Directional field-induced metallization of quasi-one-dimensional Li0.9Mo6O17.

We report a detailed magnetotransport study of the highly anisotropic quasi-one-dimensional oxide Li(0.9)Mo(6)O(17) whose in-chain electrical resistivity diverges below a temperature T_{min} approximately 25 K. For T < T_{min}, a magnetic field applied parallel to the conducting chain induces a large negative magnetoresistance and, ultimately, the recovery of a metallic state. We show evidence that this insulator-metal crossover is a consequence of field-induced suppression of a density-wave gap in a highly one-dimensional conductor. At the highest fields studied, there is evidence for the possible emergence of a novel superconducting state with an onset temperature T_{c} > 10 K.

Correlation between the superconducting transition temperature and anisotropic quasiparticle scattering in Tl2Ba2CuO6+delta.

Angle-dependent magnetoresistance measurements are used to determine the isotropic and anisotropic components of the transport scattering rate in overdoped Tl2Ba2CuO6+delta for a range of Tc values between 15 and 35 K. The size of the anisotropic scattering term is found to scale linearly with Tc, establishing a link between the superconducting and normal state physics. A comparison with results from angle resolved photoemission spectroscopy indicates that the transport and quasiparticle lifetimes are distinct.

Chondrogenic differentiation of adult dermal fibroblasts.

Cell sources for generation of articular cartilage ex vivo are limited. To explore options other than stem cells, dermal fibroblasts were tested for their developmental potential when cultured on the cartilage matrix proteoglycan, aggrecan. A previous study suggested such an effort would be successful (M. M. French et al., Journal of Cell Biology 145:1103-1115, 1999). The adult dermal fibroblast cell line, RAB-9, was used in these assays. While initial attempts to differentiate the cells were unsuccessful, after pretreatment with insulin growth factor one (IGF-I), the cells were able to differentiate in culture on aggrecan. After 24 h in culture on aggrecan, the majority of the cells formed dense aggregates reminiscent of condensing mesenchymal cells in development. At 1 week, these aggregates stained positively with both Safranin O and antibodies against collagen type II. This staining was maintained through the conclusion of the experiment at week 4. RT-PCR for collagen II supports the hypothesis that dermal fibroblasts can be triggered to differentiate by culture on cartilage matrix proteoglycans. A three-fold increase in collagen type II mRNA expression is seen when cells are cultured on aggrecan in comparison to controls. These results provide an initial step towards a cell source that may prove equally successful for the generation of cartilage in the laboratory.

Expression of the heparan sulfate proteoglycan, perlecan, during mouse embryogenesis and perlecan chondrogenic activity in vitro.

Expression of the basement membrane heparan sulfate proteoglycan (HSPG), perlecan (Pln), mRNA, and protein has been examined during murine development. Both Pln mRNA and protein are highly expressed in cartilaginous regions of developing mouse embryos, but not in areas of membranous bone formation. Initially detected at low levels in precartilaginous areas of d 12.5 embryos, Pln protein accumulates in these regions through d 15.5 at which time high levels are detected in the cartilage primordia. Laminin and collagen type IV, other basal lamina proteins commonly found colocalized with Pln, are absent from the cartilage primordia. Accumulation of Pln mRNA, detected by in situ hybridization, was increased in d 14.5 embryos. Cartilage primordia expression decreased to levels similar to that of the surrounding tissue at d 15.5. Pln accumulation in developing cartilage is preceded by that of collagen type II. To gain insight into Pln function in chondrogenesis, an assay was developed to assess the potential inductive activity of Pln using multipotential 10T1/2 murine embryonic fibroblast cells. Culture on Pln, but not on a variety of other matrices, stimulated extensive formation of dense nodules reminiscent of embryonic cartilaginous condensations. These nodules stained intensely with Alcian blue and collagen type II antibodies. mRNA encoding chondrocyte markers including collagen type II, aggrecan, and Pln was elevated in 10T1/2 cells cultured on Pln. Human chondrocytes that otherwise rapidly dedifferentiate during in vitro culture also formed nodules and expressed high levels of chondrocytic marker proteins when cultured on Pln. Collectively, these studies demonstrate that Pln is not only a marker of chondrogenesis, but also strongly potentiates chondrogenic differentiation in vitro.

Expression of heparan sulfate proteoglycan (perlecan) in the mouse blastocyst is regulated during normal and delayed implantation.

Previous studies have shown that expression of the heparan sulfate proteoglycan, perlecan, on the external trophectodermal cell surfaces of mouse blastocysts increases during acquisition of attachment competence. However, it is not clear if this change in perlecan protein expression also is reflected at the level of perlecan mRNA expression. In the present investigation, the spatial and temporal patterns of perlecan mRNA expression in the mouse embryo during the periimplantation period were examined by in situ hybridization and reverse transcriptase-polymerase chain reaction. In addition, a delayed implantation model was used to determine the expression of perlecan mRNA and protein in dormant and estrogen-activated hatched blastocysts. The results demonstrate that perlecan mRNA expression is low in morulae, but increases in Day 4 blastocysts, attaining maximal expression in Day 4.5 attachment-competent blastocysts. In contrast, perlecan mRNA is detected in both the dormant and estrogen-activated delayed blastocysts; however, within 12 hr of blastocyst activation by estrogen, both perlecan protein and heparan sulfate chain expression markedly increase. Taken together, these results suggest that during normal development perlecan mRNA expression increases with the acquisition of attachment competence. Moreover, perlecan protein expression also is attenuated during delayed implantation and appears to increase in response to nidatory estrogen, perhaps via the increased translation of preexisting perlecan mRNA.