ABSTRACT
Neuroinflammation contributes to neuronal degeneration in Parkinson's disease (PD). However, how brain inflammatory factors mediate the progression of neurodegeneration is still poorly understood. Experimental models of PD have shed light on the understanding of this phenomenon, but the exploration of inflammation-driven models is necessary to better characterize this aspect of the disorder. The use of lipopolysaccharide (LPS) to induce a neuroinflammation-mediated neuronal loss is useful to induce reliable elimination of dopaminergic neurons. Nevertheless, how this model parallels the PD-like neuroinflammation is uncertain. In the present work, we used the direct LPS injection as a model inductor to eliminate dopaminergic neurons of the substantia nigra pars compacta (SNpc) in rats and reevaluated the inflammatory reaction. High-resolution 3D histological examination revealed that, although LPS induced a reliable elimination of SNpc dopaminergic neurons, it also generated a massive inflammatory response. This inflammation-mediated injury was characterized by corralling, a damaged parenchyma occupied by a vast population of lesion-associated microglia and macrophages (LAMMs) undertaking wound compaction and scar formation, surrounded by highly reactive astrocytes. LAMMs tiled the entire lesion and engaged in long-standing phagocytic activity to resolve the injury. Additionally, modeling LPS inflammation in a cell culture system helped to understand the role of phagocytosis and cytotoxicity in the initial phases of dopaminergic degeneration and indicated that LAMM-mediated toxicity and phagocytosis coexist during LPS-mediated dopaminergic elimination. However, this type of severe inflammatory-mediated injury, and subsequent resolution appear to be different from the ageing-related PD scenario where the architectural structure of the parenchyma is mostly preserved. Thus, the necessity to explore new experimental models to properly mimic the inflammatory compound observed in PD degeneration.
Subject(s)
Microglia , Parkinson Disease , Animals , Dopamine , Inflammation/metabolism , Lipopolysaccharides/metabolism , Lipopolysaccharides/toxicity , Macrophages/metabolism , Microglia/metabolism , Parkinson Disease/pathology , Phagocytosis , Rats , Substantia Nigra/metabolism , Wound HealingABSTRACT
SERS active nanoparticles were labeled with a reporter molecule and conjugated with anti-EpCAM antibodies. These immuno SERS markers were mixed with leukocytes, MCF-7 breast cancer cells and polystyrene beads, and the mixture was injected into a microfluidic quartz chip. Raman spectra were acquired at 785 nm excitation with 25 milliseconds exposure time in a continuous flow regime. Spectral unmixing by N-FINDR identified spectral signatures of SERS-labelled cells and polystyrene beads. This approach demonstrated rapid and reproducible SERS-assisted cell detection. Strategies are discussed to further increase the throughput for cell sorting.
ABSTRACT
Recently, a simple common-path, two-color interferometer has been used for Doppler-free saturated dispersion spectroscopy of iodine. We have used such a set-up to stabilize a Nd:YAG laser for the first time, to our knowledge. This method requires only a small number of low-cost optical components compared to frequency modulation techniques.We have measured a root Allan variance of 5 . 10(-12) for 0.2 s, and below 5 . 10 (-11) for integration times up to 300 s.
ABSTRACT
We report a master-oscillator fiber power-amplifier system consisting of a diode-pumped monolithic nonplanar ring laser as the master oscillator and a Yb-doped large-mode-area double-clad fiber as the power amplifier. The system emits up to 20.1 W of single-frequency radiation at a wavelength of 1064 nm with diffraction-limited beam quality (M(2)
ABSTRACT
A compact source of stable cw single-frequency radiation at 473 nm has been realized by second-harmonic generation of a diode-pumped miniature Nd:YAG ring laser operating on the (4)F(3/2) - (4)I(9/2) laser transition. By use of potassium niobate (KNbO(3)), single-longitudinal-mode output powers of 500 mW cw with high stability and maximum optical-to-optical conversion efficiencies >81% are achieved. An external semimonolithic cavity permits mode-hop-free frequency tuning of blue radiation over as much as 20 GHz.
ABSTRACT
Applying Cr(4+):YAG saturable absorbers, we demonstrate efficient passively Q -switched operation of diode-pumped Nd:YAG lasers with a maximum average output power of more than 800 mW. Pulse durations below 3 ns, energies of 70 microJ, and peak powers of 30 kW are observed with kilohertz repetition rates. Single-frequency operation is accomplished for all output powers by a nonplanar ring geometry.
ABSTRACT
A compact and efficient source of cw single-frequency radiation at 532 nm with excellent long-term stability has been realized by external frequency doubling of a diode-pumped miniature Nd:YAG ring laser. With a semimonolithic MgO:LiNbO(3) resonator an optical-to-optical conversion efficiency of 89% from 1064 to 532 nm at an output power of 1.1 W was achieved, with a wall plug efficiency of 9%. This system represents an ideal pump source for narrow-linewidth cw optical parametric oscillators with good frequency stability.
ABSTRACT
Amplitude and frequency noise of an injection-locked diode-pumped Nd:YAG ring laser operating at a singlefrequency output power of 20 W cw are investigated. This laser system will be used as a light source for an interferometric gravitational-wave detector.
