An investigation of physiological effects of the Deepwater Horizon oil spill on a long-distance migratory seabird, the northern gannet.

Exposure to oil can have long-term impacts on migratory birds. Following the 2010 Deepwater Horizon blowout in the Gulf of Mexico (GOM), we investigated potential impacts of oil exposure on a population of northern gannets (Morus bassanus) that breed on Bonaventure Island (Québec, Canada) and winter in GOM and along the U.S. Atlantic coast (AC). Blood and feather samples were collected from adults previously equipped with geolocators to determine wintering locations. Parent and alkylated polycyclic aromatic hydrocarbons (PAHs); trace metals; stable isotopes of carbon, nitrogen, and hydrogen; and immune, thyroid, steroid, retinoid, and genetic endpoints were measured. PAH and trace metal concentrations did not differ between gannets using different wintering sites. Feather stable isotope values varied significantly between birds from different wintering locations. Gannets wintering in GOM showed higher feather corticosterone and plasma thyroid hormone levels, which may indicate increased energetic demands and/or greater exposure to environmental stressors.

Potency of polycyclic aromatic hydrocarbons in chicken and Japanese quail embryos.

Birds are receptors of concern for polycyclic aromatic hydrocarbons (PAHs), yet limited data describing the relative potency of PAH congeners are available for avian species. In the present study, we determined embryonic median lethal dose (LD50) values for 5 PAH congeners in chicken (Gallus gallus) and one PAH congener in Japanese quail (Coturnix japonica). Graded concentrations of each test compound were injected into the air cell of chicken or quail eggs before incubation. Embryos were monitored through development (quail) or hatching (chicken). All PAHs tested caused dose-dependent increases in embryo mortality, but few other effects (e.g., weight changes, deformities) were observed. In chicken, windows of developmental sensitivity were identified between embryonic days 4 and 9 and between embryonic days 20 and 22. The rank order potency of benzo[k]fluoranthene (BkF; 76 µg/kg) ≈ dibenz[ah]anthracene (83 µg/kg) > indeno[1,2,3-cd]pyrene (325 µg/kg) > benzo[a]pyrene (461 µg/kg) > benz[a]anthracene (529 µg/kg) corresponded well with previous in vitro estimates in birds. Previously published ethoxyresorufin-O-deethylase median effect concentrations from cultured chicken embryo hepatocytes were highly predictive of our LD50s (p < 0.001, r2 = 0.99). To explore differences in sensitivity between species, Japanese quail eggs were injected with BkF, the most potent PAH. We found that chicken and quail were nearly equally sensitive to BkF. The present results contribute to our developing understanding of variability in responses to PAHs among congeners and species. Environ Toxicol Chem 2018;37:1556-1564. © 2018 SETAC.

Nutritional stress in Northern gannets during an unprecedented low reproductive success year: can extreme sea surface temperature event and dietary change be the cause?

Reproductive success of seabirds is tightly associated with availability of their prey for which the spatiotemporal distribution may be influenced by sea surface temperature (SST) fluctuations. The objective of this study was to investigate whether Northern gannets (Morus bassanus) from the largest colony in North America (Bonaventure Island, Quebec, Canada) were in negative nutritional state during the unprecedented low reproductive success year of 2012, and whether this was associated with changes in SST anomalies and diet. The incubation period of gannets in 2012 was characterized by a significant decline, from early to late incubation, in plasma triglyceride levels that was associated with an increase in plasma corticosterone levels. However, no changes in plasma glycerol and ß-hydroxybutyrate levels were noted. SST anomalies recorded in this area (south of the Gulf of St. Lawrence) during the breeding period were consistently higher in 2012 compared to the previous year (a better reproductive success year). Based on signatures of stable carbon (δ(13)C) and nitrogen (δ(15)N) isotopes in gannet red blood cells and in whole fish homogenates of three major preys (mackerel, herring, and capelin), a minor dietary shift was noted between those years and incubation periods. In light of these findings, it is suggested that the extreme warm-water perturbation event that prevailed in the Gulf of St. Lawrence during summer 2012 was associated with a rapid deterioration of nutritional condition of Bonaventure Island gannets during the incubation. These suboptimal physiological changes likely contributed to the dramatic decline in reproductive success reported in this colony.

Endocrine status of a migratory bird potentially exposed to the Deepwater Horizon oil spill: a case study of northern gannets breeding on Bonaventure Island, Eastern Canada.

The Deepwater Horizon oil spill caused the death of a large number of seabirds in the Gulf of Mexico in 2010. However, the long term consequences of oil exposure on migratory birds overwintering in this area have received limited attention. The present study aimed to investigate the impact of oil contamination (e.g., polycyclic aromatic hydrocarbons (PAHs)) on the circulating status of prolactin and corticosterone, two hormones that influence reproductive success in birds, in Northern gannets (Morus bassanus) breeding on Bonaventure Island, Eastern Canada. Using light-based geolocators, it was found that 23.5% of Northern gannets from Bonaventure Island overwintered in the Gulf of Mexico in 2010-2011; the remainder of this population overwintered along the Atlantic Coast of the United States. PAH concentrations (eight compounds) in gannet blood cells were all found to be under the method limits of quantification, which could be the result of the ability of seabirds to metabolize these compounds and the time elapsed between oil exposure and blood sampling. Corticosterone and prolactin levels as well as body mass did not differ between the two major birds' wintering sites. Moreover, levels of both these hormones did not vary from early to late incubation period. Present results suggest that if Bonaventure Island-breeding Northern gannets had been exposed to oil in the Gulf of Mexico in the aftermath of this historical spill, this exposure could not be associated with changes in hormonal status and body mass in breeding individuals.