Insight into the Mechanism of Lysogeny Control of phiCDKH01 Bacteriophage Infecting Clinical Isolate of Clostridioides difficile.

Clostridioides difficile is a causative agent of antibiotic-associated diarrhea as well as pseudomembranous colitis. So far, all known bacteriophages infecting these bacteria are temperate, which means that instead of prompt lysis of host cells, they can integrate into the host genome or replicate episomally. While C. difficile phages are capable of spontaneous induction and entering the lytic pathway, very little is known about the regulation of their maintenance in the state of lysogeny. In this study, we investigated the properties of a putative major repressor of the recently characterized C. difficile phiCDKH01 bacteriophage. A candidate protein belongs to the XRE family and controls the transcription of genes encoding putative phage antirepressors, known to be involved in the regulation of lytic development. Hence, the putative major phage repressor is likely to be responsible for maintenance of the lysogeny.

The Impact of the First Wave of the COVID-19 Pandemic on University Staff Dietary Behaviours, Sleeping Patterns, and Well-Being: An International Comparison Study.

This study assessed the impact of the first wave of the COVID-19 pandemic on well-being by measuring the changes to food security, dietary behaviour, and sleeping patterns of university staff in England, Poland, Saudi Arabia, and China. Using a cross-sectional study design, participants in four universities in the respective countries were surveyed between June and July 2020. The mean age of the 902 participants was 42 years old and 67% were female. The findings indicate a reduction in emotionally driven food behaviour [t (901.00) = -20.87, p < 0.001], food acquisition location [t (901.00) = -51.55, p < 0.001], skipping meals [t (901.00) = -24, p < 0.001], and consumption of canned fruit and vegetables [t (901.00) = -10.18, p < 0.001]. However, home cooking [t (901.00) = 36.61, p < 0.001] and the food shopping experience [t (901.00) = 4.53, p < 0.001] markedly increased during lockdown. The participants had higher levels of well-being during the pandemic and experienced a significant increase in sleeping hours (p < 0.001). Increased age and sleeping hours were positively associated with overall well-being. Conversely, emotionally driven food behaviour (i.e., buying and eating more food out of boredom/fear or anxiety) and skipping meals decreased the overall well-being. Lockdown had beneficial effects on dietary behaviours, sleeping patterns, and well-being, but there were variations between countries.

"Rear bias" in spatial auditory perception: Attentional and affective vigilance to sounds occurring outside the visual field.

Presented studies explored the rear bias phenomenon, that is, the attentional and affective bias to sounds occurring behind the listener. Physiological and psychological reactions (i.e., fEMG, EDA/SCR, Simple Reaction Task-SRT, and self-assessments of affect-related states) were measured in response to tones of different frequencies (Study 1) and emotional vocalizations (Study 2) presented in rear and front spatial locations. Results showed that emotional vocalizations, when located in the back, facilitate reactions related to attention orientation (i.e., auricularis muscle response and simple reaction times) and evoke higher arousal-both physiological (as measured by SCR) and psychological (self-assessment scale). Importantly, observed asymmetries were larger for negative and threat-related signals (e.g., anger) than positive/nonthreatening ones (e.g., achievement). By contrast, there were only small differences for the relatively higher frequency tones. The observed relationships are discussed in terms of one of the postulated auditory system's functions, which is monitoring of the environment in order to quickly detect potential threats that occur outside of the visual field (e.g., behind one's back).

Longevity, Centenarians and Modified Cellular Proteodynamics.

We have shown before that at least one intracellular proteolytic system seems to be at least as abundant in the peripheral blood lymphocytes of centenarians as in the same cells of young individuals (with the cells of the elderly population showing a significant dip compared to both young and centenarian cohorts). Despite scarce published data, in this review, we tried to answer the question how do different types of cells of longevous people-nonagenarians to (semi)supercentenarians-maintain the quality and quantity of their structural and functional proteins? Specifically, we asked if more robust proteodynamics participate in longevity. We hypothesized that at least some factors controlling the maintenance of cellular proteomes in centenarians will remain at the "young" level (just performing better than in the average elderly). In our quest, we considered multiple aspects of cellular protein maintenance (proteodynamics), including the quality of transcribed DNA, its epigenetic changes, fidelity and quantitative features of transcription of both mRNA and noncoding RNAs, the process of translation, posttranslational modifications leading to maturation and functionalization of nascent proteins, and, finally, multiple facets of the process of elimination of misfolded, aggregated, and otherwise dysfunctional proteins (autophagy). We also included the status of mitochondria, especially production of ATP necessary for protein synthesis and maintenance. We found that with the exception of the latter and of chaperone function, practically all of the considered aspects did show better performance in centenarians than in the average elderly, and most of them approached the levels/activities seen in the cells of young individuals.

Proteolysis dysfunction in the process of aging and age-related diseases.

In this review, we discuss in detail the most relevant proteolytic systems that together with chaperones contribute to creating the proteostasis network that is kept in dynamic balance to maintain overall functionality of cellular proteomes. Data accumulated over decades demonstrate that the effectiveness of elements of the proteostasis network declines with age. In this scenario, failure to degrade misfolded or faulty proteins increases the risk of protein aggregation, chronic inflammation, and the development of age-related diseases. This is especially important in the context of aging-related modification of functions of the immune system.

Moderators of Sexual Interest in Opposite-sex Friends.

The fact that men and women experience sexual attraction toward their opposite-sex friends has been evidenced in various studies. It has also been shown that there is a close parallel between preferences for opposite-sex friends and mate preferences, i.e., that men prioritize physical attractiveness of their OSFs, while women prioritize their male friends' ability to provide protection and economic resources. Although this mating activation hypothesis has been validated to an extent, there is hardly any research that points to moderating factors which would define the boundary conditions for these effects. We present two studies that involved heterosexual participants who were in a committed relationship and at the same time had a heterosexual opposite-sex friend. We investigated how both the qualities of one's current partner and the qualities of one's opposite-sex friend shape sexual interest in opposite-sex friends for men and women. Results mostly support the mating activation hypothesis. We show that within actual cross-sex friendships: 1) physical attractiveness of opposite-sex friends predicts sexual interest toward them, and this effect is stronger for men than women, 2) current partner's attractiveness, provided support, and relationship satisfaction moderate this effect only for women, and not men, 3) perceived financial resources of opposite-sex friends predict sexual interest toward them for highly sexually unrestricted women, and, surprisingly, for those who are in committed relationships with high-income men. The results reaffirm previous evidence indicating that perceptions of opposite-sex friends can be viewed as a manifestation of evolved human mating strategies.

Testing the Disgust-Based Mechanism of Homonegative Attitudes in the Context of the COVID-19 Pandemic.

Negative attitudes and stigmatization can originate from the perception of a disease-related threat. Following the spread of the COVID-19 pandemic, it is often suggested that incidents of discriminatory behavior are the result of defense mechanisms aimed at avoiding pathogens. According to the behavioral immune system theory, people are motivated to distance themselves from individuals who show signs of infection, or who are only heuristically associated with a disease, primarily because of the disgust they evoke. In this paper we focus on negative attitudes toward gay men and lesbians who are among social groups that have been persistently framed as "unclean." In our correlational study (N = 500 heterosexual participants; Polish sample data collected during the first COVID-19 lockdown in Poland, in March/April 2020) we tested moderation models derived from the behavioral immune system theory. Specifically, we investigated whether perceived vulnerability to disease and perceived threat of contracting COVID-19 moderate the relation between disgust and homonegativity. We found that sexual disgust (but not pathogen nor moral disgust) predicted homonegative attitudes. This effect was stronger for participants expressing higher levels of perceived vulnerability to disease but was not dependent on the perception of the COVID-19 threat. The results reaffirm previous evidence indicating a pivotal role of disgust in disease-avoidance mechanisms. They also point to functional flexibility of the behavioral immune system by demonstrating the moderating role of perceived vulnerability to disease in shaping homonegative attitudes. Finally, they show that the threat of COVID-19 does not strengthen the relationship between disgust and homonegativity.

Data from the Human Penguin Project, a cross-national dataset testing social thermoregulation principles.

In the Human Penguin Project (N = 1755), 15 research groups from 12 countries collected body temperature, demographic variables, social network indices, seven widely-used psychological scales and two newly developed questionnaires (the Social Thermoregulation and Risk Avoidance Questionnaire (STRAQ-1) and the Kama Muta Frequency Scale (KAMF)). They were collected to investigate the relationship between environmental factors (e.g., geographical, climate etc.) and human behaviors, which is a long-standing inquiry in the scientific community. More specifically, the present project was designed to test principles surrounding the idea of social thermoregulation, which posits that social networks help people to regulate their core body temperature. The results showed that all scales in the current project have sufficient to good psychometrical properties. Unlike previous crowdsourced projects, this dataset includes not only the cleaned raw data but also all the validation of questionnaires in 9 different languages, thus providing a valuable resource for psychological scientists who are interested in cross-national, environment-human interaction studies.

Power Corrupts, but Control Does Not: What Stands Behind the Effects of Holding High Positions.

People seek high positions not to gain influence over others but to satisfy their need for personal control. Personal control tends to have positive interpersonal consequences. If this is the case, does power indeed corrupt? We argue that holding a high position is associated both with perceptions of power (influence over others) and personal control (influence over one's life). Three studies showed that these two aspects might have opposite consequences: Power over others positively predicted aggressiveness (Study 1, N = 793) and exploitativeness (Study 2, N = 445), whereas personal control predicted these outcomes negatively. In Study 3 ( N = 557), conducted among employees at various organizational positions, the effects of holding a high position on exploitativeness and aggressiveness were differentially mediated by power over others and personal control. We discuss these findings in light of contradicting evidence on the corruptive effects of power.

Registered Replication Report: Dijksterhuis and van Knippenberg (1998).

Dijksterhuis and van Knippenberg (1998) reported that participants primed with a category associated with intelligence ("professor") subsequently performed 13% better on a trivia test than participants primed with a category associated with a lack of intelligence ("soccer hooligans"). In two unpublished replications of this study designed to verify the appropriate testing procedures, Dijksterhuis, van Knippenberg, and Holland observed a smaller difference between conditions (2%-3%) as well as a gender difference: Men showed the effect (9.3% and 7.6%), but women did not (0.3% and -0.3%). The procedure used in those replications served as the basis for this multilab Registered Replication Report. A total of 40 laboratories collected data for this project, and 23 of these laboratories met all inclusion criteria. Here we report the meta-analytic results for those 23 direct replications (total N = 4,493), which tested whether performance on a 30-item general-knowledge trivia task differed between these two priming conditions (results of supplementary analyses of the data from all 40 labs, N = 6,454, are also reported). We observed no overall difference in trivia performance between participants primed with the "professor" category and those primed with the "hooligan" category (0.14%) and no moderation by gender.