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1.
Reprod Fertil Dev ; 32(5): 531-537, 2020 Mar.
Article in English | MEDLINE | ID: mdl-32087765

ABSTRACT

Ovary fragments from six sexually mature cats were vitrified in the presence or absence of betaine or ascorbic acid, loaded (7.4 or 74µM betaine; 20 or 200µM ascorbic acid) or not (1mM betaine or 0.3mM ascorbic acid) into CaCO3 microparticles, and assessed for follicular morphology, oxidative stress and mitochondrial activity Feline ovarian tissue was successfully preserved after vitrification in the presence of 74µM betaine loaded in CaCO3 microparticles, as confirmed by morphological analysis and the density of preantral follicles and stromal cells, as well as by the increased mitochondrial activity and decreased production of reactive oxygen species.


Subject(s)
Betaine/pharmacology , Calcium Carbonate/pharmacology , Cryopreservation , Mitochondria/drug effects , Ovarian Follicle/drug effects , Reactive Oxygen Species/metabolism , Animals , Ascorbic Acid/pharmacology , Cats , Cell Survival/drug effects , Female , Mitochondria/metabolism , Mitochondria/pathology , Ovarian Follicle/metabolism , Ovarian Follicle/pathology , Vitrification
2.
Photodiagnosis Photodyn Ther ; 25: 111-118, 2019 Mar.
Article in English | MEDLINE | ID: mdl-30468898

ABSTRACT

Rose Bengal@α-cyclodextrin (RB@α-CD) microparticles (µPs) were prepared and the RB inclusion in α-CD was experimentally demonstrated through infrared, UV-VIS absorption spectroscopy and cyclic voltammetry. The RB inclusion in α-CD was theoretically investigated using classical molecular mechanics calculations, the simulation results showing that RB can be included in both the narrow and wide apertures of the α-cyclodextrin ring with configurations exhibiting average binding energies of about 27 kcal mol-1. The prepared RB@α-CD microparticles were characterized through Scanning Electron Microscopy (SEM) and it was demonstrated that they are highly efficient in the photodynamic therapy against a Streptococcus mutans (the main bacteria of cariogenic dental plaque) suspension, as a concentration of RB@α-CD µPs 10 times smaller than the usual concentration of pure RB is still capable to produce significant antibacterial activity.


Subject(s)
Photochemotherapy/methods , Photosensitizing Agents/pharmacology , Rose Bengal/pharmacology , Streptococcus mutans/drug effects , alpha-Cyclodextrins/chemistry , Biofilms , Microscopy, Electron, Scanning , Particle Size , Photosensitizing Agents/administration & dosage , Rose Bengal/administration & dosage , Spectrophotometry, Infrared
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