent (MIC50/MIC90, 0.06/0.12 mg/L). Resistance to fidaxomicin and metronidazole was not detected and resistance to vancomycin (0.7%) and moxifloxacin (0.7%) was low. However, 103 isolates (37.6%) showed resistance to at least one agent, and multidrug resistance was observed in 3.9% of the resistant isolates (4/103), all of which came from compost. A significantly greater proportion of compost isolates were resistant to clindamycin, erythromycin and tetracycline compared with food and/or lawn isolates. Clostridium difficile ribotype (RT) 014/020 showed greater clindamycin resistance than other less common RTs (P = 0.008, χ2). Contaminated vegetables, compost and lawn could be playing an intermediary role in the transmission of C. difficile from animals to humans. Environmental strains of C. difficile could also function as a reservoir for antimicrobial resistance genes of clinical relevance. This study provides a baseline for future surveillance of antimicrobial resistance in environmental C. difficile isolates in Australia.


Subject(s)
Anti-Bacterial Agents/pharmacology , Clostridioides difficile/drug effects , Clostridioides difficile/isolation & purification , Drug Resistance, Bacterial , Food Microbiology , Vegetables/microbiology , Australia , Clostridioides difficile/genetics , Composting , Humans , Macrolides/pharmacology , Metronidazole/pharmacology , Microbial Sensitivity Tests , Tetracycline/pharmacology , Vancomycin/pharmacology , beta-Lactams/pharmacology
4.
Anaerobe ; 51: 12-16, 2018 Jun.
Article in English | MEDLINE | ID: mdl-29534914

ABSTRACT

Strains of Clostridium difficile producing only binary toxin (CDT) are found commonly in animals but not humans. However, human diagnostic tests rarely look for CDT. The Cepheid Xpert C. difficile BT assay detects CDT with equal sensitivity (≥92%) in human and animal faecal samples.


Subject(s)
ADP Ribose Transferases/genetics , Bacterial Proteins/genetics , Clostridioides difficile/genetics , Clostridium Infections/diagnosis , Clostridium Infections/veterinary , Molecular Diagnostic Techniques/methods , Animals , Humans , Sensitivity and Specificity
5.
PLoS One ; 12(11): e0187658, 2017.
Article in English | MEDLINE | ID: mdl-29117204

ABSTRACT

Virulence of Clostridium difficile is primarily attributed to the large clostridial toxins A and B while the role of binary toxin (CDT) remains unclear. The prevalence of human strains of C. difficile possessing only CDT genes (A-B-CDT+) is generally low (< 5%), however, this genotype is commonly found in neonatal livestock both in Australia and elsewhere. Zoonotic transmission of C. difficile has been suggested previously. Most human diagnostic tests will not detect A-B-CDT+ strains of C. difficile because they focus on detection of toxin A and/or B. We performed a prospective investigation into the prevalence and genetic characteristics of A-B-CDT+ C. difficile in symptomatic humans. All glutamate dehydrogenase or toxin B gene positive faecal specimens from symptomatic inpatients over 30 days (n = 43) were cultured by enrichment, and C. difficile PCR ribotypes (RTs) and toxin gene profiles determined. From 39 culture-positive specimens, 43 C. difficile isolates were recovered, including two A-B-CDT+ isolates. This corresponded to an A-B-CDT+ prevalence of 2/35 (5.7%) isolates possessing at least one toxin, 2/10 (20%) A-B- isolates, 2/3 CDT+ isolates and 1/28 (3.6%) presumed true CDI cases. No link to Australian livestock-associated C. difficile was found. Neither A-B-CDT+ isolate was the predominant A-B-CDT+ strain found in Australia, RT 033, nor did they belong to toxinotype XI. Previous reports infrequently describe A-B-CDT+ C. difficile in patients and strain collections but the prevalence of human A-B-CDT+ C. difficile is rarely investigated. This study highlights the occurrence of A-B-CDT+ strains of C. difficile in symptomatic patients, warranting further investigations of its role in human infection.


Subject(s)
ADP Ribose Transferases/metabolism , Bacterial Proteins/metabolism , Clostridioides difficile/classification , Clostridioides difficile/genetics , Diarrhea/epidemiology , Diarrhea/microbiology , Ribotyping , Australia/epidemiology , Clostridioides difficile/isolation & purification , Genome, Bacterial , Humans , Polymorphism, Single Nucleotide/genetics , Prevalence
6.
J Clin Microbiol ; 55(1): 216-225, 2017 01.
Article in English | MEDLINE | ID: mdl-27807147

ABSTRACT

Clostridium difficile infection (CDI) is becoming less exclusively a health care-associated CDI (HA-CDI). The incidence of community-associated CDI (CA-CDI) has increased over the past few decades. It has been postulated that asymptomatic toxigenic C. difficile (TCD)-colonized patients may play a role in the transfer of C. difficile between the hospital setting and the community. Thus, to investigate the relatedness of C. difficile across the hospital and community settings, we compared the characteristics of symptomatic and asymptomatic host patients and the pathogens from these patients in these two settings over a 3-year period. Two studies were simultaneously conducted; the first study enrolled symptomatic CDI patients from two tertiary care hospitals and the community in two Australian states, while the second study enrolled asymptomatic TCD-colonized patients from the same tertiary care hospitals. A total of 324 patients (96 with HA-CDI, 152 with CA-CDI, and 76 colonized with TCD) were enrolled. The predominant C. difficile ribotypes isolated in the hospital setting corresponded with those isolated in the community, as it was found that for 79% of the C. difficile isolates from hospitals, an isolate with a matching ribotype was isolated in the community, suggesting that transmission between these two settings is occurring. The toxigenic C. difficile strains causing symptomatic infection were similar to those causing asymptomatic infection, and patients exposed to antimicrobials prior to admission were more likely to develop a symptomatic infection (odds ratio, 2.94; 95% confidence interval, 1.20 to 7.14). Our findings suggest that the development of CDI symptoms in a setting without establishment of hospital epidemics with binary toxin-producing C. difficile strains may be driven mainly by host susceptibility and exposure to antimicrobials, rather than by C. difficile strain characteristics.


Subject(s)
Clostridioides difficile/classification , Clostridium Infections/microbiology , Community-Acquired Infections/microbiology , Cross Infection/microbiology , Ribotyping , Adolescent , Adult , Aged , Aged, 80 and over , Australia/epidemiology , Carrier State/epidemiology , Carrier State/microbiology , Clostridioides difficile/genetics , Clostridioides difficile/isolation & purification , Clostridium Infections/epidemiology , Community-Acquired Infections/epidemiology , Cross Infection/epidemiology , Epidemiologic Studies , Female , Hospitals , Humans , Male , Middle Aged , Molecular Epidemiology , Young Adult
7.
Foodborne Pathog Dis ; 13(12): 647-655, 2016 12.
Article in English | MEDLINE | ID: mdl-27602596

ABSTRACT

Clostridium difficile is the leading cause of antibiotic-associated diarrhea and colitis in hospitalized humans. Recently, C. difficile infection (CDI) has been increasingly recognized as a cause of neonatal enteritis in food animals such as pigs, resulting in stunted growth, delays in weaning, and mortality, as well as colitis in large birds such as ostriches. C. difficile is a strictly anaerobic spore-forming bacterium, which produces two toxins A (TcdA) and B (TcdB) as its main virulence factors. The majority of strains isolated from animals produce an additional binary toxin (C. difficile transferase) that is associated with increased virulence. C. difficile is ubiquitous in the environment and has a wide host range. This review summarizes the epidemiology, clinical presentations, risk factors, and laboratory diagnosis of CDI in animals. Increased awareness by veterinarians and animal owners of the significance of clinical disease caused by C. difficile in livestock and avians is needed. Finally, this review provides an overview on methods for controlling environmental contamination and potential therapeutics available.


Subject(s)
Bird Diseases/microbiology , Clostridioides difficile/pathogenicity , Enterocolitis, Pseudomembranous/veterinary , Animals , Animals, Newborn , Bacterial Proteins/biosynthesis , Bacterial Proteins/isolation & purification , Bacterial Toxins/biosynthesis , Bacterial Toxins/isolation & purification , Bird Diseases/diagnosis , Bird Diseases/physiopathology , Cattle , Cattle Diseases/diagnosis , Cattle Diseases/microbiology , Cattle Diseases/physiopathology , Clostridioides difficile/classification , Clostridioides difficile/isolation & purification , Clostridioides difficile/metabolism , Diarrhea/etiology , Diarrhea/veterinary , Enterocolitis, Pseudomembranous/diagnosis , Enterocolitis, Pseudomembranous/microbiology , Enterocolitis, Pseudomembranous/physiopathology , Enterotoxins/biosynthesis , Enterotoxins/isolation & purification , Feces/microbiology , Livestock , Molecular Typing/veterinary , Struthioniformes , Sus scrofa , Swine , Swine Diseases/diagnosis , Swine Diseases/microbiology , Swine Diseases/physiopathology , Virulence , Virulence Factors/biosynthesis , Virulence Factors/isolation & purification
8.
Anaerobe ; 37: 62-6, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26679487

ABSTRACT

Clostridium difficile is commonly associated with healthcare-related infections in humans, and is an emerging pathogen in food animal species. There is potential for transmission of C. difficile from animals or animal products to humans. This study aimed to determine if C. difficile RT 237 had persisted in a Western Australian piggery or if there had been a temporal change in C. difficile diversity. C. difficile carriage in litters with and without diarrhea was investigated, as was the acquisition of C. difficile over time using cohort surveys. Rectal swabs were obtained from piglets aged 1-10 days to determine prevalence of C. difficile carriage and samples were obtained from 20 piglets on days 1, 7, 13, 20, and 42 of life to determine duration of shedding. Isolation of C. difficile from feces was achieved by selective enrichment culture. All isolates were characterized by standard molecular typing. Antimicrobial susceptibility testing was performed on selected isolates (n = 29). Diarrheic piglets were more likely to shed C. difficile than the non-diseased (p = 0.0124, χ2). In the cohort study, C. difficile was isolated from 40% samples on day 1, 50% on day 7, 20% on day 13, and 0% on days 20 and 42. All isolates were RT 237 and no antimicrobial resistance was detected. The decline of shedding of C. difficile to zero has public health implications because slaughter age pigs have a low likelihood of spreading C. difficile to consumers via pig meat.


Subject(s)
Clostridioides difficile/isolation & purification , Clostridium Infections/veterinary , Swine Diseases/epidemiology , Swine/microbiology , Agriculture , Animals , Australia , Carrier State/veterinary , Clostridium Infections/epidemiology , Clostridium Infections/microbiology , Cohort Studies , Diarrhea/veterinary , Swine Diseases/microbiology
9.
Anaerobe ; 37: 67-71, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26700884

ABSTRACT

Clostridium difficile is an important enteric pathogen of humans and food animals. Recently it has been isolated from retail foods with prevalences up to 42%, prompting concern that contaminated foods may be one of the reasons for increased community-acquired C. difficile infection (CA-CDI). A number of studies have examined the prevalence of C. difficile in raw meats and fresh vegetables; however, fewer studies have examined the prevalence of C. difficile in ready-to-eat meat. The aim of this study was to investigate the in vitro susceptibility of 11 C. difficile isolates of food animal and retail food origins to food preservatives commonly used in ready-to-eat meats. The broth microdilution method was used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) for sodium nitrite, sodium nitrate and sodium metabisulphite against C. difficile. Checkerboard assays were used to investigate the combined effect of sodium nitrite and sodium nitrate, commonly used in combination in meats. Modal MIC values for sodium nitrite, sodium nitrate and sodium metabisulphite were 250 µg/ml, >4000 µg/ml and 1000 µg/ml, respectively. No bactericidal activity was observed for all three food preservatives. The checkerboard assays showed indifferent interaction between sodium nitrite and sodium nitrate. This study demonstrated that C. difficile can survive in the presence of food preservatives at concentrations higher than the current maximum permitted levels allowed in ready-to-eat meats. The possibility of retail ready-to-eat meats contaminated with C. difficile acting as a source of CDI needs to be investigated.


Subject(s)
Clostridioides difficile/drug effects , Food Preservatives/pharmacology , Nitrates/pharmacology , Sodium Nitrite/pharmacology , Sulfites/pharmacology , Animals , Cattle , Food Microbiology , Meat , Microbial Sensitivity Tests , Swine
10.
JMM Case Rep ; 3(4): e005062, 2016 Aug.
Article in English | MEDLINE | ID: mdl-28348781

ABSTRACT

INTRODUCTION: Clostridium difficile infection (CDI) is a significant gastrointestinal disease in the developed world and increasingly recognised as a zoonotic infection. In North America and Europe, the PCR ribotype (RT) 078 strain of C. difficile is commonly found in production animals and as a cause of disease in humans although proof of transmission from animals is lacking. This strain is absent in Australian livestock. We report a case of human CDI caused by a strain of C. difficile belonging to known Australian livestock-associated RT 237. CASE PRESENTATION: A young male was admitted for multiple trauma following a motor vehicle accident and placed on piperacillin/tazobactam for pneumonia. After 4 days of treatment, he developed symptoms of CDI, which was confirmed in the laboratory. His symptoms resolved after 6 days of intravenous metronidazole. The strain of C. difficile isolated was identified as RT 237, an unusual RT previously found in with several Western Australia piggeries. CONCLUSION: This case of CDI caused by an unusual livestock-associated C. difficile RT 237 supports the hypothesis of zoonotic transmission. The case highlights the potential of livestock to act as reservoir for C. difficile and the need for continued surveillance of CDI in both human and animal populations.

11.
BMC Infect Dis ; 15: 516, 2015 Nov 14.
Article in English | MEDLINE | ID: mdl-26573915

ABSTRACT

BACKGROUND: The epidemiology of Clostridium difficile infection (CDI) has changed over the past decades with the emergence of highly virulent strains. The role of asymptomatic C. difficile colonization as part of the clinical spectrum of CDI is complex because many risk factors are common to both disease and asymptomatic states. In this article, we review the role of asymptomatic C. difficile colonization in the progression to symptomatic CDI, describe the epidemiology of asymptomatic C. difficile colonization, assess the effectiveness of screening and intensive infection control practices for patients at risk of asymptomatic C. difficile colonization, and discuss the implications for clinical practice. METHODS: A narrative review was performed in PubMed for articles published from January 1980 to February 2015 using search terms 'Clostridium difficile' and 'colonization' or 'colonisation' or 'carriage'. RESULTS: There is no clear definition for asymptomatic CDI and the terms carriage and colonization are often used interchangeably. The prevalence of asymptomatic C. difficile colonization varies depending on a number of host, pathogen, and environmental factors; current estimates of asymptomatic colonization may be underestimated as stool culture is not practical in a clinical setting. CONCLUSIONS: Asymptomatic C. difficile colonization presents challenging concepts in the overall picture of this disease and its management. Individuals who are colonized by the organism may acquire protection from progression to disease, however they also have the potential to contribute to transmission in healthcare settings.


Subject(s)
Clostridioides difficile/pathogenicity , Clostridium Infections/epidemiology , Clostridium Infections/transmission , Asymptomatic Infections , Bacterial Proteins/metabolism , Bacterial Toxins/metabolism , Clostridium Infections/microbiology , Enterotoxins/metabolism , Host-Pathogen Interactions , Humans , Infection Control , Risk Factors , Spores, Bacterial
12.
J Clin Microbiol ; 53(11): 3702-4, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26354812

ABSTRACT

Large clostridial toxin-negative, binary toxin-positive (A(-) B(-) CDT(+)) strains of Clostridium difficile are almost never associated with clinically significant C. difficile infection (CDI), possibly because such strains are not detected by most diagnostic methods. We report the isolation of an A(-) B(-) CDT(+) ribotype 033 (RT033) strain of C. difficile from a young patient with ulcerative colitis and severe diarrhea.


Subject(s)
ADP Ribose Transferases/genetics , Anti-Bacterial Agents/therapeutic use , Bacterial Proteins/genetics , Bacterial Toxins/genetics , Clostridioides difficile/genetics , Enterocolitis, Pseudomembranous/drug therapy , Enterotoxins/genetics , ADP Ribose Transferases/metabolism , Adolescent , Australia , Bacterial Proteins/metabolism , Clostridioides difficile/isolation & purification , Clostridioides difficile/pathogenicity , Colitis, Ulcerative/microbiology , Enterocolitis, Pseudomembranous/microbiology , Feces/microbiology , Humans , Male , Microbial Sensitivity Tests , Recurrence
13.
J Clin Microbiol ; 53(3): 973-5, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25520452

ABSTRACT

Clostridium difficile PCR ribotype 033 (RT033) is found in the gastrointestinal tracts of production animals and, occasionally, humans. The illumigene C. difficile assay (Meridian Bioscience, Inc.) failed to detect any of 52 C. difficile RT033 isolates, while all strains signaled positive for the binary toxin genes but were reported as negative for C. difficile by the Xpert C. difficile/Epi assay (Cepheid).


Subject(s)
Bacteriological Techniques/methods , Clostridioides difficile/isolation & purification , Clostridium Infections/diagnosis , Clostridium Infections/microbiology , Molecular Diagnostic Techniques/methods , Ribotyping , Animals , Clostridioides difficile/classification , Clostridioides difficile/genetics , Clostridium Infections/veterinary , Humans , Sensitivity and Specificity
14.
Chemistry ; 19(52): 17980-8, 2013 Dec 23.
Article in English | MEDLINE | ID: mdl-24248959

ABSTRACT

Herein we describe the preparation and structure-activity relationship studies on range of stilbene based compounds and their antibacterial activity. Two related compounds, each bearing carboxylic acid moieties, exhibit good activity against several bacterial strains, including methicillin-resistant Staphylococcus aureus MRSA (ATCC 33592 and NCTC 10442). Compound 10 was most active against Moraxella catarrhalis with minimum inhibitory concentrations (MICs) of 0.12-0.25 µg mL(-1) and against Staphylococcus spp. with MICs ranging from 2-4 µg mL(-1). The derivative 17 showed increased activity with MICs of 0.06-0.25 µg mL(-1) against M. catarrhalis and 0.12-1 against Staphylococcus spp. This level of activity is similar to that reported for S. aureus for antibiotics, such as vancomycin, with MICs of ≤2.0 µg mL(-1) and clindamycin with MICs of ≤0.5 µg mL(-1). As an indicator of toxicity, 17 was tested for its ability to lyse sheep erythrocytes, and showed low haemolytic activity. Such results highlight the value of tris(stilbene) compounds as antibacterial agents providing suitable properties for further development.


Subject(s)
Anti-Bacterial Agents/pharmacology , Methicillin-Resistant Staphylococcus aureus/drug effects , Stilbenes/chemistry , Animals , Sheep , Structure-Activity Relationship
15.
Pathology ; 45(5): 495-500, 2013 08.
Article in English | MEDLINE | ID: mdl-23846295

ABSTRACT

AIM: The rapidly changing epidemiology of Clostridium difficile infection highlights the need for improved and continuing surveillance involving stool culturing to enable molecular tracking. Culture of C. difficile can be difficult and time consuming. In this report ChromID C. difficile agar (CDIF) was compared to cycloserine-cefoxitin-fructose-egg-yolk agar which contained 0.1% sodium taurocholate (TCCFA) as a germinant. RESULTS: All ribotypes of C. difficile tested (n=90) grew well on CDIF within 24 h and most gave characteristic small irregular black colonies with a raised umbonate profile. Counts from standard suspensions of C. difficile at 24 h (p<0.005) and 48 h (p=0.01) were significantly higher on CDIF than on TCCFA. Similar results were achieved after alcohol shock. When temperature shock was used to differentiate vegetative cells and spores, the total number of culturable and vegetative cells on CDIF was significantly higher than on TCCFA (culturable cells, p=0.003 at 24 h and p=0.002 at 48 h; vegetative cells, p=0.0003 at 24 h and p=0.0002 at 48 h). CONCLUSIONS: These data suggest that CDIF is a better medium for the recovery of vegetative C. difficile than TCCFA and equal to TCCFA for spore recovery.


Subject(s)
Agar/classification , Cefoxitin , Clostridioides difficile/isolation & purification , Cycloserine , Fructose , Microbiological Techniques/methods , Agar/chemistry , Cefoxitin/analysis , Clostridioides difficile/growth & development , Cycloserine/analysis , Enterocolitis, Pseudomembranous/diagnosis , Enterocolitis, Pseudomembranous/epidemiology , Ethanol , Fructose/analysis , Humans , Ribotyping , Temperature , Time Factors
16.
J Clin Microbiol ; 51(9): 2810-4, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23784128

ABSTRACT

Typing of Clostridium difficile facilitates understanding of the epidemiology of the infection. Some evaluations have shown that certain strain types (for example, ribotype 027) are more virulent than others and are associated with worse clinical outcomes. Although restriction endonuclease analysis (REA) and pulsed-field gel electrophoresis have been widely used in the past, PCR ribotyping is the current method of choice for typing of C. difficile. However, global standardization of ribotyping results is urgently needed. Whole-genome sequencing of C. difficile has the potential to provide even greater epidemiologic information than ribotyping.


Subject(s)
Clostridioides difficile/classification , Clostridioides difficile/genetics , Ribotyping/methods , Ribotyping/standards , Clostridium Infections/epidemiology , Clostridium Infections/microbiology , Humans , Molecular Epidemiology/methods , Molecular Epidemiology/standards , Prohibitins
17.
J Med Microbiol ; 62(Pt 9): 1423-1427, 2013 Sep.
Article in English | MEDLINE | ID: mdl-23579394

ABSTRACT

The culture of toxigenic Clostridium difficile from stool specimens is still seen as the gold standard for the laboratory diagnosis of C. difficile infection (CDI). bioMérieux have released ChromID Cdiff chromogenic agar (CDIF) for the isolation and identification of C. difficile in 24 h. In this study, we compared CDIF to pre-reduced cycloserine-cefoxitin-fructose agar with sodium taurocholate (TCCFA) in the examination of glutamate dehydrogenase-positive faecal specimens that were either GeneOhm positive or negative, using direct culture or culture following alcohol shock. Direct culture on CDIF had a sensitivity of 100 % and recovery of 94 % while for TCCFA these were 87 % and 82 %, respectively. For GeneOhm-positive alcohol-shocked faecal samples, sensitivity and recovery on CDIF was similar to direct culture while on TCCFA they were about 10 % higher. For direct culture, there was a significant difference between growth on CDIF at 24 h and TCCFA at 48 h (P = 0.001) and between the two media at 48 h (P<0.001). A total of 142 strains of C. difficile were recovered in pure culture from all GeneOhm-positive samples used in this study and 11 (7.7 %) of these were A(-)B(-)CDT(-) and may represent mixed infections of toxigenic and non-toxigenic C. difficile. The most dominant ribotype was UK 014 (14.7 %) followed by 002 (11.9 %) and 020 (11.9 %), and 36 % of toxigenic isolates, including an A(-)B(+)CDT(-) strain, could not be assigned a UK ribotype. CDIF outperformed pre-reduced TCCFA by negating the need for alcohol shock treatment and by giving a time saving of 24 h in the isolation of C. difficile. CDIF plates were also more selective than TCCFA and C. difficile colonies were easy to identify and subculture prior to strain typing.


Subject(s)
Agar/chemistry , Bacteriological Techniques/methods , Cefoxitin/chemistry , Clostridioides difficile/isolation & purification , Cycloserine/chemistry , Feces/microbiology , Alcohols/chemistry , Culture Media , Fructose/chemistry , Humans , Reproducibility of Results , Ribotyping , Sensitivity and Specificity , Stress, Physiological , Taurocholic Acid
19.
J Microbiol Methods ; 75(1): 153-5, 2008 Sep.
Article in English | MEDLINE | ID: mdl-18558447

ABSTRACT

VIA medium for Stenotrophomonas maltophilia was modified by substituting meropenem (16 mg/L) for imipenem. S. maltophilia grew from 40% of drains sampled within a hospital and surrounding locations in Perth, Western Australia. The specificity of the new medium for S. maltophilia was 62%, and all contaminating bacteria were easily distinguishable by phenotypic tests and PCR.


Subject(s)
Culture Media/chemistry , Environmental Microbiology , Stenotrophomonas maltophilia/growth & development , Stenotrophomonas maltophilia/isolation & purification , Hospitals
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