[The evaluation of detection rate of Mycoplasma hominis and Ureaplasma spp. using cultural technique and polymerase chain reaction].

In the clinical diagnostic laboratories of Chelyabinsk and St. Petersburg evaluation of detection rate of Mycoplasma hominis and Ureaplasma spp. was implemented using technique of polymerase chain reaction and cultural method. The reagents kits "Mycoplasma ACH-12" and "Ureaplasma ACH-12" were used to detect and determine antibiotics sensitivity of urogenital mycoplasma with determination of character of sensitivity of clinical isolates M.hominis and Ureaplasma spp. in sample of secretion of urethra and cervical channel. The results of study demonstrated that rate of detection of Ureaplasma spp. turned out significantly higher than M.hominis. The common coincidence of results in St. Petersburg between two techniques (polymerase chain reaction and cultural method) in case of detection of Ureaplasma spp. amounted to 97.5% and in case of detection of M.hominis - 93.5%.The common coincidence of the results in Chelyabinsk between two techniques (polymerase chain reaction and cultural method) in case of detection of Ureaplasma spp. amounted to 79.9% and in case of detection of M.hominis - 96.1%.

[The diagnostic significance of content of zinc in seminal fluid of patients with chlamydia affection of urogenital system and decreasing of agility of spermatozoa.]

The study was carried out concerning analysis of content of zinc ions, physical chemical characteristics, kinesiograms and leukocytes of seminal fluid of patients with urogenital chlamydias and healthy males (average age - 35±9.5 years). The detection of zinc ions was implemented using direct colorimetric test without deproteinization. The average content of zinc in patients with chlamydias infection amounted to 1.9 mmol/l. The correlation was established between content of zinc, alteration of mobility, increasing of content of spermatozoa and disorder of motion activity that demonstrates actuality of present study.