Immature Stages, Natural History, Systematics and Conservation of an Endangered Neotropical Butterfly: the Case of Scada karschina delicata (Nymphalidae: Ithomiini).

The endangered butterfly Scada karschina delicata Talbot, 1932 (Nymphalidae: Danainae: Ithomiini) is endemic to northeastern Brazil, occurring in very few forest remnants of the 'Pernambuco Center of Endemism'. Larvae feed on Solanaceae and are very similar to those of other species in the subtribe Mechanitina, with lateral projections on body, one of the main synapomorphies of this subtribe. Based on molecular data, S. k. delicata clustered together with S. karschina karschina, as a monophyletic group sister to the amazon clade of S. reckia. Based on all available data, S. k. delicata is known from only five localities of mid- to high-altitude forests (from 500 to 1000 m of altitude) in northeastern Brazil. Grounded on available data, a new assessment of extinction risk is proposed, and S. k. delicata is now considered Vulnerable (VU) taxon.

A New and Rare Actinote Hübner (Lepidoptera: Nymphalidae: Heliconiinae: Acraeini) from Southeastern Brazil.

The present paper describes a new species of Actinote (Nymphalidae, Heliconiinae, Acraeini), Actinote keithbrowni Freitas, Francini & Mielke sp. nov., from southeastern Brazil, based on morphological and molecular data. The new species is very similar in wing pattern to Actinote eberti, within what we term the "light-gray mimicry complex." The host plants and immature stages of the new species are unknown. Actinote keithbrowni sp. nov. is one of the rarest species of Actinote from Brazil: in total, only eight individuals of this species are known from a narrow region of the Itatiaia massif despite years of collecting effort in that region since the 1940s.

A New Subspecies of Heliconius hermathena (Nymphalidae: Heliconiinae) from Southern Amazonia.

The present paper describes Heliconius hermathena curua Freitas & Ramos ssp. nov. This subspecies exhibits a non-mimetic phenotype typical of H. hermathena, but is characterized by the merging of the yellow streak over the forewing cubitus with the red postmedian band in the dorsal forewing. The subspecies is known from two localities in the south of Altamira, Pará State, Brazil, where it inhabits an isolated patch of "campina" vegetation more than 600 km from the nearest known H. hermathena populations. Geographic isolation of the population is supported by molecular data; based on the mitochondrial gene COI, all individuals of H. hermathena curuassp. nov. form a monophyletic group and all haplotypes found in it are unique, suggesting that gene flow is not currently on-going. Given the fragile situation of Amazonian white sand forests and the proximity of the population to areas of intensive agriculture, this new subspecies and its habitat deserve attention.

Uncovered Diversity of a Predominantly Andean Butterfly Clade in the Brazilian Atlantic Forest: a Revision of the Genus Praepedaliodes Forster (Lepidoptera: Nymphalidae, Satyrinae, Satyrini).

The genus Praepedaliodes Forster, 1964, the only representative of the mega-diverse mostly Andean Pedaliodes complex lineage in the Brazilian Atlantic Forest, is revised. Prior to this study, four species were known, P. phanias (Hewitson, 1862), P. granulata (Butler, 1868), P. amussis (Thieme, 1905) and P. exul (Thieme, 1905). Here, a further six are described, all from SE Brazil, expanding to 10 the number of species in this genus. Lectotypes are designated for P. phanias, P. granulata and P. amussis. The genus is most diverse in the Serra da Mantiqueira (São Paulo, Rio de Janeiro, Minas Gerais) and in the Serra Geral (Paraná, Santa Catarina) with seven species occurring in both ranges. Praepedaliodes phanias is the most widespread species and the only one found in the western part of the Atlantic Forest; only this species and P. duartei Dias, Dolibaina & Pyrcz n. sp. occurring to near sea level. Other species, P. zaccae Dolibaina, Dias & Pyrcz n. sp., P. francinii Freitas & Pyrcz n. sp., P. sequeirae Pyrcz, Dias & Dolbaina n. sp., P. landryi Pyrcz & Freitas n. sp. and P. pawlaki Pyrcz & Boyer n. sp. are strictly montane and the highest species richness is reached at 1400-1800 m. One species, P. sequeirae n. sp., is a narrow endemic found only at timberline in the Agulhas Negras massif above 2300 m. Immature stages are described for two species, P. phanias and P. landryi n. sp. Molecular data (barcode region of cytochrome oxidase, subunit I) and adult morphology, including male and female genitalia, support the genus as monophyletic, belonging to a predominantly Andean clade of the Pedaliodes Butler, 1867 complex. Morphological evidences, in particular female genitalia comparative analysis, indicate the genera Physcopedaliodes Forster, 1964 and Panyapedaliodes Forster, 1964 as possibly the closest relatives to Praepedaliodes. Molecular data are inconclusive in this respect.

A Potentially Endangered New Species of Euptychia Hübner, 1818 (Lepidoptera: Nymphalidae: Satyrinae) from the Atlantic Coastal Forest of Brazil.

A new satyrine species in the subtribe Euptychiina, Euptychia atlantica Nakahara & Freitas sp. nov., is described from the Atlantic coastal forest of Brazil. Based on the existing museum specimens, E. atlantica sp. nov. is known from the coastal montane forests of Rio de Janeiro to south Bahia, a unique biogeographical region which is undergoing rapid degradation. Illustrations of adults and their genitalia, as well as a distribution map, are provided herein, in addition to a discussion of the relationships and conservation status of the new species.

Natural History and Comparative Morphology of Immatures of Gamelia anableps (C. Felder & R. Felder) (Lepidoptera: Saturniidae, Hemileucinae).

Egg, larva, and pupa of Gamelia anableps (C. Felder & R. Felder, 1874) are described for the first time and the chaetotaxy of the first instar provided. Eggs and selected structures of larvae and pupae were also investigated by means of scanning electron microscopy. Eggs are laid in clusters, are ellipsoidal and laterally flat. Larvae passed through eight instars in about 78 days. Pupation occurs inside a poorly organized cocoon of yellowish silk. The dark brown pupa is cylindrical in shape with appendages firmly fused together and to the body wall. The chaeotaxy is compared with other Saturniidae species belongining to Hemileucinae, Ceratocampinae, and Saturniinae and the natural history of G. anableps compared with other Saturniidae.

Graphita gen. nov., a New Genus for Neonympha griphe C. Felder & R. Felder, 1867 (Lepidoptera, Nymphalidae, Satyrinae).

A new genus is described for Neonympha griphe C. Felder & R. Felder, 1867, to contribute toward a revision of the polyphyletic genus Euptychoides Forster, 1964. Based on DNA sequence data, Graphita Nakahara, Marín & Barbosa, gen. nov. is strongly supported as a member of a clade of predominantly southeastern Brazilian taxa, in which it is weakly supported as sister to a well-supported clade containing Pharneuptychia Forster, 1964, Moneuptychia Forster, 1964 and the E. castrensis (Schaus, 1902) species complex. The data show that Graphita griphe comb. nov. is not related to other Euptychoides and not very closely related to any other sampled euptychiines, and thus support the description of this new genus. In addition, we provide morphological illustrations and a distribution map for this taxon based on museum specimens.

Both Palatable and Unpalatable Butterflies Use Bright Colors to Signal Difficulty of Capture to Predators.

Birds are able to recognize and learn to avoid attacking unpalatable, chemically defended butterflies after unpleasant experiences with them. It has also been suggested that birds learn to avoid prey that are efficient at escaping. This, however, remains poorly documented. Here, we argue that butterflies may utilize a variety of escape tactics against insectivorous birds and review evidence that birds avoid attacking butterflies that are hard to catch. We suggest that signaling difficulty of capture to predators is a widespread phenomenon in butterflies, and this ability may not be limited to palatable butterflies. The possibility that both palatable and unpalatable species signal difficulty of capture has not been fully explored, but helps explain the existence of aposematic coloration and escape mimicry in butterflies lacking defensive chemicals. This possibility may also change the role that putative Müllerian and Batesian mimics play in a variety of classical mimicry rings, thus opening new perspectives in the evolution of mimicry in butterflies.

Diversidade e usos de plantas medicinais nos quintais da comunidade de São João da Várzea em Mossoró RN / Diversity and uses of medicinal plants in homegardens at the community São João da Varzea, Mossoró RN

RESUMO Nos quintais do nordeste brasileiro são mantidas tradicionalmente diversas espécies vegetais para o tratamento de doenças. O objetivo do presente trabalho foi realizar um levantamento das espécies medicinais em quintais na Comunidade de São João da Várzea, Mossoró, Rio Grande do Norte, Brasil. Participaram do estudo 22 informantes e foram aplicadas para a coleta de dados técnicas de observação direta e participante, turnê guiada, lista livre e entrevistas semiestruturadas. Verificou-se a presença de 57 espécies pertencentes a 37 famílias botânicas. As espécies com maior frequência de ocorrência foram goiabeira (Psidium guajavaL.) e coqueiro (Cocos nucifera L.), presentes em 59% e 55% dos quintais, respectivamente. Para estas espécies houve maior índice de concordância de uso (CUPc de 61,5% e 92,3%). Foram citadas 67 patologias e o maior número de plantas medicinais foi indicado para o tratamento de gripe, disenteria, inflamações e como cicatrizante de ferimentos. O principal modo de administração foi por via oral, na forma de decocção (58%) e as folhas foram as partes mais usadas (32,6%). Os quintais são sistemas que atuam como uma estratégia de uso mais sustentável dos recursos medicinais por conservarem também as espécies nativas. As espécies medicinais mais frequentes nos quintais são arvores frutífero-alimentícias importantes para a renda familiar. A gripe e desinteria são os principais agravos de saúde tratados com espécies medicinais pela comunidade. Ziziphus joazeiro Mart., Citrus X limonia (L.) Osbeck, Punica granatum L., Cocos nucifera L. e Psidium guajava L. são espécies que merecem maiores estudos farmacológicos.

ABSTRACT Traditionally, at homegardens in the northeastern of Brazil, several plant species are employed for the treatment of diseases. The aim of this study was to research the species of medicinal use in homegardens of São João da Varzea, Mossoró, Rio Grande do Norte, Brazil. 22 homegardens were studied through the techniques of direct and participant observations , guided tours, free list and semi structured interviews. We verified the presence of 57 botanical species belonging to 37 families. The two species with the highest frequency of occurrence were the guava (Psidium guajava L.) and the coconut (Cocos nucifera L.), present in 59% and 55% of the homegardens, respectively. For these species there was more agreement of use (CUPc 61.5% and 92.3%). 67 diseases, including influenza and dysentery were the most common ones mentioned during the interviews. The main mode of administration was orally, in the form of decoction (58%), and the leaves were the most employed component (32.6%). Homegardens are systems that act as a more sustainable use of medicinal resources strategy since they conserve native species. The most common medicinal plants in the homegardens are the fruitful-food trees, important to the family income. The flu and the dysentery are the main health problems treated with medicinal plants by the community. The Ziziphus joazeiro Mart., Citrus X limonia (L.) Osbeck, Punica granatum L., Cocos nucifera L. and Psidium guajava L. are species that deserve further pharmacological studies.

Paulogramma hydarnis (n. comb.) (Nymphalidae: Biblidinae): Distribution, Systematic Position, and Conservation Status of a Rare and Endangered Butterfly.

The nymphalid Paulogramma hydarnis (Godart) (n. comb., previously in the genus Callicore) is an endangered butterfly present in a few montane sites in the Atlantic Forest in the Southeastern Brazil. The precise systematic position of P. hydarnis was previously unknown. Based on molecular data, we find that it is sister to Paulogramma pygas (Godart) (n. comb., also previously in Callicore), a common and widespread species in the Neotropics. In addition, we find that Callicore is not monophyletic and that "Callicore" hydarnis (along with other species) is more related to the genus Paulogramma, and should thus be placed in that genus. The genus Paulogramma is now composed by the following species: Paulogramma pyracmon (Godart), Paulogramma eunomia (Hewitson) n. comb., Paulogramma hydarnis (Godart) n. comb., Paulogramma hystaspes (Fabricius) n. comb., Paulogramma pygas (Godart) n. comb., and Paulogramma tolima (Hewitson, 1852) n. comb. Museum specimens and field data report P. hydarnis in four sites in Southeastern Brazil. Recently, P. hydarnis was recorded for the first time at Parque Nacional do Caparaó, states of Espírito Santo and Minas Gerais, expanding its distribution about 200 km northward of the previously known limit. Although regularly recorded in some sites, most records are historic, before the 1960s, and the current conservation situation of this species is delicate, deserving attention.

Some Possible Cases of Escape Mimicry in Neotropical Butterflies.

The possibility that escape or evasive mimicry evolved in butterflies and other prey insects in a similar fashion to classical Batesian and Müllerian mimicry has long been advanced in the literature. However, there is a general disagreement among lepidopterists and evolutionary biologists on whether or not escape mimicry exists, as well as in which mimicry rings this form of mimicry has evolved. Here, we review some purported cases of escape mimicry in Neotropical butterflies and suggest new mimicry rings involving several species of Archaeoprepona, Prepona, and Doxocopa (the "bright blue bands" ring) and species of Colobura and Hypna (the "creamy bands" ring) where the palatability of butterflies, their ability to escape predator attacks, geographic distribution, relative abundance, and co-occurrence in the same habitats strongly suggest that escape mimicry is involved. In addition, we also indicate other butterfly taxa whose similarities of coloration patterns could be due to escape mimicry and would constitute important case studies for future investigation.

Morphological and molecular marker contributions to disentangling the cryptic Hermeuptychia hermes species complex (Nymphalidae: Satyrinae: Euptychiina).

The genus Hermeuptychia is common and widespread through the Americas, from Argentina to the southern United States of America. All eight recognized species within Hermeuptychia are small and brown, with very similar interspecific external morphologies and intraspecifically variable ocelli patterns that render taxonomic identification based on morphology difficult. In our study, we surveyed variability within Hermeuptychia, and evaluated species boundaries based on molecular data (sequences of the 'barcode' mitochondrial DNA COI gene) and morphology (mainly male genitalia), using a phylogenetic approach. We found eight DNA-based and 12 morphological groups in our sampling. Species names were assigned based mainly on comparisons with male genitalia morphology descriptions corresponding to name-bearing type specimens. Morphological and DNA variability were highly congruent, with the exception of group H, the Hermeuptychia cucullina complex. Also, the barcode region showed a clear threshold for intra- and interspecific mean distances around 2%. Based on these results, we circumscribe the species boundaries in the genus Hermeuptychia and discuss conflicts between mitochondrial genes and classic morphological approaches for identifying and delimiting species. Our study revealed cryptic diversity within an ubiquitous genus of Neotropical butterflies.

Euptychia boulleti (Le Cerf) n. comb. (Lepidoptera: Nymphalidae: Satyrinae), a rare and endangered butterfly from Southeastern Brazil.

This paper discusses the systematic position of the rare and endangered satyrine butterfly Caenoptychia boulleti Le Cerf, the only included species in Caenoptychia (type species), based on adult morphology and molecular data. The results showed that Caenoptychia Le Cerf belongs to the Euptychia Hübner clade, and the genus is synonymized with Euptychia, new synonymy. Euptychia boulleti (Le Cerf) is a new combination. The male genitalia of E. boulleti showed at least one important synapomorphy with the other species of Euptychia, which is the presence of a posterior projection of the tegumen above the uncus. Molecular data reinforces the position of Caenoptychia within the genus Euptychia.

Immature stages of the Brazilian crescent butterfly Ortilia liriope (Cramer) (Lepidoptera: Nymphalidae).

We provide the first information on the morphology of the immature stages (egg, larva, and pupa), oviposition and larval behavior, and host plant, for the Brazilian crescent butterfly Ortilia liriope (Cramer), based on material from Santarém Municipality, Pará State, Northern Brazil. Females of O. liriope lay eggs in clusters. After hatching, larvae eat the exochorion and remain gregarious in all but the final instar. The host plant recorded in the study site is Justicia sp. (Acanthaceae). Despite the scarcity of data on the immature stages of Neotropical Melitaeini, we can already say that some morphological and behavioral traits observed in the immature stages of O. liriope are also present in all known genera in this tribe.

A new genus and species of Euptychiina (Lepidoptera: Nymphalidae: Satyrinae) from southern Brazil.

This paper describes a new genus and a new species of Euptychiina from open grassland habitats (campos de cima da serra) in southern Brazil. The systematic position of this new taxon is discussed based on morphological and molecular data, and it is considered sister to Taydebis Freitas. Since the campos vegetation is considered endangered due to anthropogenic activities, this butterfly species deserves attention and should be included in future conservation plans for this biome.

From the phylogeny of the Satyrinae butterflies to the systematics of Euptychiina (lepidoptera: nymphalidae): history, progress and prospects.

We review the various proposals of evolutionary and classification schemes for Satyrinae and particularly Euptychiina butterflies, assessing progress and prospects of research for the group. Among the highlights is the proposal to include Morphini, Brassolini and Amathusiini as part of Satyrinae. Although it is clear that this hypothesis requires further investigation, phylogenetic studies recently conducted recover this clade as part of Satyrinae with high support. The phylogenetic analyses for Euptychiina carried out to date recover the monophyly of the group and have identified a variety of genera as non-monophyletic. Further work is necessary to resolve the position of the subtribe and the evolutionary relationships of several genera.

The endangered butterfly Charonias theano (Boisduval) (Lepidoptera: Pieridae): current status, threats and its rediscovery in the state of São Paulo, southeastern Brazil.

The pierid Charonias theano (Boisduval), an endangered butterfly species, has been rarely observed in nature, and has not been recorded in the state of São Paulo in the last 50 years despite numerous efforts to locate extant colonies. Based on museum specimens and personal information, C. theano was known from 26 sites in southeastern and southern Brazil. Recently, an apparently viable population was recorded in a new locality, at Serra do Japi, Jundiaí, São Paulo, with several individuals observed during two weeks in April, 2011. The existence of this population at Serra do Japi is an important finding, since this site represents one of the few large forested protected areas where the species could potentially persist not only in the state of São Paulo, but within its entire historical distribution.

Out of the Andes: patterns of diversification in clearwing butterflies.

Global biodiversity peaks in the tropical forests of the Andes, a striking geological feature that has likely been instrumental in generating biodiversity by providing opportunities for both vicariant and ecological speciation. However, the role of these mountains in the diversification of insects, which dominate biodiversity, has been poorly explored using phylogenetic methods. Here we study the role of the Andes in the evolution of a diverse Neotropical insect group, the clearwing butterflies. We used dated species-level phylogenies to investigate the time course of speciation and to infer ancestral elevation ranges for two diverse genera. We show that both genera likely originated at middle elevations in the Andes in the Middle Miocene, contrasting with most published results in vertebrates that point to a lowland origin. Although we detected a signature of vicariance caused by the uplift of the Andes at the Miocene-Pliocene boundary, most sister species were parapatric without any obvious vicariant barrier. Combined with an overall decelerating speciation rate, these results suggest an important role for ecological speciation and adaptive radiation, rather than simple vicariance.

New evidence on the systematic and phylogenetic position of Parides burchellanus (Lepidoptera: Papilionidae).

Parides burchellanus is considered a rare and threatened swallowtail species restricted to central Brazil. It shows considerable morphological similarity to Parides panthonus jaguarae, with which it shares both geographical range and larval host plant. At present, P. burchellanus and P. panthonus are believed to be distinct species, based on minor differences in male genitalia. In this study, the phylogenetic and systematic position of P. burchellanus in relation to three subspecies of P. panthonus (P. p. jaguarae, P. p. lysimachus and P. p. aglaope) was evaluated using molecular evidence: the complete sequence of the mtDNA genes COI and COII and of the nuclear gene EF-1α (c. 3300 bp). In addition, the informativeness of the 'barcode' region next to the 5' end of COI (c. 650 bp) was evaluated for delimiting these taxa. Individual analysis by neighbour-joining, using Kimura 2-parameter distance model, and by maximum parsimony showed that P. p. jaguarae + P. p. lysimachus + P. p. aglaope + P. burchellanus form a strongly supported monophyletic clade, and all molecular regions consistently recovered P. p. jaguarae and P. burchellanus as sister species. The genetic divergence among the subspecies of P. panthonus and P. burchellanus is equivalent to the divergence among conspecifics of other species of Parides, and smaller than the interspecific divergence among different sister species of this genus. The results support the proposal that P. p. jaguarae and P. burchellanus are likely to be synonymous, and suggest that P. burchellanus can be considered conspecific with P. panthonus. The taxonomic classification of P. burchellanus should be revised on the basis of the molecular data.

Population biology of Parides anchises nephalion (Papilionidae) in a coastal site in Southeast Brazil

A population of Parides anchises nephalion was studied during seven months in SE Brazil. The population size was about 10-20 individuals (with theoretic maximum near 60 individuals), with small variations in some months. Sex ratio was male biased, with males dominating in all months. The age structure was not stable, with an increase in new individuals before the population peak in December. The residence time was 14.1 8.2 days for males and 9.0 3.6 to females, with the maximum registered of 30 days. Males can travel distances of up to 400 m, but most individuals were always recaptured in the same site. The mean forewing length was greater in females. The population features agree with those found in other species of Parides in other neotropical sites