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1.
Int J Mol Sci ; 24(13)2023 Jun 28.
Article in English | MEDLINE | ID: mdl-37445969

ABSTRACT

In conventional clinical toxicology practice, the blood level of carboxyhemoglobin is a biomarker of carbon monoxide (CO) poisoning but does not correspond to the complete clinical picture and the severity of the poisoning. Taking into account articles suggesting the relationship between oxidative stress parameters and CO poisoning, it seems reasonable to consider this topic more broadly, including experimental biochemical data (oxidative stress parameters) and patients poisoned with CO. This article aimed to critically assess oxidative-stress-related parameters as potential biomarkers to evaluate the severity of CO poisoning and their possible role in the decision to treat. The critically set parameters were antioxidative, including catalase, 2,2-diphenyl-1-picryl-hydrazyl, glutathione, thiol and carbonyl groups. Our preliminary studies involved patients (n = 82) admitted to the Toxicology Clinical Department of the University Hospital of Jagiellonian University Medical College (Kraków, Poland) during 2015-2020. The poisoning was diagnosed based on medical history, clinical symptoms, and carboxyhemoglobin blood level. Blood samples for carboxyhemoglobin and antioxidative parameters were collected immediately after admission to the emergency department. To evaluate the severity of the poisoning, the Pach scale was applied. The final analysis included a significant decrease in catalase activity and a reduction in glutathione level in all poisoned patients based on the severity of the Pach scale: I°-III° compared to the control group. It follows from the experimental data that the poisoned patients had a significant increase in level due to thiol groups and the 2,2-diphenyl-1-picryl-hydrazyl radical, with no significant differences according to the severity of poisoning. The catalase-to-glutathione and thiol-to-glutathione ratios showed the most important differences between the poisoned patients and the control group, with a significant increase in the poisoned group. The ratios did not differentiate the severity of the poisoning. The carbonyl level was highest in the control group compared to the poisoned group but was not statistically significant. Our critical assessment shows that using oxidative-stress-related parameters to evaluate the severity of CO poisoning, the outcome, and treatment options is challenging.


Subject(s)
Carbon Monoxide Poisoning , Humans , Carbon Monoxide Poisoning/diagnosis , Carbon Monoxide Poisoning/therapy , Catalase , Carboxyhemoglobin/analysis , Carboxyhemoglobin/metabolism , Biomarkers , Oxidative Stress , Antioxidants , Carbon Monoxide , Glutathione
2.
Materials (Basel) ; 15(9)2022 May 05.
Article in English | MEDLINE | ID: mdl-35591648

ABSTRACT

Flowers of red clover (Trifolium pratense), sweet violet (Viola odorata) and elderflowers (Sambucus nigra) were dried by means of air drying at 30 °C and 50 °C and by freeze drying. The content of polyphenols was determined using the Folin-Ciocalteu reagent, while anthocyanins were quantified by the pH differential method. Antioxidant activities of aqueous and ethanolic extracts of the dried flowers were measured by the DPPH and ABTS assays, as well as FRAP and reducing power methods. The highest amount of polyphenols was determined in the ethanolic extracts of fresh red clover flowers (854.76 mg/100 g), while the highest concentration of anthocyanins was determined in the aqueous extracts of fresh sweet violet flowers (99.41 mg/100 g). The results showed that, in general, the extracts of red clover flower were characterized by the highest antioxidant activity, while the sweet violet extracts had the poorest antioxidant properties, although these values fluctuated depending on the method used. There was strong correlation between antioxidant activity and TPC (r = 0.9196, FRAP method). In most cases, freeze drying was found to be the best conservation method, retaining well the antioxidant properties of the tested flowers and the compounds determining these properties.

3.
Materials (Basel) ; 15(7)2022 Apr 02.
Article in English | MEDLINE | ID: mdl-35407947

ABSTRACT

Torrefaction is one of the methods of thermal treatment of biomass, which allows obtaining a product of better quality in the form of biochar. The aim of the paper was to analyze the possibility of using goldenrod (Solidago canadensis, Solidago gigantea) for the production of biochar. The torrefaction process involved the vegetative and generative parts as well as the whole plant at temperatures of 250 °C and 275 °C, for 3 h. Next, the physicochemical properties of the raw material and biochar were determined, namely moisture content, ash content, volatile matter content, calorific value, and heat of combustion. The bulk density of raw biomass and biochar was also determined. It was found that after biomass torrefaction, the ash content, calorific value, and heat of combustion increased, while volatile matter content decreased. It has been observed that in both the case of raw biomass and biochar, the plant species and the sampled parts have a significant impact on the ash content, volatile matter content, calorific value, and heat of combustion.

4.
Materials (Basel) ; 15(5)2022 Mar 06.
Article in English | MEDLINE | ID: mdl-35269182

ABSTRACT

One of the aspects of biological activity of vanadium is its influence on carbohydrate metabolism. For more than 30 years, various vanadium complexes have been tested as antidiabetic agents. This study researched organic vanadium complexes with bipyridinium ligands and their influences on metabolic rate, as well as on the antioxidant activity of adipose tissue. The effects of sodium (2,2'-bipyridine) oxidobisperoxovanadate (V) octahydrate (known as the V complex), bis(2,2'-bipyridine) oxidovanadium (IV) sulfate dehydrate (known as the B complex), and bis(4.4'-dimethyl-2,2'-bipyridine) oxidovanadium (IV) sulfate dihydrate (labelled as the BM complex) were assessed. Solutions of the tested complexes were introduced intraperitoneally with a probe to animals fed with either a control diet or a high-fat diet. The BM complex had a significant influence on the increase in ferric reducing antioxidant power, as well as on the concentration of glutathione in the adipose tissue of rats fed with a high-fat diet. The V complex increased the concentration of glutathione in the adipose tissue of rats fed with control fodder, as well as significantly reduced the relative change in rat weight for the high-fat diet. Furthermore, the presence of each tested vanadium complex had an impact of statistically significant increase in basal metabolic rate, regardless of applied diet. Further research on these organic vanadium complexes is necessary to understand the mechanisms responsible for their ability to affect adipose tissue.

5.
Materials (Basel) ; 15(4)2022 Feb 16.
Article in English | MEDLINE | ID: mdl-35208019

ABSTRACT

So far, there are no results for research on the biomechanical parameters of giant miscanthus stalks taking into account both the influence of moisture content and the internode, from which the samples were taken. Therefore, the aim of the research was to comprehensively investigate the influence of the internode number (NrNod) and water content (MC) on the values of selected biomechanical parameters (modulus of elasticity and maximum stress) determined using various stress tests (three-point bending and compression along the fibers). The research was carried out for dry stalks of different humidities and for different internodes. The results obtained in this study proved that the independent variables of the water content and the internode number cause a statistically significant influence on the values of the examined biomechanical parameters of the miscanthus stem: the modulus of elasticity in compression, the maximum stress in compression, the modulus of elasticity in bending and the maximum stress in bending. The values of the modulus of elasticity (MOE) increase when increasing the NrNod. For individual internodes, MOE values are higher with a higher MC. The values of the maximum stress (σ) also increase when increasing the internode number. For individual internodes, the σ values are lower with a higher MC.

6.
Materials (Basel) ; 13(24)2020 Dec 19.
Article in English | MEDLINE | ID: mdl-33352787

ABSTRACT

The influence of harvest period and drying method of Salvia officinalis L. leaves on the content of essential oils, polyphenols and antioxidant properties was investigated. Sage leaves were collected twice during plant blossoming (in June and July) and dried traditionally in natural conditions and at 35 °C. Antioxidant activity was assayed in methanol-acetone extracts and infusions of dried leaves with the use of free radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP) technique. Total phenolic content in extracts as well as in infusions was determined by the means of Folin-Ciocalteu method. Based on the LC/MS analysis, the polyphenol compounds present in both extracts and in infusions were identified. The extracts contained more polyphenols and were characterized by higher antioxidant activity than infusions. In the extracts significant amount of ferulic acid was found, which was influenced both by the harvest period and drying method. The amount of ferulic acid found in extracts obtained from the June leaves dried traditionally was three times lower (6.185 µg/g DW) than in extracts from July leaves dried in the same conditions. Harvest period had a significant effect on the essential oils' content, leaves collected in July contained 15% more oils than those collected in June.

7.
Sensors (Basel) ; 20(3)2020 Jan 24.
Article in English | MEDLINE | ID: mdl-31991600

ABSTRACT

It is important to correctly predict the microclimate of a greenhouse for control and crop management purposes. Accurately forecasting temperatures in greenhouses has been a focus of research because internal temperature is one of the most important factors influencing crop growth. Artificial Neural Networks (ANNs) are a powerful tool for making forecasts. The purpose of our research was elaboration of a model that would allow to forecast changes in temperatures inside the heated foil tunnel using ANNs. Experimental research has been carried out in a heated foil tunnel situated on the property of the Agricultural University of Krakow. Obtained results have served as data for ANNs. Conducted research confirmed the usefulness of ANNs as tools for making internal temperature forecasts. From all tested networks, the best is the three-layer Perceptron type network with 10 neurons in the hidden layer. This network has 40 inputs and one output (the forecasted internal temperature). As the networks input previous historical internal temperature, external temperature, sun radiation intensity, wind speed and the hour of making a forecast were used. These ANNs had the lowest Root Mean Square Error (RMSE) value for the testing data set (RMSE value = 3.7 °C).

8.
Biomed Res Int ; 2017: 6950516, 2017.
Article in English | MEDLINE | ID: mdl-28529953

ABSTRACT

Vanadium is a trace element mainly connected with regulation of insulin metabolism which is particularly important in diabetes. In recent years, organic complexes of vanadium seem to be more interesting than inorganic salts. Nevertheless, the effect of vanadium on lipid metabolism is still a problematic issue; therefore, the main purpose of this study was to investigate the effect of 3 organic complexes of vanadium such as sodium (2,2'-bipyridine)oxidobisperoxovanadate(V) octahydrate, bis(2,2'-bipyridine)oxidovanadium(IV) sulfate dehydrate, and bis(4,4'-dimethyl-2,2'-bipyridine)oxidovanadium(IV) sulfate dihydrate in conjunction with high-fat as well as control diet in nondiabetes model on the following lipid parameters: total cholesterol, triglycerides, and high density lipoprotein as well as activity of paraoxonase 1. All of these parameters were determined in plasma of Wistar rats. The most significant effect was observed in case of bis(4,4'-dimethyl-2,2' bipyridine)oxidovanadium(IV) sulfate dehydrate in rats fed with high-fat diet. Based on our research, bis(4,4'-dimethyl-2,2'-bipyridine)oxidovanadium(IV) sulfate dihydrate should be the aim of further research and perhaps it will be an important factor in the regulation of lipid metabolism.


Subject(s)
Diabetes Mellitus, Experimental/drug therapy , Hypoglycemic Agents/administration & dosage , Lipid Metabolism/drug effects , Vanadium Compounds/administration & dosage , Animals , Diabetes Mellitus, Experimental/metabolism , Diabetes Mellitus, Experimental/pathology , Diet, High-Fat , Humans , Hypoglycemic Agents/chemistry , Pyridines/administration & dosage , Pyridines/chemistry , Rats , Sulfates/administration & dosage , Sulfates/chemistry , Vanadium/administration & dosage , Vanadium/chemistry , Vanadium Compounds/chemistry
9.
Acta Pol Pharm ; 73(3): 579-88, 2016.
Article in English | MEDLINE | ID: mdl-27476274

ABSTRACT

Clofibric acid derivatives called fibrates, are quite commonly used lipid-lowering drugs, so it is necessary to know beneficial and adverse effects of these compounds on the body. The European Medicines Agency's Committee for Medicinal Products for Human Use (CHMP) has concluded that benefits of four fibrates such as: bezafibrate, ciprofibrate, fenofibrate and gemfibrozil continue outweigh their risk in treatment of people with blood lipid disorders. According to recommendations of the CHMP fibrates should not be used as first-line drugs, except in patients with severe hypertriglyceridemia and patients who cannot use statins. In this paper, we focused on effect of clofibric acid derivatives on lipid metabolism, in particular on apoproteins and regulatory enzymes.


Subject(s)
Clofibric Acid/adverse effects , Clofibric Acid/therapeutic use , Coronary Vessels/drug effects , Coronary Vessels/enzymology , Fibric Acids/adverse effects , Fibric Acids/therapeutic use , Hypolipidemic Agents/adverse effects , Hypolipidemic Agents/therapeutic use , Triglycerides/metabolism , Animals , Humans , Lipid Metabolism/drug effects
10.
ScientificWorldJournal ; 2014: 847368, 2014.
Article in English | MEDLINE | ID: mdl-25401157

ABSTRACT

Cornelian cherry (Cornus mas) is a valuable source of phenolic antioxidants. Flavonoid derivatives as nonenzymatic antioxidants are important in the pathophysiology of many diseases including neurological disorders (e.g., Alzheimer's disease) or heart disease. In this study, we examined the effect of an addition of freeze-dried fruit of cornelian cherry on three types of diets: control diet, fructose diet, and diet enriched in fats (high-fat diet). This effect was studied by determining the following antioxidant parameters in both brain tissue and plasma in rats: catalase, ferric reducing ability of plasma, paraoxonase, protein carbonyl groups, and free thiol groups. Results indicate that both fructose diet and high-fat diet affect the antioxidant capacity of the organism. Furthermore, an addition of cornelian cherry resulted in increased activity of catalase in brain tissue, while in plasma it caused the opposite effect. In turn, with regard to paraoxonase activity in both brain tissue and plasma, it had a stimulating effect. Adding cornelian cherry to the tested diets increased the activity of PON in both tested tissues. Moreover, protective effect of fruits of this plant was observed in the process of oxidation of proteins by decreasing levels of protein carbonyl groups and thiol groups in brain tissue as well as in plasma.


Subject(s)
Antioxidants/administration & dosage , Brain/drug effects , Cornus , Fruit , Neuroprotective Agents/administration & dosage , Plant Extracts/administration & dosage , Animals , Antioxidants/isolation & purification , Brain/metabolism , Flavonoids/administration & dosage , Flavonoids/isolation & purification , Male , Neuroprotective Agents/isolation & purification , Plant Extracts/isolation & purification , Random Allocation , Rats , Rats, Wistar
11.
Biomed Res Int ; 2014: 457085, 2014.
Article in English | MEDLINE | ID: mdl-25057488

ABSTRACT

Aronia juice is considered to be a source of compounds with high antioxidative potential. We conducted a study on the impact of compounds in the Aronia juice on oxidative stress in plasma and brain tissues. The influence of Aronia juice on oxidative stress parameters was tested with the use of a model with a high content of fructose and nonsaturated fats. Therefore, the activity of enzymatic (catalase, CAT, and paraoxonase, PON) and nonenzymatic (thiol groups, SH, and protein carbonyl groups, PCG) oxidative stress markers, which indicate changes in the carbohydrate and protein profiles, was marked in brain tissue homogenates. Adding Aronia caused statistically significant increase in the CAT activity in plasma in all tested diets, while the PON activity showed a statistically significant increase only in case of high fat diet. In animals fed with Aronia juice supplemented with carbohydrates or fat, statistically significant increase in the PON activity and the decrease in the CAT activity in brain tissue were observed. In case of the high fat diet, an increase in the number of SH groups and a decrease in the number of PCG groups in brain tissue were observed.


Subject(s)
Antioxidants/chemistry , Brain/enzymology , Oxidative Stress , Photinia/chemistry , Plant Extracts/chemistry , Animals , Aryldialkylphosphatase/metabolism , Beverages , Brain/drug effects , Catalase/metabolism , Fructose/chemistry , Male , Rats , Rats, Wistar , Sulfhydryl Compounds/chemistry
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