ABSTRACT
Rhizobium fredii HH103 produces extracellular signal molecules that are able to induce deformation of root hairs and nodule organogenesis of soybean. This strain produces a large variety of nodulation factors, consisting of a linear backbone of GlcNAc with different degrees of polymerization, bearing on the non-reducing residue various different N-acyl residues. The reducing terminal residue is 2-O-methylfucosylated at position 6. Several analogous molecules substituted with fucose were also detected.
Subject(s)
Lipopolysaccharides/chemistry , Rhizobium/chemistry , Signal Transduction , Chromatography, High Pressure Liquid , Gas Chromatography-Mass Spectrometry , Genistein/pharmacology , Growth Inhibitors/pharmacology , Rhizobium/drug effects , Spectrometry, Mass, Fast Atom BombardmentABSTRACT
The exopolysaccharide produced by a ropy strain of Pediococcus damnosus (2.6) in a semi-defined medium was found to be an homopolymer composed of D-glucose. On the basis of monosaccharide and methylation analysis, 1H, 13C, 1D and 2D NMR experiments the polysaccharide was shown to consist of repeating units with the following structure. [sequence: see text]