Looking at the bid picture: A framework for identifying reverse auctions in ecological systems.

Biological market theory can be used to explain intraspecific cooperation, interspecific mutualism, and sexual selection through models of game theory. These models describe the interactions between organisms as two classes of traders (buyers/sellers) exchanging commodities in the form of goods (e.g. food, shelter, matings) and services (e.g. warning calls, protection). Here, we expand biological market theory to include auction theory where bidding serves to match buyers and sellers. In a reverse auction, the seller increases the value of the item or decreases the cost until a buyer steps forward. We provide several examples of ecological systems that may have reverse auctions as underlying mechanisms to form mutualistic relationships. We focus on the yellow baboon (Papio cynocephalus) mating system as a case study to propose how the mechanisms of a reverse auction, which have the unintended but emergent consequence of producing a mutually beneficial outcome that improves collective reproductive benefits of the troop in this multi-female multi-male polygynandrous social system. For the yellow baboon, we posit that the "seller" is the reproductively cycling female, and the "buyer" is a male looking to mate with a cycling female. To the male, the "item for the sale" is the opportunity to sire an offspring, the price is providing safety and foraging time (via consortship) to the female. The "increasing value of the item for sale" is the chance of conception, which increases with each cycle since a female has resumed cycling post-partum. The female's sexual swelling is an honest indicator of that cycle's probability of conception, and since resident males can track a female's cycle since resumption, there is transparency. The males presumably know the chance of conception when choosing to bid by offering consortship. Across nature, this reverse auction game likely exists in other inter- and intraspecific social relationships. Considering an ecological system as a reverse auction broadens our view of social evolution and adaptations through the lens of human economic structures.

A novel field method for preserving African lion (Panthera leo) fecal samples for noninvasive hormone metabolite analysis.

The traditional methods of preserving fecal samples to suspend hormone-degrading bacteria are not always options during remote fieldwork when studying wildlife endocrinology. Our goal was to develop a field method for preserving African lion (Panthera leo) feces for hormone metabolite analysis by determining the stability of fecal hormone metabolites: 1) when exposed to the natural environment, and 2) in silica beads at ambient temperatures. We collected fresh feces from zoo-housed lions and combined them into two (male and female) homogenous samples. Each was divided into eight samples to undergo a cross-designed treatment.•We immediately lyophilized one sample to serve as the control.•We then exposed seven samples outside to natural environmental conditions for 0, 12, 24, 36, 48, 60, or 72 h. After outdoor exposure, we desiccated a subsample in silica beads for an additional 5, 7, or 10 days.•We analyzed the fecal hormone metabolite concentrations in each sample using enzyme immunoassays for corticosterone, cortisol, testosterone, progesterone, and estradiol.We determined that male and female fecal hormone metabolites in fresh African lion fecal samples are stable and comparable to a standardized desiccation method if dried in silica beads for 5 to 10 days prior to storing them at -20℃.