ABSTRACT
The aim of this study was to evaluate the effects of 7-epiclusianone (7-epi) on specific virulence attributes of Streptococcus mutans in vitro and on development of dental caries in vivo. 7-Epi was obtained and purified from fruits of Rheedia brasiliensis. We investigated its influence on surface-adsorbed glucosyltransferase (Gtf) B activity, acid production, and viability of S. MUTANS in biofilms, as well as on caries development using a rodent model. 7-Epi (100 µg/mL) significantly reduced the activity of surface-adsorbed GtfB (up to 48.0 ± 1.8 of inhibition at 100 µg/mL) and glycolytic pH-drop by S. mutans in biofilms (125 and 250 µg/mL) (vs. vehicle control, p < 0.05). In contrast, the test compound did not significantly affect the bacterial viability when compared to vehicle control (15 % ethanol, p > 0.05). Wistar rats treated topically with 7-epi (twice daily, 60-s exposure) showed significantly smaller number of and less severe smooth- and sulcal-surface carious lesions (p < 0.05), without reducing the S. mutans viable population from the animals' dental biofilms. In conclusion, the natural compound 7-epiclusianone may be a potentially novel pharmacological agent to prevent and control dental caries disease.
Subject(s)
Benzophenones/pharmacology , Benzoquinones/pharmacology , Dental Caries/prevention & control , Streptococcus mutans/drug effects , Animals , Benzophenones/chemistry , Benzophenones/isolation & purification , Benzoquinones/chemistry , Benzoquinones/isolation & purification , Biofilms/drug effects , Clusiaceae/chemistry , Dental Caries/microbiology , Glucans/biosynthesis , Glucosyltransferases/metabolism , Hydrogen-Ion Concentration , Rats , Streptococcus mutans/metabolism , Streptococcus mutans/physiologyABSTRACT
Este estudo avaliou o potencial cariogênico de fórmulas infantis e do leite de vaca, usando um modelo de alto desafio cariogênico em animais. Sessenta ratas Wistar infectadas com Streptococcus sobrinus e dessalivadas foram aleatoriamente divididas em 6 grupos os quais receberam ad libitum:(1) água destilada e esterilizada (ADE) com 5 por cento de sacarose; (2) leite bovino tipo A; (3) Nan 2; (4) Nestogeno 2; (5) Ninho coadjuvante de crescimento; (6) ADE. Os grupos 1 e 6 também receberam dieta essencial NCP#2, através de gavage, 2 vezes/dia. Após 21 dias, os animais foram sacrificados e avaliados quanto a microbiota oral recuperada e o índice de cárie de Keys modificado. A análise dos carboidratos nos leites foi feita através de CLAE. Os resultados foram submetidos aos testes de Shapiro-Wilk e Krusal-Wallis. O leite bovino apresentou o menor potencial cariogênico quando comparado aos outros grupos testes, porém não diferiu estatisticamente do grupo 6. As porcentagens de S. sobrinus dos grupos 1 a 5 não apresentaram diferenças estatisticamente significantes entre si, exceto em relação ao grupo 6. Concluímos que o leite bovino não foi cariogênico e as fórmulas infantis mostraram potencial cariogênico.
Subject(s)
Animals , Female , Rats , Dental Caries/etiology , Cariogenic Agents , Infant Food , Milk , Dietary Carbohydrates/adverse effects , Dietary Carbohydrates/metabolism , Disease Models, Animal , Rats, Wistar , Streptococcus sobrinusABSTRACT
This study evaluated the cariogenic potential of infant formulas and cow's milk, using a high cariogenic challenge in the animal model. Sixty female Wistar rats infected with Streptococcus sobrinus and desalivated were randomly divided into 6 groups, which received ad libitum: 1) sterilized deionized distilled water (SDW) with 5% sucrose; 2) cow's milk; 3) Nan 2; 4) Nestogeno 2; 5) Ninho growth supporting; 6) SDW. Groups 1 and 6 also received essential diet NCP#2 by gavage, twice a day. After 21 days, the animals were killed and evaluated according to recovered oral microbiota and caries score by using a modified Keyes method. The analysis of the carbohydrates in the milk samples was performed using HPLC. The results were analyzed by Shapiro-Wilk and Kruskal-Wallis tests. Cow's milk had the lowest cariogenic potential compared to the other test groups, but it was not statistically different from group 6. The percentage of S. sobrinus obtained from the oral cavity of the animals was not statistically different among the groups studied, except for the SDW group. It was concluded that cow's milk was not cariogenic and infant formulas showed some cariogenic potential.
