Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility.

Photoinduced antibacterial gold nanoparticles were developed as an alternative for the treatment of antibiotic-resistant bacteria. Thanks to the amoxicillin coating, they possess high in vivo stability, selectivity for the bacteria wall, a good renal clearance, and are completely nontoxic for eukaryotic cells at the bactericidal concentrations. A simple one-step synthesis of amoxi@AuNP is described at mild temperatures using the antibiotic as both reducing and stabilizing agent. Time-resolved fluorescence microscopy proved these novel nano-photosensitizers, with improved selectivity, are bactericidal but showing excellent biocompatibility toward eukaryotic cells at the same dose (1.5 µg/mL) when co-cultures are analyzed. Their stability in biological media, hemocompatibility, and photo-antibacterial effect against sensitive and antibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution were studied in vivo in male Wistar rats. The use of these nanoparticles to treat antibiotic-resistant infections is promising given their high stability and cytocompatibility.

Biocompatibility and photo-induced antibacterial activity of lignin-stabilized noble metal nanoparticles.

One-pot thermal and photochemical syntheses of lignin-doped silver and gold nanoparticles were developed and their antimicrobial properties were studied against Escherichia coli and Staphylococcus aureus. The nature of the lignin as well as the metal are directly involved in the antimicrobial activity observed in these nanocomposites. Whereas one of the nanocomposites is innocuous under dark conditions and shows photoinduced activity only against Staphylococcus aureus, the rest of the lignin-coated silver nanoparticles studied show antimicrobial activity under dark and light conditions for both bacteria strains. Additionally, only photoinduced activity is observed for lignin-coated gold nanoparticles. Importantly, the particles are non-cytotoxic towards human cells at the bactericidal concentrations. Preliminary assays show these silver nanoparticles as potential antimicrobial agents towards S. aureus biofilm eradication.