ABSTRACT
A family with a cross-over between HLA-B and HLA-DR was analysed for its complement alleles. This allowed location of the cross-over between HLA-B and C4. Furthermore, the same family showed a previously undescribed duplication at the C4B locus (C4B* 2,2) that was associated with the HLA-Aw68, Cw8, Bw65, C2*1, Bf*S, C4A*2, DR7, DQw2 haplotype.
Subject(s)
Alleles , HLA-A Antigens/genetics , HLA-B Antigens/genetics , HLA-C Antigens/genetics , Haplotypes , Adult , Child , Crossing Over, Genetic , Electrophoresis , Female , HLA-A Antigens/analysis , HLA-B Antigens/analysis , HLA-C Antigens/analysis , Humans , MaleABSTRACT
A clinical, radiological and immunogenetical study was carried out on 51 Crohn's patients. Rheumatological disorders were found in 16 of them, with higher frequency in those with colon involvement only. A statistically significant increase in the frequencies of HLA-A9 and HLA-Cw3 was noted: Cw3 showed a particularly high frequency in males, and A9 in younger patients. The frequency of HLA-B27 was significantly increased in the patients with colon involvement. In the group of 16 patients with rheumatic diseases HLA antigen frequencies were not significantly different from the control population.
Subject(s)
Arthritis, Rheumatoid/immunology , Crohn Disease/immunology , HLA Antigens/analysis , Adult , Animals , Cats , Colitis/immunology , Female , Humans , Ileitis/immunology , Male , Spondylitis, Ankylosing/immunologyABSTRACT
The Fc-receptor function of the mononuclear phagocyte system was examined in 30 patients affected by multisystem diseases with glomerular involvement by measuring the immune clearance of IgG-sensitized autologous red blood cells in vivo and the immune phagocytosis of monocytes in vitro. Patients studied in the phase of clinically active renal disease showed a significantly reduced Fc-receptor function by both in vivo (p less than 0.001) and in vitro (p = 0.003) assays, as compared to those studied during an inactive phase. Though the nature of the defect remains uncertain, it appears to be related to the active phase of the renal disease as also confirmed by the analysis of individual cases studied longitudinally.
Subject(s)
Cryoglobulinemia/immunology , Glomerulonephritis/immunology , Lupus Erythematosus, Systemic/immunology , Macrophages/immunology , Polyarteritis Nodosa/immunology , Receptors, Fc/immunology , Adolescent , Adult , Aged , Antigen-Antibody Complex/analysis , Female , HLA Antigens/analysis , HLA-D Antigens/analysis , Humans , Male , Middle Aged , Phagocytosis , Spleen/immunologyABSTRACT
Using classical serological methods, we have been able to distinguish anti-HLA-DR from anti-DQ antibodies. The specificity of alloantisera for DQ could be identified on the basis of differential reactivity against B cell and monocyte-enriched leucocyte suspensions and lack of inhibition of cytotoxicity by anti-DR monoclonal antibodies. Some alloantisera, whose reactivity was significantly correlated with DR5, were found to exhibit these characteristics. The suggestion that they recognize DQ specificities in linkage disequilibrium with DR5 was supported by immunochemical analysis.
