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1.
Magn Reson Chem ; 45 Suppl 1: S192-7, 2007 Dec.
Article in English | MEDLINE | ID: mdl-18098352

ABSTRACT

Corticosteroid hormone-induced factor (CHIF) is a major regulatory subunit of the Na,K-ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid-state NMR orientation-dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid-state NMR spectrum measured for (15)N-labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane.


Subject(s)
Cell Membrane , Membrane Proteins/chemistry , Nuclear Magnetic Resonance, Biomolecular/methods , Humans , Lipid Bilayers , Micelles , Protein Conformation
2.
J Bioenerg Biomembr ; 39(5-6): 379-83, 2007 Dec.
Article in English | MEDLINE | ID: mdl-18000745

ABSTRACT

The FXYD membrane proteins constitute a family of conserved auxiliary subunits of the Na,K-ATPase, and have been the focus of recent attention due to their ability to finely regulate the activity of the enzyme complex in various physiological settings. In this review we describe the structures of the proteins, as well as their dynamics and their associations with the lipid bilayer membrane, which we have recently determined by NMR spectroscopy. Although the proteins are relatively small, their genes contain as many as six to nine small exons, and the coincidence of structured protein segments with their genetic elements suggests assembly from discrete structural modules through exon shuffling. The three-dimensional structures and backbone dynamics provide the foundation for understanding their intra-membrane association with the Na,K-ATPase alpha subunit, and the structure of FXYD1 suggests a mechanism whereby the phosphorylation of conserved Ser residues, by protein kinases A and C, could induce a conformational change in the cytoplasmic domain of the protein, to modulate its interaction with the alpha subunit.


Subject(s)
Membrane Proteins/chemistry , Phosphoproteins/chemistry , Sodium-Potassium-Exchanging ATPase/metabolism , Animals , Cell Membrane , Humans , Intracellular Signaling Peptides and Proteins , Lipids , Membrane Proteins/metabolism , Mice , Micelles , Neoplasm Proteins , Phosphoproteins/metabolism , Potassium Channels , Protein Subunits , Rats , Sodium-Potassium-Exchanging ATPase/chemistry
3.
Methods Mol Biol ; 400: 515-29, 2007.
Article in English | MEDLINE | ID: mdl-17951757

ABSTRACT

Nuclear magnetic resonance (NMR) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from gamma and CHIF, two membrane proteins that function as regulatory subunits of the Na+- and K+-ATPase.


Subject(s)
Lipid Bilayers/chemistry , Membrane Proteins/chemistry , Micelles , Nuclear Magnetic Resonance, Biomolecular , Sodium-Potassium-Exchanging ATPase/chemistry , Animals , Gene Expression , Humans , Protein Structure, Secondary , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification , Sodium-Potassium-Exchanging ATPase/biosynthesis , Sodium-Potassium-Exchanging ATPase/isolation & purification
4.
Biochim Biophys Acta ; 1768(12): 3216-24, 2007 Dec.
Article in English | MEDLINE | ID: mdl-17916325

ABSTRACT

The beta-barrels found in the outer membranes of prokaryotic and eukaryotic organisms constitute an important functional class of proteins. Here we present solid-state NMR spectra of the bacterial outer membrane protein OmpX in oriented lipid bilayer membranes. We show that OmpX is folded in both glass-supported oriented lipid bilayers and in lipid bicelles that can be magnetically oriented with the membrane plane parallel or perpendicular to the direction of the magnetic field. The presence of resolved peaks in these spectra demonstrates that OmpX undergoes rotational diffusion around an axis perpendicular to the membrane surface. A tightly hydrogen-bonded domain of OmpX resists exchange with D2O for days and is assigned to the transmembrane beta-barrel, while peaks at isotropic resonance frequencies that disappear rapidly in D2O are assigned to the extracellular and periplasmic loops. The two-dimensional 1H/15N separated local field spectra of OmpX have several resolved peaks, and agree well with the spectra calculated from the crystal structure of OmpX rotated with the barrel axis nearly parallel (5 degrees tilt) to the direction of the magnetic field. The data indicate that it will be possible to obtain site-specific resonance assignments and to determine the structure, tilt, and rotation of OmpX in membranes using the solid-state NMR methods that are currently being applied to alpha-helical membrane proteins.


Subject(s)
Bacterial Outer Membrane Proteins/chemistry , Escherichia coli Proteins/chemistry , Hydrolases/chemistry , Lipid Bilayers/chemistry , Magnetic Resonance Spectroscopy/methods , Amino Acid Sequence , Bacterial Outer Membrane Proteins/metabolism , Electrophoresis, Polyacrylamide Gel , Escherichia coli Proteins/metabolism , Hydrolases/metabolism , Molecular Sequence Data
6.
Biochemistry ; 46(23): 6774-83, 2007 Jun 12.
Article in English | MEDLINE | ID: mdl-17511473

ABSTRACT

FXYD1 is a major regulatory subunit of the Na,K-ATPase and the principal substrate of hormone-regulated phosphorylation by c-AMP dependent protein kinases A and C in heart and skeletal muscle sarcolemma. It is a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here, we present the three-dimensional structure of FXYD1 determined in micelles by NMR spectroscopy. Structure determination was made possible by measuring residual dipolar couplings in weakly oriented micelle samples of the protein. This allowed us to obtain the relative orientations of the helical segments and information about the protein dynamics. The structural analysis was further facilitated by the inclusion of distance restraints, obtained from paramagnetic spin label relaxation enhancements, and by refinement with a micelle depth restraint, derived from paramagnetic Mn line broadening effects. The structure of FXYD1 provides the foundation for understanding its intra-membrane association with the Na,K-ATPase alpha subunit and suggests a mechanism whereby the phosphorylation of conserved Ser residues, by protein kinases A and C, could induce a conformational change in the cytoplasmic domain of the protein to modulate its interaction with the alpha subunit.


Subject(s)
Membrane Proteins/chemistry , Phosphoproteins/chemistry , Sodium-Potassium-Exchanging ATPase/metabolism , Animals , Cell Line, Tumor , Electron Spin Resonance Spectroscopy , Female , Freeze Drying , Humans , Magnetic Resonance Spectroscopy , Membrane Proteins/isolation & purification , Membrane Proteins/metabolism , Micelles , Phosphoproteins/isolation & purification , Phosphoproteins/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Xenopus
7.
Methods ; 41(4): 398-408, 2007 Apr.
Article in English | MEDLINE | ID: mdl-17367712

ABSTRACT

Determining the atomic resolution structures of membrane proteins is of particular interest in contemporary structural biology. Helical membrane proteins constitute one-third of the expressed proteins encoded in a genome, many drugs have membrane-bound proteins as their receptors, and mutations in membrane proteins result in human diseases. Although integral membrane proteins provide daunting technical challenges for all methods of protein structure determination, nuclear magnetic resonance (NMR) spectroscopy can be an extremely versatile and powerful method for determining their structures and characterizing their dynamics, in lipid environments that closely mimic the cell membranes. Once milligram amounts of isotopically labeled protein are expressed and purified, micelle samples can be prepared for solution NMR analysis, and lipid bilayer samples can be prepared for solid-state NMR analysis. The two approaches are complementary and can provide detailed structural and dynamic information. This paper describes the steps for membrane protein structure determination using solution and solid-state NMR. The methods for protein expression and purification, sample preparation and NMR experiments are described and illustrated with examples from the FXYD proteins, a family of regulatory subunits of the Na,K-ATPase.


Subject(s)
Lipid Bilayers/chemistry , Membrane Proteins/chemistry , Micelles , Nuclear Magnetic Resonance, Biomolecular/methods , Phosphoproteins/chemistry , Amino Acid Sequence , Cell Line, Transformed , Detergents , Gene Expression , Intracellular Signaling Peptides and Proteins/chemistry , Intracellular Signaling Peptides and Proteins/genetics , Membrane Proteins/genetics , Molecular Sequence Data , Neoplasm Proteins/chemistry , Neoplasm Proteins/genetics , Nitrogen Isotopes , Phosphoproteins/genetics , Phosphorus Isotopes , Plasmids , Potassium Channels/chemistry , Potassium Channels/genetics , Protein Structure, Tertiary , Sodium-Potassium-Exchanging ATPase/chemistry , Sodium-Potassium-Exchanging ATPase/genetics
8.
J Mol Biol ; 356(4): 876-85, 2006 Mar 03.
Article in English | MEDLINE | ID: mdl-16403516

ABSTRACT

Anginex is a synthetic beta-sheet peptide with anti-angiogenic and anti-tumor activity. When added to cultured endothelial cells at concentrations ranging from 2.5 microM to 25 microM, anginex induced cell death, which was reflected by a strong increase of subdiploid cells and fragments, loss of cellular ATP, and LDH release. Cytotoxicity remained the same whether cells were treated with anginex at 4 degrees C or at 37 degrees C. At low temperatures, fluorescein-conjugated anginex accumulated on the endothelial surface, but did not reach into the cytoplasm, indicating that the cell membrane is the primary target for the peptide. Within minutes of treatment, anginex caused endothelial cells to take up propidium iodide and undergo depolarization, both parameters characteristic for permeabilization of the cell membrane. This process was amplified when cells were activated with hydrogen peroxide. Red blood cell membranes were essentially unaffected by anginex. Anginex bound lipid bilayers with high affinity and with a clear preference for anionic over zwitterionic phospholipids. Structural studies by circular dichroism and solid-state nuclear magnetic resonance showed that anginex forms a beta-sheet and adopts a unique and highly ordered conformation upon binding to lipid membranes. This is consistent with lipid micellization or the formation of pore-forming beta-barrels. The data suggest that the cytotoxicity of anginex stems from its ability to target and disrupt the endothelial cell membrane, providing a possible explanation for the angiostatic activity of the peptide.


Subject(s)
Cell Membrane/drug effects , Endothelial Cells/drug effects , Proteins/metabolism , Proteins/pharmacology , Cell Death , Cell Membrane/metabolism , Cell Membrane Permeability/drug effects , Cells, Cultured , Endothelial Cells/cytology , Endothelial Cells/metabolism , Endothelium, Vascular/cytology , Humans , Lipid Bilayers/chemistry , Liposomes , Peptides , Protein Conformation , Proteins/chemistry
9.
J Mol Biol ; 354(4): 743-50, 2005 Dec 09.
Article in English | MEDLINE | ID: mdl-16288923

ABSTRACT

The FXYD family proteins are auxiliary subunits of the Na,K-ATPase, expressed primarily in tissues that specialize in fluid or solute transport, or that are electrically excitable. These proteins range in size from about 60 to 160 amino acid residues, and share a core homology of 35 amino acid residues in and around a single transmembrane segment. Despite their relatively small sizes, they are all encoded by genes with six to nine small exons. We show that the helical secondary structures of three FXYD family members, FXYD1, FXYD3, and FXYD4, determined in micelles by NMR spectroscopy, reflect the structures of their corresponding genes. The coincidence of helical regions, and connecting segments, with the positions of intron-exon junctions in the genes, support the hypothesis that the FXYD proteins may have been assembled from discrete structural modules through exon shuffling.


Subject(s)
Sodium-Potassium-Exchanging ATPase/chemistry , Sodium-Potassium-Exchanging ATPase/genetics , Animals , Exons , Gene Components , Gene Rearrangement , Humans , Intracellular Signaling Peptides and Proteins/chemistry , Intracellular Signaling Peptides and Proteins/genetics , Membrane Proteins/chemistry , Membrane Proteins/genetics , Mice , Micelles , Neoplasm Proteins/chemistry , Neoplasm Proteins/genetics , Nuclear Magnetic Resonance, Biomolecular , Phosphoproteins/chemistry , Phosphoproteins/genetics , Potassium Channels/chemistry , Potassium Channels/genetics , Protein Structure, Secondary , Rats , Sodium Dodecyl Sulfate
10.
Protein Sci ; 14(4): 948-55, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15741345

ABSTRACT

An Escherichia coli plasmid vector for the high-level expression of hydrophobic membrane proteins is described. The plasmid, pBCL, directs the expression of a target polypeptide fused to the C terminus of a mutant form of the anti-apoptotic Bcl-2 family protein, Bcl-XL, where the hydrophobic C terminus has been deleted, and Met residues have been mutated to Leu to facilitate CNBr cleavage after a single Met inserted at the beginning of the target sequence. Fusion protein expression is in inclusion bodies, simplifying the protein purification steps. Here we report the high-level production of PLM, a membrane protein that is a member of the FXYD family of tissue-specific and physiological-state-specific auxiliary subunits of the Na,K-ATPase, expressed abundantly in heart and skeletal muscle. We demonstrate that milligram quantities of pure, isotopically labeled protein can be obtained easily and in little time with this system.


Subject(s)
Membrane Proteins/genetics , Proto-Oncogene Proteins c-bcl-2/genetics , Amino Acid Sequence , Escherichia coli/genetics , Gene Expression , Membrane Proteins/metabolism , Molecular Sequence Data , Mutation , Proto-Oncogene Proteins c-bcl-2/metabolism , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , bcl-X Protein
11.
J Biol Chem ; 279(28): 28954-60, 2004 Jul 09.
Article in English | MEDLINE | ID: mdl-15123718

ABSTRACT

The proapoptotic Bcl-2 family protein Bid is cleaved by caspase-8 to release the C-terminal fragment tBid, which translocates to the outer mitochondrial membrane and induces massive cytochrome c release and cell death. In this study, we have characterized the conformation of tBid in lipid membrane environments, using NMR and CD spectroscopy with lipid micelle and lipid bilayer samples. In micelles, tBid adopts a unique helical conformation, and the solution NMR (1)H/(15)N HSQC spectra have a single well resolved resonance for each of the protein amide sites. In lipid bilayers, tBid associates with the membrane with its helices parallel to the membrane surface and without trans-membrane helix insertion, and the solid-state NMR (1)H/(15)N polarization inversion with spin exchange at the magic angle spectrum has all of the amide resonances centered at (15)N chemical shift (70-90 ppm) and (1)H-(15)N dipolar coupling (0-5 kHz) frequencies associated with NH bonds parallel to the bilayer surface, with no intensity at frequencies associated with NH bonds in trans-membrane helices. Thus, the cytotoxic activity of tBid at mitochondria may be similar to that observed for antibiotic polypeptides, which bind to the surface of bacterial membranes as amphipathic helices and destabilize the bilayer structure, promoting the leakage of cell contents.


Subject(s)
Apoptosis/physiology , Carrier Proteins/chemistry , Carrier Proteins/metabolism , Cell Membrane/metabolism , Peptide Fragments/chemistry , Protein Conformation , Amino Acid Sequence , BH3 Interacting Domain Death Agonist Protein , Carrier Proteins/genetics , Cell Line , Cell Membrane/chemistry , Circular Dichroism , Humans , Lipid Bilayers/chemistry , Lipid Bilayers/metabolism , Micelles , Mitochondria/metabolism , Molecular Sequence Data , Nuclear Magnetic Resonance, Biomolecular , Peptide Fragments/metabolism , Protein Folding , Proto-Oncogene Proteins c-bcl-2/genetics , Proto-Oncogene Proteins c-bcl-2/metabolism , bcl-X Protein
12.
Magn Reson Chem ; 42(2): 172-9, 2004 Feb.
Article in English | MEDLINE | ID: mdl-14745797

ABSTRACT

Solid-state NMR spectroscopy is being used to determine the structures of membrane proteins involved in the regulation of apoptosis and ion transport. The Bcl-2 family includes pro- and anti-apoptotic proteins that play a major regulatory role in mitochondrion-dependent apoptosis or programmed cell death. The NMR data obtained for (15)N-labeled anti-apoptotic Bcl-xL in lipid bilayers are consistent with membrane association through insertion of the two central hydrophobic alpha-helices that are also required for channel formation and cytoprotective activity. The FXYD family proteins regulate ion flux across membranes, through interaction with the Na(+), K(+)-ATPase, in tissues that perform fluid and solute transport or that are electrically excitable. We have expressed and purified three FXYD family members, Mat8 (mammary tumor protein), CHIF (channel-inducing factor) and PLM (phospholemman), for structure determination by NMR in lipids. The solid-state NMR spectra of Bcl-2 and FXYD proteins, in uniaxially oriented lipid bilayers, give the first view of their membrane-associated architectures.


Subject(s)
Apoptosis/physiology , Lipid Bilayers , Membrane Proteins/chemistry , Proto-Oncogene Proteins c-bcl-2/chemistry , Amino Acid Sequence , Cloning, Molecular , Conserved Sequence , Humans , Magnetic Resonance Spectroscopy/methods , Models, Molecular , Neoplasm Proteins , Protein Conformation , Protein Structure, Secondary , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification
13.
Biochim Biophys Acta ; 1645(1): 15-21, 2003 Jan 31.
Article in English | MEDLINE | ID: mdl-12535606

ABSTRACT

The proteins PLM (phospholemman), CHIF (channel inducing factor), and Mat8 (mammary tumor protein 8 kDa) are members of the FXYD family of ion transport regulatory membrane proteins. Here we describe their cloning and expression in Escherichia coli, and their purification for NMR structural studies in lipid micelles and lipid bilayers. The molecular masses of the purified recombinant FXYD proteins, determined from SDS-PAGE and from MALDI TOF mass spectrometry, reflect monomeric species. The solution NMR and CD spectra in SDS micelles show that they adopt helical conformations. The solid-state NMR spectra in lipid bilayers give the first view of their transmembrane architecture.


Subject(s)
Lipid Bilayers/chemistry , Membrane Proteins/genetics , Neoplasm Proteins/genetics , Potassium Channels/genetics , Amino Acid Sequence , Circular Dichroism , Cloning, Molecular , DNA, Complementary/biosynthesis , DNA, Complementary/chemistry , Electrophoresis, Polyacrylamide Gel , Escherichia coli/metabolism , Intracellular Signaling Peptides and Proteins , Lipids/chemistry , Magnetic Resonance Spectroscopy , Membrane Proteins/biosynthesis , Membrane Proteins/chemistry , Micelles , Molecular Sequence Data , Neoplasm Proteins/biosynthesis , Neoplasm Proteins/chemistry , Potassium Channels/biosynthesis , Potassium Channels/chemistry , Protein Conformation , Sequence Alignment , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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