Did H1N1 influenza prevention messages reach the vulnerable population along the Mississippi Gulf Coast?

OBJECTIVE: To identify the primary sources of information utilized by a vulnerable population during the 2009 influenza pandemic and examine disease prevention behaviors related to reports of local H1N1 influenza transmission. DESIGN: Cross-sectional study. SETTING: Between May 2009 and December 2009, face-to-face interviews were conducted in towns located in 3 Mississippi counties along the Gulf Coast. PARTICIPANTS: Two hundred sixteen residents of the Mississippi Gulf Coast were interviewed. MAIN OUTCOME MEASURES: Analysis of the interview results described awareness of the influenza outbreak/pandemic and sources of information about the situation. Chi-square tests were used to examine differences in reported disease-preventive behaviors taken by Mississippi Gulf Coast residents before and after H1N1 influenza transmission was confirmed locally. RESULTS: Most subjects were aware of H1N1 influenza at the time of interview (n = 212; 98%). Television (n = 145; 69%), newspaper (n = 40; 19%), and the Internet (n = 19; 9%) were the most common sources of information regarding H1N1 influenza. Hand hygiene (n = 85; 41%) was the most reported preventive measure adopted by study subjects and increased following the confirmation of the first H1N1 influenza cases in Harrison County (χ= 4.46, p= 0.04). CONCLUSIONS: The Centers for Disease Control and Prevention's emphasis on providing health information about H1N1 primarily through the Internet may not have been effective in reaching the public. Provision of health messages through various mediums, especially television, may better inform the public of disease-related prevention messages during a developing influenza pandemic.

Fos after single and repeated self-administration of cocaine and saline in the rat: emphasis on the Basal forebrain and recalibration of expression.

The effects of addictive psychostimulant drugs on the brain change over repeated administrations. We evaluated a large sample of brain structures, particularly ones comprising basal forebrain macrosystems, and determined in which the immediate-early gene product, Fos, is expressed following a single and repeated self-administrations of cocaine. The caudate-putamen and accumbens, comprising the basal ganglia input structures, and the hypothalamic supraoptic and paraventricular nuclei, lateral and medial habenula, mesopontine rostromedial tegmental nucleus and anterior cingulate cortex exhibited Fos expression enhanced by acute self-administration of cocaine (SAC), but desensitized after repeated administrations. Fos expression was mainly enhanced by acutely self-administered cocaine in basal ganglia output and intrinsic structures and the intermediate nucleus of lateral septum, medial division of the central amygdaloid nucleus and zona incerta, but, in contrast, was sensitized in these structures after repeated administrations. Acute and repeated SAC left Fos expression unaffected or marginally enhanced in most extended amygdala structures, of which nearly all, however, exhibited robustly increased Fos expression after repeated saline self-administration, occasionally to levels exceeding those elicited by cocaine. Thus, self-administered cocaine mainly elicits Fos expression, which persists or increases with repeated administrations in some structures, but declines in others. In addition, Fos expression is sensitized in most extended amygdala structures merely by the act of repeated self-administering. Similar spatiotemporal patterns of cocaine- or saline-elicited Fos expression characterize functionally related clusters of structures, such as, eg, basal ganglia input structures, basal ganglia output structures, extended amygdala and structures in the brainstem to which forebrain macrosystems project.

Prominent activation of brainstem and pallidal afferents of the ventral tegmental area by cocaine.

Blockade of monoamine transporters by cocaine should not necessarily lead to certain observed consequences of cocaine administration, including increased firing of ventral mesencephalic dopamine (DA) neurons and accompanying impulse-stimulated release of DA in the forebrain and cortex. Accordingly, we hypothesize that the dopaminergic-activating effect of cocaine requires stimulation of the dopaminergic neurons by afferents of the ventral tegmental area (VTA). We sought to determine if afferents of the VTA are activated following cocaine administration. Rats were injected in the VTA with retrogradely transported Fluoro-Gold and, after 1 week, were allowed to self-administer cocaine or saline via jugular catheters for 2 h on 6 consecutive days. Other rats received a similar amount of investigator-administered cocaine through jugular catheters. Afterward, the rats were killed and the brains processed immunohistochemically for retrogradely transported tracer and Fos, the protein product of the neuronal activation-associated immediate early gene, c-fos. Forebrain neurons exhibiting both Fos and tracer immunoreactivity were enriched in both cocaine groups relative to the controls only in the globus pallidus and ventral pallidum, which, together, represented a minor part of total forebrain retrogradely labeled neurons. In contrast, both modes of cocaine administration strongly increased double-labeling relative to the controls in the brainstem, specifically in the caudal ventromedial mesencephalon and rostromedial pontine tegmentum. It is concluded that a previously unappreciated activation of pallidal and brainstem afferents may contribute to the modulation of dopaminergic neuronal activity following cocaine administration.