ABSTRACT
BACKGROUND: Denture stomatitis is associated to Candida albicans, different bacteria and other co-factors such as an acid pH, a carbohydrate ingestion increase, different systemic illnesses and pharmacological treatments. OBJECTIVE: The aim of this study was to determine Candida albicans, Staphylococcus aureus and Streptococcus mutans prevalence in the mucous membrane and prosthesis of patients with and without atrophic denture stomatitis and its relationship with other potential clinical co-factors. STUDY DESIGN: Saliva was collected from 105 patients (62 female and 43 male) wearing dental prosthesis in order to measure their pH. Oral samples of the mucous membrane and the internal surface of dental prosthesis were taken with sterile cotton to proceed with the microbiological study. The identification of the isolated microorganisms was performed using conventional microbiological methods. RESULTS: Diabetes and Hypertension were the most frequent systemic illnesses. High carbohydrate ingestion was observed in numerous patients. Atrophic denture stomatitis was reported in 50 patients and the pH average in saliva was of 5.2. The presence of C albicans, S. aureus and S. mutans in the mucous membrane and prosthesis was of 51.4%, 52.4% and 67.6%, respectively. C. albicans was isolated in 66.7% from the prosthesis, whereas S. aureus and S. mutans were isolated in 49.5% of those same prosthesis. C. albicans was isolated in 86% of the patients with atrophic denture stomatitis and S. aureus was isolated in a similar percentage (84% of patients). The isolation of S. mutans was less frequent, and it was observed in 16% of the oral samples of these patients. CONCLUSIONS: C. albicans, S. aureus and S. mutans frequently colonize the oral mucous of patients wearing dental prosthesis. This illness-bearing condition is more frequent in patients with denture stomatitis, even though dental prosthesis colonization is lower than in the oral mucous.
Subject(s)
Dental Prosthesis/microbiology , Mouth Mucosa/microbiology , Stomatitis, Denture/microbiology , Aged , Candida albicans/isolation & purification , Dental Prosthesis/adverse effects , Female , Humans , Hydrogen-Ion Concentration , Male , Saliva/microbiology , Staphylococcus aureus/isolation & purification , Streptococcus mutans/isolation & purificationABSTRACT
The aim of the present study was to determine the salivary pH in HIV (Human Immunodeficiency Virus) positive(+) and negative(-) patients and in a control group, for assessing if variations or changes in pH are related to the development of oral candidosis and the species isolated. The sample comprised 120 patients from the Infectology Unit of the Mexico General Hospital, and from the School of Dentistry, UNAM. Three study groups were performed: with oral candidosis HIV+, with oral candidosis HIV-, and a control group. All patients filled out a clinical data questionnaire and signed an informed consent document. A 2 ml sample of non-stimulated saliva was obtained from each patient. The pH was measured and the sample was cultured on dextrose Sabouraud agar. The Candida species determinations were performed by the API 20 C AUX system and statistically analyzed. In the HIV+ group, the pH mean was 6.17, with most prevalence of Candida albicans type I and pseudomembranous candidosis. In the HIV- group prosthesis users, the pH mean was 6.29, with most prevalence of C. albicans type I, but with erythematous candidosis. The control group showed a mean pH of 6.78. A statistically significant difference among pH values was found (F= 15.45 p<0.01). The present study revealed that in HIV+ patients, the most significant predisposing factors are: immunosuppression, antibiotic therapy, bad hygiene, anemia, leucoplakia, and diabetes. The salivary pH with acidic values (more in HIV+ patients) significantly favors candidosis development, specially for C. albicans and C. glabrata species and primarily the pseudomembranous and erythematous clinic types. The pH is not a determinant for Candida growth, but could affect the adherence and invasiveness of the yeast.
ABSTRACT
El objetivo de este reporte fue determinar el efecto del piroxicam (antiinflamatorio no esteroide) sobre las bacterias en el complejo pulpoperiapical, cuando se administra para inhibir el desarrollo de lesiones periapicales experimentales. A 12 ratas cepa long Evans se les provocó exposición pulpar previo a la administración de piroxicam, administrando el medicamento cada 24 horas durante 15 días. Se encontró que el porcentaje de bacterias decrece de la cámara pulpar hacia el periápice observándolos escasos en la lesión periapical en ambos grupos, sin embargo se encuentran incrementados con respecto a porcentajes en el grupo al cual se le administró el piroxicam, lo que sugiere que si bien el piroxicam disminuye el desarrollo de lesiones periapicales, favorece el desarrollo de bacterias incrementando su número en el complejo pulpoperiapical
