Renal histopathological changes in dogs naturally infected with Ehrlichia canis

Renal involvement in Canine Monocytic Ehrlichiosis (CME) has been demonstrated in chronic cases without histopathological classification of patterns of glomerulopathy. Thus, in this study we proposed to evaluate the histopathological pattern, focusing on the types of glomerulonephritis in kidneys of dogs naturally infected with Ehrlichia canis.Twelve dogs naturally infected with E. canis and six healthy dogs were used. After clinical evaluation and diagnostic confirmation, the animals were euthanized and kidney fragments were obtained for histopathological examination, including hematoxylin and eosin (HE), Massons trichrome, periodic acid-Schiff (PAS), periodic acid-methenamine silver (PAMS) and Congo red stain. Histopathological analysis of dogs with CME demonstrated that major lesions were present in the glomerulus and tubulointerstitial region in 100% of cases. The type of glomerular injury was membranoproliferative in 83.33%, and proliferative in 16.67%. Interstitial nephritis was present in the cortical region of all infected dogs. Minimal to marked lymphohistioplasmacytic inflammatory infiltrate was present in the interstitial, perivascular and periglomerular areas. Differential count of inflammatory cells indicated that lymphocytes predominated in comparison to plasma cells and histiocytes. Interstitial fibrosis and hyaline casts of lower intensity were observed. There was a negative correlation between serum albumin levels and severity of glomerulopathy. In conclusion, membranoproliferative glomerulopathy and interstitial nephritis are the major renal lesions in CME. It has been suggested that the presence of inflammatory infiltrates rich inlymphocytes indicates participation of these infiltrates in the immunopathogenesis of renal lesion in dogs with CME. Furthermore, hypoalbuminemia also seems be a marker of renal damage in dogs infected with E. canis.(AU)

Renal histopathological changes in dogs naturally infected with Ehrlichia canis

Renal involvement in Canine Monocytic Ehrlichiosis (CME) has been demonstrated in chronic cases without histopathological classification of patterns of glomerulopathy. Thus, in this study we proposed to evaluate the histopathological pattern, focusing on the types of glomerulonephritis in kidneys of dogs naturally infected with Ehrlichia canis.Twelve dogs naturally infected with E. canis and six healthy dogs were used. After clinical evaluation and diagnostic confirmation, the animals were euthanized and kidney fragments were obtained for histopathological examination, including hematoxylin and eosin (HE), Massons trichrome, periodic acid-Schiff (PAS), periodic acid-methenamine silver (PAMS) and Congo red stain. Histopathological analysis of dogs with CME demonstrated that major lesions were present in the glomerulus and tubulointerstitial region in 100% of cases. The type of glomerular injury was membranoproliferative in 83.33%, and proliferative in 16.67%. Interstitial nephritis was present in the cortical region of all infected dogs. Minimal to marked lymphohistioplasmacytic inflammatory infiltrate was present in the interstitial, perivascular and periglomerular areas. Differential count of inflammatory cells indicated that lymphocytes predominated in comparison to plasma cells and histiocytes. Interstitial fibrosis and hyaline casts of lower intensity were observed. There was a negative correlation between serum albumin levels and severity of glomerulopathy. In conclusion, membranoproliferative glomerulopathy and interstitial nephritis are the major renal lesions in CME. It has been suggested that the presence of inflammatory infiltrates rich inlymphocytes indicates participation of these infiltrates in the immunopathogenesis of renal lesion in dogs with CME. Furthermore, hypoalbuminemia also seems be a marker of renal damage in dogs infected with E. canis.

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis / Infecção de pardais (Passer domesticus) e diferentes linhagens de camundongos com Lawsonia intracellularis

The susceptibility of sparrows (Passer domesticus) and strains of mice (Swiss, BALB/c, C-57 and DB-A) to Lawsonia intracellularis infection was studied. Thirty-two sparrows were inoculated with pure culture of L. intracellularis and eleven received sham inoculum. Feces were collected on -1, 7, 14 and 21 days post infection (dpi) for detection of L. intracellularis by PCR. After 21 days, all sparrows were euthanized and the tissues processed for histology and immunohistochemistry (IHC). One hundred sixty mice of four different strains (n=40, per strain) were used. For each mouse strain, 16 animals received mucosa homogenate from a pig infected with L. intracellularis, 16 received pure culture of L. intracellularis and eight animals received sham inoculum. Two control and four inoculated mice from each group were euthanized on 7, 14, 21 and 28 dpi. Sections of intestine were collected for histologic analysis and IHC and pooled feces were collected for L. intracellularis PCR. None of the sparrows had any histologic lesions characteristic of proliferative enteropathy or antigen labeling by IHC. All sparrow fecal samples were negative by PCR. All mice strains studied had histopathological lesions typical of PE and IHC labeling consistent with L. intracellularis infection, especially those animals inoculated with pure culture. The most severe lesions were observed in DB-A and Swiss mice. Fecal shedding was detected in all mice strains, with peak at 14 dpi. We conclude that sparrows do not seem to be relevant in the epidemiology of L. intracellularis. The results showed variations in the lesions among the four mice strains used.(AU)

A susceptibilidade de pardais (Passer domesticus) e linhagens de camundongos (Swiss, BALB / C, C-57 e DB-A) à infecção por L. intracellularis foi testada. Trinta e dois pardais foram inoculados com cultura pura de L. intracellularis e onze receberam placebo. As fezes foram coletadas nos dias -1, 7, 14 e 21 após a infecção (dpi) para a detecção de Lawsonia intracellularis por PCR. Após 21 dias, todos os pardais foram eutanasiados e os tecidos processados para a realização da histologia e imuno-histoquímica (IHQ). Cento e sessenta camundongos de quatro linhagens diferentes (n=40, por linhagem) foram utilizados. Para cada linhagem de camundongo, 16 receberam homogeneizado de mucosa preparado a partir de um suíno infectado com L. intracellularis, 16 receberam cultura pura de L. intracellularis e oito animais receberam placebo. Dois camundongos controle e quatro camundongos inoculados de cada grupo foram sacrificados aos 7, 14, 21 e 28 dpi. Seções de intestino foram coletadas para análise histológica e IHQ e amostras de fezes foram coletadas para a realização da PCR para detecção de L. Intracellularis. Nenhum dos pardais apresentou lesões histológicas características da enteropatia proliferativa ou marcação positiva por meio da IHQ. As amostras de fezes dos pardais foram negativas na PCR. Todas as linhagens de camundongos estudadas tinham lesões histopatológicas típicas de enterite proliferativa e IHQ positiva para a infecção por L. intracellularis, especialmente aqueles animais inoculados com a cultura pura. As lesões mais graves foram observadas em camundongos DB-A e Swiss. A eliminação fecal foi detectada em todas as linhagens de camundongos, com pico 14 dpi. Conclui-se que os pardais não são relevantes na disseminação da L. intracellularis. Os resultados mostraram variações nas lesões entre as quatro linhagens de camundongos utilizadas, indicando o potencial risco que os camundongos representam na transmissão de L. Intracellularis.(AU)

Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis / Infecção de pardais (Passer domesticus) e diferentes linhagens de camundongos com Lawsonia intracellularis

The susceptibility of sparrows (Passer domesticus) and strains of mice (Swiss, BALB/c, C-57 and DB-A) to Lawsonia intracellularis infection was studied. Thirty-two sparrows were inoculated with pure culture of L. intracellularis and eleven received sham inoculum. Feces were collected on -1, 7, 14 and 21 days post infection (dpi) for detection of L. intracellularis by PCR. After 21 days, all sparrows were euthanized and the tissues processed for histology and immunohistochemistry (IHC). One hundred sixty mice of four different strains (n=40, per strain) were used. For each mouse strain, 16 animals received mucosa homogenate from a pig infected with L. intracellularis, 16 received pure culture of L. intracellularis and eight animals received sham inoculum. Two control and four inoculated mice from each group were euthanized on 7, 14, 21 and 28 dpi. Sections of intestine were collected for histologic analysis and IHC and pooled feces were collected for L. intracellularis PCR. None of the sparrows had any histologic lesions characteristic of proliferative enteropathy or antigen labeling by IHC. All sparrow fecal samples were negative by PCR. All mice strains studied had histopathological lesions typical of PE and IHC labeling consistent with L. intracellularis infection, especially those animals inoculated with pure culture. The most severe lesions were observed in DB-A and Swiss mice. Fecal shedding was detected in all mice strains, with peak at 14 dpi. We conclude that sparrows do not seem to be relevant in the epidemiology of L. intracellularis. The results showed variations in the lesions among the four mice strains used.

A susceptibilidade de pardais (Passer domesticus) e linhagens de camundongos (Swiss, BALB / C, C-57 e DB-A) à infecção por L. intracellularis foi testada. Trinta e dois pardais foram inoculados com cultura pura de L. intracellularis e onze receberam placebo. As fezes foram coletadas nos dias -1, 7, 14 e 21 após a infecção (dpi) para a detecção de Lawsonia intracellularis por PCR. Após 21 dias, todos os pardais foram eutanasiados e os tecidos processados para a realização da histologia e imuno-histoquímica (IHQ). Cento e sessenta camundongos de quatro linhagens diferentes (n=40, por linhagem) foram utilizados. Para cada linhagem de camundongo, 16 receberam homogeneizado de mucosa preparado a partir de um suíno infectado com L. intracellularis, 16 receberam cultura pura de L. intracellularis e oito animais receberam placebo. Dois camundongos controle e quatro camundongos inoculados de cada grupo foram sacrificados aos 7, 14, 21 e 28 dpi. Seções de intestino foram coletadas para análise histológica e IHQ e amostras de fezes foram coletadas para a realização da PCR para detecção de L. Intracellularis. Nenhum dos pardais apresentou lesões histológicas características da enteropatia proliferativa ou marcação positiva por meio da IHQ. As amostras de fezes dos pardais foram negativas na PCR. Todas as linhagens de camundongos estudadas tinham lesões histopatológicas típicas de enterite proliferativa e IHQ positiva para a infecção por L. intracellularis, especialmente aqueles animais inoculados com a cultura pura. As lesões mais graves foram observadas em camundongos DB-A e Swiss. A eliminação fecal foi detectada em todas as linhagens de camundongos, com pico 14 dpi. Conclui-se que os pardais não são relevantes na disseminação da L. intracellularis. Os resultados mostraram variações nas lesões entre as quatro linhagens de camundongos utilizadas, indicando o potencial risco que os camundongos representam na transmissão de L. Intracellularis.

Clinical and pathological findings of necrotizing meningoencephalitis in a maltese dog

A 2-year-old, intact female Maltese dog was presented to the veterinarian with a history of acute neurological signs. On neurological examination the dog showed deficit of mental status (apathy and depression), seizures, constant howling, head turn and compulsive circling to the right side and falls to the left side. The treatment protocol using prednisolone (for seizures remission) and cyclosporine (initiated in the chronic stage) did not stop the progression of the disease and euthanasia was elected 65 days later. Necropsy revealed mild cerebral asymmetry, and in the frontal ( more affected) parietal and occipital lobes of the right hemisphere there were friable, depressed and yellowish areas characterizing malacia. The left contralateral frontal lobe was edematous and slightly yellowish. At histopathology, the lesions were characterized by marked, multifocal to coalescing necrotizing meningoencephalitis, characterized by focally extensive areas of malacia, especially in the cortex of the frontal and right parietal lobes. Extension of lesions to white matter was observed only in the caudal region of the right frontal lobe. Plasma cells and lymphocytes infiltration was observed around vessels, leptomeninges and in the neuroparenchyma. In addition, the non-cavitation areas were also characterized by neuropil vacuolization, neuronal necrosis, neuronophagia, astroglyosis with...

Clinical and pathological findings of necrotizing meningoencephalitis in a maltese dog

A 2-year-old, intact female Maltese dog was presented to the veterinarian with a history of acute neurological signs. On neurological examination the dog showed deficit of mental status (apathy and depression), seizures, constant howling, head turn and compulsive circling to the right side and falls to the left side. The treatment protocol using prednisolone (for seizures remission) and cyclosporine (initiated in the chronic stage) did not stop the progression of the disease and euthanasia was elected 65 days later. Necropsy revealed mild cerebral asymmetry, and in the frontal ( more affected) parietal and occipital lobes of the right hemisphere there were friable, depressed and yellowish areas characterizing malacia. The left contralateral frontal lobe was edematous and slightly yellowish. At histopathology, the lesions were characterized by marked, multifocal to coalescing necrotizing meningoencephalitis, characterized by focally extensive areas of malacia, especially in the cortex of the frontal and right parietal lobes. Extension of lesions to white matter was observed only in the caudal region of the right frontal lobe. Plasma cells and lymphocytes infiltration was observed around vessels, leptomeninges and in the neuroparenchyma. In addition, the non-cavitation areas were also characterized by neuropil vacuolization, neuronal necrosis, neuronophagia, astroglyosis with...(AU)

Mastitis Accompanied by Lymphadenitis in a Dog Caused by Staphylococcus hyicus

Canine mastitis is an infrequent condition that occurs most commonly in the postpartum period due to ascending bacterial infection. This report aims to describe clinical, pathological and bacteriological aspects of mastitis caused by Staphylococcus hyicus accompanied by disseminated superficial lymphadenitis in a dog. In the postpartum period the animal showed clinical signs of prostration, dyspneia, and unresponsivess to environment. At physical examination, hypothermia, dehydration and subcutaneous edema of the limbs were detected. Gross and histologic features were consistent with marked purulent and necro-hemorrhagic mastitis accompanied by lymphangitis, lymphadenitis and thromboembolic pneumonia. Isolation and biochemical identification confirmed the infection by Staphylococcus hyicus. This study shows that one must be attentive not only of the risk of uterine infection postpartum, but also of the possibility of mammary gland infection.(AU)