ABSTRACT
Interferon epsilon (IFNε) is a unique type I interferon (IFN) that shows distinct constitutive expression in reproductive tract epithelium. Understanding how IFNε expression is regulated is critical for the mechanism of action in protecting the mucosa from infection. Combined computational and experimental investigation of the promoter of IFNε predicted transcription factor binding sites for the ETS family of transcription factors. We demonstrate here that Ifnε is regulated by Elf3, an epithelial restricted member of the ETS family. It is co-expressed with IFNε at the epithelium of uterus, lung and intestine, and we focused on regulation of IFNε expression in the uterus. Promoter reporter studies demonstrated that Elf3 was a strong driver of Ifnε expression; knockdown of Elf3 reduced expression levels of IFNε; Elf3 regulated Ifnε expression and chromatin immunoprecipitation (ChIP) confirmed the direct binding of Elf3 to the IFNε promoter. These data show that Elf3 is important in regulating protective mucosal immunity by driving constitutive expression of IFNε to protect mucosal tissues from infection in at least three organ systems.
ABSTRACT
Conceptualizations of public support for the death penalty that suggest that punitiveness, desire for vengenance, authoritarianism, polital conservatism, or other characteristics generally held in low esteem by many in the academic and research communities are the primary or most significant predictors of citizen responses to this issue are challenged. It is proposed instead that fear of crime, perceptions of increasing crime rates, a belief in the efficacy of punishment as a means of deterrence, and a willingness to employ punishment as a response to criminality have a far more important causal role than has previously been recognized.
