ABSTRACT
BACKGROUND: We presented three cases of enterocolitis due to Yersinia enterocolitica 0:8. METHODS: We use common isolation media, pathogenicity markers and plasmid analysis. RESULTS: The study of genetic and biochemical characteristics of the strains showed that they belonged to 1B biotype, 0:8 serotype, and that they not carry the virulence plasmid. However, they have pathogenicity markers that were considered typical of highly virulent strains. SUMMARY: This are the first isolations of this particular serotype in Spain and they showed, as it happens in other European countries, the spread of serotype 0:8 around the world.
Subject(s)
Diarrhea/microbiology , Yersinia Infections/microbiology , Yersinia enterocolitica/isolation & purification , Acute Disease , Aged , Bacterial Typing Techniques , Female , Humans , Male , Middle Aged , Postoperative Complications/microbiology , Spain/epidemiology , Yersinia Infections/epidemiology , Yersinia enterocolitica/classificationABSTRACT
The evaluation and mechanism of protection against Y. enterocolitica O:8 using different immunization schedules. We immunize 3 groups of 20 mice each against an oral lethal dose of Y. enterocolitica O:8 using: 1) whole cells, 2) hyperimmune sera, and 3) LPS and common antigen. Animals immunized using whole live cells that produce common antigen (O:3 +V and IP614) developed the highest degree of protection, with an overall survival rate of 70% and 60% respectively. Similar results were obtained using animals immunized with sera. Animals immunized using LPS and common antigen also were able to control the replication of the infecting strain. Common antigen and antibodies against it did not inhibit adherence not bacterial invasion. However, it seems to be an important protecting factor, that controls the bacterial replication in intestinal lymph tissue. The exact mechanism is currently unknown.
Subject(s)
Yersinia Infections/prevention & control , Yersinia enterocolitica/immunology , Animals , Antigens, Bacterial/immunology , Female , Immune Sera , Immunization , Immunization, Passive , Lipopolysaccharides/immunology , Mice , Rabbits , Yersinia Infections/immunologyABSTRACT
Two hundred patients that underwent ESWL were randomized into 4 groups in order to determine the benefits of antibiotic prophylaxis. All comparisons among groups were not statistically significant. Neither cephalosporin nor quinolone prophylaxis impacted significantly on bacteriuria rate after ESWL. Thus, in patients without infected stones, urinary tract obstruction and ancillary procedures ESWL could be performed without prophylactic antibiotic regimes.
