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1.
PLoS One ; 19(6): e0296761, 2024.
Article in English | MEDLINE | ID: mdl-38917095

ABSTRACT

Corporate social responsibility (CSR) is a constantly evolving concept that reflects changes in society and the expectations of stakeholders in a process that leads companies to adapt to respond to new challenges and demands. Similarly, the theory and practice of CSR have moved from regulatory compliance to a more holistic approach that requires more sophisticated models that allow for a deeper understanding. Following the fact that companies now have access to a greater amount of information related to their social and environmental performance, as well as comparative data from other organizations, which is why it is necessary to have a management tool that allows collecting, process, and generating more accurate and meaningful metrics that more effectively reflect a company's impact on society. Consequently, the present work aims to identify a system of variables and dimensions representative of the management of organizations that allows the validation of a generic model of corporate social responsibility determined through factorial and structural modeling capable of reflecting more accurate and up-to-date dimensions and metrics of model variables and dimensions. To carry out the study, a sample of 667 middle and senior managers from medium and large companies in Guayas, Ecuador, were randomly contacted in their respective business contexts by trained interviewers. The data processing was carried out in stages. First, the exploratory factorial analysis method of the items was applied to form relevant factors. Then, the dimensions' structural modeling was formulated and ratified through the pertinent goodness-of-fit indices. The results determined a system of correlated factors whose items present estimators of commonality and high and significant factorial loads, excluding variables that did not exceed the sensitization criteria applied. Finally, a model made up of 15 factors and 66 variables ratified using the comparative adjustment indices, the chi-square likelihood ratio, and the mean square error of approximation is introduced, confirming the proposed corporate social responsibility model.


Subject(s)
Social Responsibility , Humans , Models, Theoretical , Commerce
2.
PLoS One ; 19(5): e0303762, 2024.
Article in English | MEDLINE | ID: mdl-38753860

ABSTRACT

The present study, focused on pilgrimages as part of religious tourism, aimed to achieve the following objectives: Identify the motivations of the demand for religious tourism focused on pilgrimages; analyze the segmentation of the demand; identify the relationship between demand segments with satisfaction and loyalty; and establish the sociodemographic aspects that characterize demand segments. The study was conducted during the Pilgrimage of the Christ of Miracles in Lima, Peru. The sample was taken on-site from 384 tourists. The statistical techniques used were factor analysis and the k-means clustering method. The results reveal five motivational dimensions: Religious Experience, Belief Experience, Escape, Touristic Experience, and Shopping. Three attendee segments were also identified: Believers, related to belief experience; Religious, related to religious experience; and Passive, tourists with low motivations. The Religious segment had the highest satisfaction and loyalty levels among these groups. Sociodemographic differences were also found in the demand segments. The findings will contribute to management guidelines for destination administrators with religious events and provide insights into academic literature.


Subject(s)
Motivation , Religion , Tourism , Humans , Peru , Female , Male , Adult , Middle Aged , Young Adult
3.
Heliyon ; 10(5): e26682, 2024 Mar 15.
Article in English | MEDLINE | ID: mdl-38434399

ABSTRACT

This study aimed to identify the motivations of sports tourists to attend Soccer World Cups and to determine the motivational dimensions that predict their satisfaction. The research took place in Doha, the capital of Qatar, during the FIFA World Cup in 2022, where 503 valid questionnaires were collected. Exploratory factor analysis and linear regression with the Enter method were used for data analysis. The results show four motivational dimensions: Sports Passion, Escape & Relaxation, Patriotism & Attachment, and Recreation. Additionally, a positive and significant relationship was found between the Escape & Relaxation factor and overall satisfaction. In contrast, a negative correlation was identified between the Patriotism & Attachment dimension and satisfaction. These findings will contribute to the academic literature related to the Soccer World Cups as part of sports tourism and will also assist sporting event organizers in developing marketing and management plans to benefit this tourist demand.

4.
PLoS One ; 19(3): e0299994, 2024.
Article in English | MEDLINE | ID: mdl-38489322

ABSTRACT

The present work determines the gaps between expectations and perceptions about the quality of the service that patients and families receive in primary health care (PHC) in Guayas, Ecuador. A descriptive, cross-sectional and non-experimental study was carried out, primarily prospective with respect to expectations and retrospective with respect to perceptions of service quality. For its development, a random sample of 533 users from the northern and southern urban sectors of the city of Guayaquil was determined, who were asked to answer a questionnaire. Their responses were collected using a seven-point scale intended to determine magnitudes of gaps, which were confirmed using the Wilcoxon test. The results reveal a significant gap between women, specifically those over 21 years of age who have studied at the technological and university level, and those who work. In general, the five dimensions of quality present significant gaps, highlighting that the lowest gap occurs when the medical professional listens attentively and treats the patient with kindness. Instead, the largest gap occurs because there are no available or easily accessible times for medical appointments. Finally, the dimension that indicates the gaps that service providers best resolve are the empathy items, recording the smallest deviations; On the contrary, the reliability dimension presents the greatest deviation, thus showing higher degrees of dissatisfaction, in both cases significant.


Subject(s)
Patient Satisfaction , Quality of Health Care , Humans , Female , Cross-Sectional Studies , Reproducibility of Results , Ecuador , Prospective Studies , Retrospective Studies , Primary Health Care
5.
J Assist Reprod Genet ; 41(5): 1357-1370, 2024 May.
Article in English | MEDLINE | ID: mdl-38438770

ABSTRACT

PURPOSE: This study aimed to identify a marker for freezability and in vitro fertility of sperm samples before freezing. METHODS: Semen was collected from nine Nelore bulls; half of the ejaculate was used for seminal plasma cell-free DNA (cfDNA) quantification, and the other half was cryopreserved. Evaluation of sperm movement using computer-assisted semen analysis and plasma membrane integrity and stability, acrosomal integrity, apoptosis, and mitochondrial potential using flow cytometry were performed on fresh and frozen/thawed semen at 0, 3, 6, and 12 h after thawing. Frozen/thawed sperm was also used for in vitro embryo production. cfDNA was extracted from each bull, and the total DNA and number of cell-free mitochondrial DNA (cfmtDNA) copies were quantified. Semen from each animal was used for IVF, and cleavage, blastocyst formation, and cell counts were evaluated. RESULTS: Two groups were formed and compared based on the concentrations of cfDNA and cfmDNA present: low-cfDNA and high-cfDNA and low-cfmtDNA and high-cfmtDNA. Up to 12 h post-thawing, there were no differences between the groups in the majority of the sperm parameters evaluated. Cleavage, day 6 and 7 blastocyst rates, and the number of cells were higher in the high cfDNA group than in the low cfDNA group. Similar results were observed for cfmtDNA, except for the number of cells, which was similar between the groups. CONCLUSION: The concentration of cfDNA and the relative number of copies of cfmtDNA in seminal plasma cannot predict the freezability of semen but can be used to predict in vitro embryo production.


Subject(s)
Cell-Free Nucleic Acids , Cryopreservation , Fertilization in Vitro , Semen Analysis , Semen Preservation , Semen , Sperm Motility , Spermatozoa , Animals , Male , Cattle , Cell-Free Nucleic Acids/genetics , Cell-Free Nucleic Acids/blood , Fertilization in Vitro/veterinary , Cryopreservation/veterinary , Semen/metabolism , Semen Analysis/veterinary , Semen Preservation/veterinary , Semen Preservation/methods , Sperm Motility/genetics , Fertility/genetics , Biomarkers , DNA, Mitochondrial/genetics , Blastocyst/metabolism
6.
PLoS One ; 19(3): e0298316, 2024.
Article in English | MEDLINE | ID: mdl-38466703

ABSTRACT

To identify markers of oocyte competence, we compared the biochemical characteristics of fluid and cells from follicles containing oocytes with different capacities to form an embryo. Follicles (5-6 mm) were dissected, and follicular fluid (FF), granulosa cells (GC), cumulus cells (CC) from immature and mature cumulus-oocyte-complexes (COC) were individually collected. The oocytes were matured, fertilized, and cultured individually until day 8 (D8) of development. On D8, the samples were grouped according to embryo production into those that gave rise to blastocysts (EMB) and those that did not reach the blastocyst stage (NEMB). In CCs from immature and mature COCs and GCs, expression of CASP3, SERPINE2, VCAN, LUM, FSHR, EGFR, PGR, and GHR genes was quantified. Cell-free DNA (cfDNA), progesterone, and estradiol concentrations in the FF were determined. Data were analyzed by Mann-Whitney U test (GraphPad Prism 9). GHR was highly expressed in immature CCs from the EMB group, whereas CASP3 was highly expressed in mature CCs from the NEMB group (P<0.05). During maturation, the expression of CASP3 and GHR genes increased only in the NEMB group. ART2 cfDNA was highly detected in FF of the NEMB compared to the EMB group. Progesterone concentration was similar between the groups, whereas estradiol concentration was higher (P<0.05) in the EMB than in the NEMB group. It was concluded that a higher level of GHR transcripts in immature CCs, lower CASP3 expression in CCs from matured COCs, lower levels of ART2, and higher estradiol concentrations in FF may indicate oocytes with greater potential for development.


Subject(s)
Cell-Free Nucleic Acids , Progesterone , Female , Cattle , Animals , Caspase 3/metabolism , Progesterone/metabolism , Serpin E2/metabolism , Oocytes/metabolism , Follicular Fluid/metabolism , Estradiol/metabolism , Cumulus Cells/metabolism , Cell-Free Nucleic Acids/analysis
7.
PLoS One ; 19(1): e0296199, 2024.
Article in English | MEDLINE | ID: mdl-38165885

ABSTRACT

There has been an increase in the interest of tourists in the environment and enjoying it in recent years. This research aims to: a) Identify the motivational dimensions in the protected natural parks and b) Analyze the different segments in the protected natural parks. The research was conducted in the Posets-Maladeta protected area in Spain. The sample comprises 422 surveys got in situ. Data analysis involved the execution of factor analysis and non-hierarchical K-means segmentation. The findings suggest the presence of nine motivational dimensions in the protected natural park studied: self-development, interpersonal relationships, security measures, establishing personal bonds, escape, ego-defensive function, nature, entertainment, and rewards. In addition, within this protected natural park is confirmed the presence of two distinct segments: one focused on nature and the other encompassing multiple motives. The results contribute management guidelines for natural park managers for the benefit of communities and visitors. Additionally, this work can serve as a valuable addition to the academic literature concerning ecotourism.


Subject(s)
Motivation , Recreation , Spain , Surveys and Questionnaires , Leisure Activities , Parks, Recreational
8.
PLoS One ; 19(1): e0294849, 2024.
Article in English | MEDLINE | ID: mdl-38261593

ABSTRACT

The San Pablo community in El Triunfo, Ecuador, is emerging as a promising community-based tourism destination. Despite its potential, the lack of knowledge about self-organization and its implementation has hindered effective tourism management. To address this challenge, a participatory approach was employed, involving the community and key stakeholders, such as the local government of El Triunfo. Through the utilization of Design Thinking and both online and in-person interviews, it was identified that an organizational structure based on networks and a culture of self-organization can drive local tourism. These aspects were incorporated into a Design Thinking-guided process, contributing to the understanding of how to forge an appropriate organizational framework for the community. Furthermore, this study aims for broader impact. The goal is not only to strengthen tourism in San Pablo, but also to inform the management of strategies and policies in other entities. The findings offer valuable insights to similar communities in Ecuador and the region. Collectively, this research enhances the comprehension of community-based tourism and proposes practical solutions for optimizing its management in emerging contexts.


Subject(s)
Knowledge , Tourism , Humans , Ecuador , Local Government , Power, Psychological
9.
Acad Pediatr ; 24(2): 184-189, 2024 Mar.
Article in English | MEDLINE | ID: mdl-37567442

ABSTRACT

OBJECTIVE: Intentionally discussing racism and health equity in clinical reasoning conference may provide an opportunity to reinforce antiracist praxis. We aimed to understand 1) whether these discussions provide a meaningful opportunity to practice applying an antiracist lens in patient care, 2) the feasibility of implementing these discussions in a clinical reasoning format, and 3) the acceptability to Black, Indigenous, and People of Color (BIPOC) and white residents. METHODS: In 2021, 4 clinical reasoning conference pilot sessions were implemented in a pediatrics residency program. Trained faculty facilitated discussions on mitigating inequity in clinical cases. Residents who attended at least 1 session were invited to participate in focus groups, which were analyzed using grounded theory. RESULTS: Thirty residents attended each pilot session out of the 30 to 35 who had the opportunity to attend. The focus groups included 6 BIPOC and 6 white residents. The discussions offered a meaningful opportunity to practice recognizing and naming racism. Having faculty facilitators made the discussions more feasible. Both groups experienced benefits and wanted the discussions to continue, but BIPOC residents want more engagement from their white peers. CONCLUSIONS: Discussing racism and health equity in clinical reasoning conference was a meaningful, feasible, and acceptable opportunity for antiracist praxis.


Subject(s)
Health Equity , Racism , Child , Humans , Problem Solving , Focus Groups , Faculty
10.
PLoS One ; 18(11): e0293480, 2023.
Article in English | MEDLINE | ID: mdl-37917781

ABSTRACT

This research proposes: (i) analyze push and pull motivations in insular marine protected areas, and (ii) determine their effects on tourists' satisfaction, positive recommendations and loyalty in terms of returning. The study was conducted in the Galápagos Islands in Ecuador; a renowned marine protected area with a National Park being a Natural Heritage Site of Humanity by UNESCO. An exploratory (EFA) and confirmatory factor analysis (CFA), followed by a stepwise multiple regression analysis applied on a sample of 407 tourists in the Galápagos Islands showed four push and pull motivational factors in this destination: "passive marine," "active marine," "novelty and escape," and "social relations". "Passive marine" and "social relations" were the most prominent factors influencing tourist satisfaction and return intentions. Moreover, "passive marine" and "novelty and escape " highly influenced visitors' intention to recommend and give positive feedback about this place. These findings constitute action guides for the adequate management of marine protected areas.


Subject(s)
Intention , Motivation , Ecuador , Personal Satisfaction , Regression Analysis
11.
PLoS One ; 18(11): e0293932, 2023.
Article in English | MEDLINE | ID: mdl-37922299

ABSTRACT

The research examines the negative consumer emotions generated by the perception of social networks or traditional media with consumer behavior during the covid_19 pandemic. The study was developed in Peru with a sample of 220 consumers; the design is quantitative and structural equations were used for data processing. The results indicate that social networks and traditional media are not related to negative emotions, but are related to the change in consumer behavior in the purchase of more products and new products. The research has theoretical implications since it provides evidence to the literature that the negative emotions generated during the covid_19 pandemic are related to changes in consumer behavior, which affect the purchase of more products and new products. The practical implications of the research is for businessmen on the causes of changes in consumer behavior generated during crises. like the COVID-19 pandemic.


Subject(s)
COVID-19 , Humans , COVID-19/epidemiology , Emotions , Pandemics , Peru/epidemiology , Consumer Behavior
12.
PLoS One ; 18(9): e0286427, 2023.
Article in English | MEDLINE | ID: mdl-37682931

ABSTRACT

AI-based chatbots are an emerging technology disrupting the tourism industry. Although chatbots have received increasing attention, there is little evidence of their impact on tourists' decisions to visit a destination. This study evaluates the key attributes of chatbots and their effects on user satisfaction and visit intention. We use structural equation modeling with covariance procedures to test the proposed model and its hypotheses. The results showed that informativeness, empathy, and interactivity are critical attributes for satisfaction, which drive tourists' intention to visit a destination.


Subject(s)
Empathy , Intention , Industry , Latent Class Analysis , Artificial Intelligence
13.
PLoS One ; 18(8): e0286923, 2023.
Article in English | MEDLINE | ID: mdl-37527247

ABSTRACT

Coastal tourism offers a wide variety of activities related to nature and culture in a sustainable environment. The present study in a coastal destination with cultural characteristics aims to (i) establish the dimensions of perceived value, (ii) determine the relationship between perceived value and satisfaction, and (iii) identify the relationship between perceived value and loyalty in variables such as return, recommendation, and word of mouth in a cultural coastal destination. This quantitative research used a sample of 384 valid questionnaires collected in Huanchaco, Peru, a city next to the Pacific Ocean, being a coastal destination with cultural potential. Factor analysis and multiple regression were applied for data analysis. The results show three dimensions of value perceived by tourists in coastal destinations: emotional and social value, economic value, and functional value. Of these, emotional and social value is the most salient predictor of tourist satisfaction and loyalty. These results will serve as management guides for cultural coastal destination managers and contribute to the academic literature.


Subject(s)
Personal Satisfaction , Travel , Peru , Tourism , Emotions
14.
Tour Hosp Res ; 23(1): 44-59, 2023 Jan.
Article in English | MEDLINE | ID: mdl-37520686

ABSTRACT

The purpose of this paper was to assess Twitter as a means of communication during tourism crises with the following objectives: (a) identify the topics that are discussed, (b) establish the text sentiment, and (c) determine the differences in gender regarding the topics under discussion and the text sentiment. The data were collected from Twitter between March and April 2020. Using big data software, this study extracted 123,868 tweets globally in different languages through the Twitter API of popular tourism hashtags. Two techniques were applied: word association and sentiment analysis. The results show that the communication made through Tweets has the characteristics of a crisis communication related to the effects of the COVID-19 pandemic in the tourism industry. The theoretical contribution of the research is that Twitter in social media is an effective means of communication during pandemic crises and contributes to reducing negative perceptions and adverse effects of the tourism crises in companies and destinations. The practical contribution of the research is that Twitter can be used as a means of communication helping the communication strategies of companies and organizations.

15.
PLoS One ; 18(6): e0287113, 2023.
Article in English | MEDLINE | ID: mdl-37315091

ABSTRACT

Food festivals have been a growing tourism sector in recent years due to their contributions to a region's economic, marketing, brand, and social growth. This study analyses the demand for the Bahrain food festival. The stated objectives were: i) To identify the motivational dimensions of the demand for the food festival, (ii) To determine the segments of the demand for the food festival, and (iii) To establish the relationship between the demand segments and socio-demographic aspects. The food festival investigated was the Bahrain Food Festival held in Bahrain, located on the east coast of the Persian Gulf. The sample consisted of 380 valid questionnaires and was taken using social networks from those attending the event. The statistical techniques used were factorial analysis and the K-means grouping method. The results show five motivational dimensions: Local food, Art, Entertainment, Socialization, and Escape and novelty. In addition, two segments were found; the first, Entertainment and novelties, is related to attendees who seek to enjoy the festive atmosphere and discover new restaurants. The second is Multiple motives, formed by attendees with several motivations simultaneously. This segment has the highest income and expenses, making it the most important group for developing plans and strategies. The results will contribute to the academic literature and the organizers of food festivals.


Subject(s)
Holidays , Leisure Activities , Bahrain , Atmosphere , Food
16.
PLoS One ; 18(5): e0285138, 2023.
Article in English | MEDLINE | ID: mdl-37200259

ABSTRACT

This study aims to analyze the different segments of urban tourism demand. The data were collected in Mexico City, Lima, Buenos Aires, and Bogota, and a K-means clustering method was used to find the segments. The results showed three segments: the first cluster grouped tourists interested in enjoying lodging and restaurant services; the second included visitors seeking multiple attractions, who were the most willing to recommend the destinations; finally, the third was composed of passive tourists, not drawn to the attractions of these cities. This study contributes to the literature by offering evidence of urban tourism segmentation in Latin American cities, which has been scarcely researched. Furthermore, it sheds light on this topic by finding a segment not previously described in the literature ("multiple attractions"). Finally, this study offers practical implications for managers of tourism companies to plan and improve the competitiveness of destinations based on the different segments found.


Subject(s)
Tourism , Latin America , Cities , Mexico
17.
Mol Reprod Dev ; 90(5): 323-335, 2023 05.
Article in English | MEDLINE | ID: mdl-37039304

ABSTRACT

This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) of pigs cumulus-oocyte complexes (COCs). We evaluated transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD) and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were analyzed in cumulus cells (CCs), increased after 44 h of IVM in the sow group. In the gilt group, only FASN expression increased, while NR3C1 expression decreased after IVM. The measurement of LD in oocytes showed an accumulation of lipids in sow oocytes during IVM, while gilt oocytes showed a decrease in LD. FABP3 and NR3C1 methylation patterns exhibited a demethylation pattern in CCs and oocytes from gilts and sows and showed statistical differences between groups. CCs from sows had a better capacity to change transcription levels of the major genes involved in lipid metabolism during IVM than CCs from gilts. This difference may be involved in accumulation of lipids, acquisition of competence, and maturation of enclosed oocytes. Our results contribute to a better understanding of mechanisms involved in lipid metabolism and acquisition of competence in porcine COCs.


Subject(s)
In Vitro Oocyte Maturation Techniques , Lipid Metabolism , Swine , Animals , Female , In Vitro Oocyte Maturation Techniques/methods , Lipid Metabolism/genetics , Oocytes/metabolism , Sus scrofa , Cumulus Cells/metabolism , Lipids
18.
Methods Mol Biol ; 2647: 37-58, 2023.
Article in English | MEDLINE | ID: mdl-37041328

ABSTRACT

Epigenetics is an area of genetics that studies the heritable modifications in gene expression and phenotype that are not controlled by the primary sequence of DNA. The main epigenetic mechanisms are DNA methylation, post-translational covalent modifications in histone tails, and non-coding RNAs. During mammalian development, there are two global waves of epigenetic reprogramming. The first one occurs during gametogenesis and the second one begins immediately after fertilization. Environmental factors such as exposure to pollutants, unbalanced nutrition, behavioral factors, stress, in vitro culture conditions can negatively affect epigenetic reprogramming events. In this review, we describe the main epigenetic mechanisms found during mammalian preimplantation development (e.g., genomic imprinting, X chromosome inactivation). Moreover, we discuss the detrimental effects of cloning by somatic cell nuclear transfer on the reprogramming of epigenetic patterns and some molecular alternatives to minimize these negative impacts.


Subject(s)
Epigenesis, Genetic , Nuclear Transfer Techniques , Animals , DNA Methylation , Genomic Imprinting , Cloning, Organism , Mammals/genetics
19.
PLoS One ; 18(3): e0281100, 2023.
Article in English | MEDLINE | ID: mdl-36862677

ABSTRACT

This study examines the comments posted on tourism-related YouTube videos during the Covid-19 pandemic to establish sustainable development strategies in destinations. Its objectives were: (i) to identify the topics of discussion, (ii) to establish the perceptions of tourism in a pandemic crisis, and (iii) to identify the destinations mentioned. The data was collected between January and May 2020. 39,225 comments were extracted in different languages and globally through the YouTube API. The data processing was carried out using the word association technique. The results show that the most discussed topics were: "people," "country," "tourist," "place," "tourism," "see," "visit," "travel," "covid-19," "life," and "live," which are the focus of the comments made on the perceptions found and represent the attraction factors shown by the videos and the emotions perceived in the comments. The findings show that users' perceptions are related to risks since the "Covid-19" pandemic is associated with the impact on tourism, people, destinations, and affected countries. The destinations in the comments were: India, Nepal, China, Kerala, France, Thailand, and Europe. The research has theoretical implications concerning tourists' perceptions of destinations since new perceptions associated with destinations during the pandemic are shown. Such concerns involve tourist safety and work at the destinations. This research has practical implications since, during the pandemic, companies can develop prevention plans. Also, governments could implement sustainable development plans that contain measures so that tourists can make their trips during a pandemic.


Subject(s)
COVID-19 , Social Media , Humans , Tourism , COVID-19/epidemiology , Pandemics , Travel
20.
Anim Reprod ; 20(1): e20220076, 2023.
Article in English | MEDLINE | ID: mdl-36938311

ABSTRACT

The establishment of epigenetic marks during the reprogramming window is susceptible to environmental influences, and stimuli during this critical stage can cause altered DNA methylation in offspring. In a previous study, we found that low levels of sulphur and cobalt (low S/Co) in the diet offered to oocyte donors altered the DNA methylome of bovine embryos. However, due to the extensive epigenetic reprogramming that occurs during embryogenesis, we hypothesized that the different methylation regions (DMRs) identified in the blastocysts may not maintain in adulthood. Here, we aimed to characterize DMRs previously identified in embryos, in the blood and sperm of adult progenies of two groups of heifers (low S/Co and control). We used six bulls and characterized the DNA methylation levels of KDM2A, KDM5A, KMT2D, and DOT1L genes. Our results showed that all DMRs analysed in both groups and tissues were hypermethylated unlike that noticed in the embryonic methylome profiles. These results suggest that embryo DMRs were reprogrammed during the final stages of de novo methylation during embryogenesis or later in development. Therefore, due to the highly dynamic epigenetic state during early embryonic development, we suggest that is essential to validate the DMRs found in embryos in adult individuals.

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