The Arbovirus Mapping and Prediction (ArboMAP) system for West Nile virus forecasting.

Objectives: West Nile virus (WNV) is the most common mosquito-borne disease in the United States. Predicting the location and timing of outbreaks would allow targeting of disease prevention and mosquito control activities. Our objective was to develop software (ArboMAP) for routine WNV forecasting using public health surveillance data and meteorological observations. Materials and Methods: ArboMAP was implemented using an R markdown script for data processing, modeling, and report generation. A Google Earth Engine application was developed to summarize and download weather data. Generalized additive models were used to make county-level predictions of WNV cases. Results: ArboMAP minimized the number of manual steps required to make weekly forecasts, generated information that was useful for decision-makers, and has been tested and implemented in multiple public health institutions. Discussion and Conclusion: Routine prediction of mosquito-borne disease risk is feasible and can be implemented by public health departments using ArboMAP.

Comparing Satellite and Ground-Based Measurements of Environmental Suitability for Vector Mosquitoes in an Urban Landscape.

Exposure to mosquito-borne diseases is influenced by landscape patterns and microclimates associated with land cover. These influences can be particularly strong in heterogeneous urban landscapes where human populations are concentrated. We investigated how land cover and climate influenced abundances of Ae. albopictus (Skuse) (Diptera: Culicidae) and Cx. quinquefasciatus (Say) (Diptera: Culicidae) in Norman, Oklahoma (United States). From June-October 2019 and May-October 2020 we sampled mosquitoes along an urban-rural gradient using CO2 baited BG Sentinel traps. Microclimate sensors at these sites measured temperature and humidity. We mapped environmental variables using satellite images from Landsat, Sentinel-2, and VIIRS, and the CHIRPS rainfall dataset. We also obtained meteorological data from the closest weather station. We compared statistical models of mosquito abundance based on microclimate, satellite, weather station, and land cover data. Mosquitoes were more abundant on trap days with higher temperature and relative humidity. Rainfall 2 wk prior to the trap day negatively affected mosquito abundances. Impervious surface cover was positively associated with Cx. quinquefasciatus and tree cover was negatively associated with Ae. albopictus. Among the data sources, models based on satellite variables and land cover data had the best fits for Ae. albopictus (R2 = 0.7) and Cx. quinquefasciatus (R2 = 0.51). Models based on weather station or microclimate data had weaker fits (R2 between 0.09 and 0.17) but were improved by adding land cover variables (R2 between 0.44 and 0.61). These results demonstrate the potential for using satellite remote sensing for mosquito habitat analyses in urban areas.

Genome Sequences of Chikungunya Virus Isolates from Bolivia.

We generated nine coding-complete chikungunya virus genome sequences from blood samples collected during the early 2015 outbreak in Bolivia. Relative to other publicly available chikungunya sequences, the Bolivian samples represent a monophyletic group, suggesting that a single lineage was widely circulating in the country between February and May 2015.