Wetland butterfly thriving in abandoned jungle: Neptis rivularis in the Czech Republic.

With ongoing insect declines, species expanding in distribution and abundance deserve attention, as understanding their success may help design conservation strategies for less successful species. Common causes of these successes include warmer climates, novel resources, and exploiting land use change, including land abandonment. These factors affect the nymphalid butterfly Neptis rivularis, developing on Spiraea spp. shrubs and reaching the north-western limits of its trans-Palearctic distribution in Central Europe. We combined mark-recapture, behaviour analysis, and distribution modelling to study N. rivularis in wetlands of the Trebonsko Protected Landscape (IUCN category V). The long-living adults (up to 4 weeks) spent a considerable amount of time searching for partners, ovipositing and nectaring at Spiraea shrubs, alternating this with stays in tree crowns, where they located cool shelters, spent nights, and presumably fed on honeydew. They formed high-density populations (310 adults/ha), exploiting high host plant abundance. They adhered to floodplains and to conditions of relatively mild winters. The ongoing Spiraea encroachment of abandoned alluvial grasslands is, thus, a transient situation, ultimately followed by forest encroachment. Rewilding the habitats by introducing native ungulates presents an opportunity to restore the disturbance regime of the sites. The increased resource supply combined with a warming climate has opened up temperate Europe to colonization by N. rivularis.

Five new species of the genus Meganaclia Aurivillius, 1892 (Lepidoptera: Erebidae: Arctiinae: Syntomini).

The Afrotropical genus Meganaclia Aurivillius, 1892 is revised, five new species M. smithi sp. n., M. grehani sp. n., M. josephi sp. n., M. johannae sp. n. and M. gaerberfesti sp. n. are described with a key for identification of species based on male genitalia structures. Habitus and genitalia of all taxa are illustrated and a distribution map is provided.

More hidden diversity in a cryptic species complex: a new subspecies of Leptideasinapis (Lepidoptera, Pieridae) from Northern Iran.

A new subspecies of Leptideasinapis from Northern Iran, discovered by means of DNA barcoding, is described as Leptideasinapistabarestanassp. nov. The new subspecies is allopatric with respect to other populations of L.sinapis and is genetically distinct, appearing as a well-supported sister clade to all other populations in COI-based phylogenetic reconstructions. Details on karyotype, genitalia, ecology and behaviour for the new subspecies are given and a biogeographical speciation scenario is proposed.

Geography of Indian Butterflies: Patterns Revealed by Checklists of Federal States.

Butterflies are widely used to analyze biogeographical patterns, both at the global and regional scales. Thus far, most of the latter originated from well-surveyed northern regions, while the species-rich tropical areas lag due to a lack of appropriate data. We used checklists of 1379 butterfly species recorded in 36 federal states of the Republic of India (1) to explore the basic macroecological rules, and (2) to relate species richness and the distribution of endemics and geographic elements to geography, climate, land covers and socioeconomic conditions of the states. The area, land covers diversity and latitude did not affect species richness, whereas topographic diversity and the precipitation/temperature ratio (energy availability) were positive predictors. This is due the geographic and climatic idiosyncrasies of the Indian subcontinent, with its highest species richness in the small, densely forested mountainous northeast that receives summer monsoons. The peninsular effect that decreases the richness towards the tip of subcontinent is counterbalanced by the mountainous forested Western Ghats. Afrotropical elements are associated with savannahs, while Palearctic elements are associated with treeless habitats. The bulk of Indian butterfly richness, and the highest conservation priorities, overlap with global biodiversity hotspots, but the mountainous states of the Western Himalayas and the savannah states of peninsular India host distinctive faunas.

Perishing rich, expanding poor: Demography and population genetic patterns in two congeneric butterflies.

In human-altered landscapes, specialist butterflies typically form spatially restricted populations, genetically differentiated due to dispersal restrictions. Generalists, in contrast, display minimum differentiation but high genetic diversity. While local-level actions suffice to conserve specialists and landscape-level actions are necessary for generalists, minimum information exists regarding conservation of species with intermediate features. We targeted two congeneric butterflies, the recently re-expanding Argynnis adippe and the strongly declining A. niobe, co-occurring in the pastoral landscape of the Carpathian Mountains, Czech Republic. We integrated species distribution models, mark-recapture and microsatellite analysis to compare their habitat requirements, adult demography, dispersal and genetic patterns, and expanded the genetic analysis across the Carpathian Arc and beyond to delimit spatial conservation units. In two mountain valleys, both species formed interconnected populations numbering thousands of individuals. Mobility patterns suggested the populations' interconnection across the Czech Carpathians. Genetic diversity was extremely poor in the nonthreatened A. adippe and moderate in the declining A. niobe. No population differentiation was detected within the Czech Carpathians (~1500 km2 ). Low genetic diversity and no differentiation was preserved in A. adippe across East Central Europe, whereas in A. niobe, populations from Serbia were differentiated from the Carpathian Arc + Alps. The high adult mobility linked to low differentiation probably reflects the distribution of larval resources, historically widespread but sparse and currently declining for A. niobe (grazing-disturbed grounds), while currently increasing for A. adippe (abandonment scrub, disturbed woodlands). Units as large as entire mountain systems define population boundaries, and hence conservation management units, for both species.

Microhabitat Selection for Overwintering: Overwintering Conditions of Three Jumping Spiders (Pellenes tripunctatus, P. nigrociliatus, and Attulus penicillatus) Living in Terrestrial Shells in the Czech Republic.

Taking the regular overwintering of spider species in land snail shells as a model, we studied environmental conditions affecting the choice of overwintering sites in three jumping spider species: Pellenes tripunctatus, Pellenes nigrociliatus, and Attulus penicillatus. The research was conducted at 11 steppe localities on calcareous bedrock with abundant empty shells (mainly Caucasotachea vindobonensis and Xerolenta obvia). We documented 889 shells and collected 186 of them, of which 113 were inhabited by 146 spider individuals (13 species). Our three focal species made up 81.5% of these. We found different environmental preferences between the sexes in P. tripunctatus and P. nigrociliatus. These females preferred shells with more vegetation nearby. In the case of P. tripunctatus, these were shells with a higher proportion of herbs, whereas P. nigrociliatus selected for a higher proportion of moss. In the immediate vicinity of the shells, environmental conditions did not differ significantly. We found insufficient A. penicillatus to determine any preferences. We also recorded six overwintering P. tripunctatus individuals in a single shell (in environmental conditions preferred by females), consisting of five females and one male, which indicated an unusual social behavior for these spider species.

New and less known Notodontidae from Manipur, India (Lepidoptera: Noctuoidea).

The present study discusses the systematic accounts of 66 species of the family Notodontidae recorded from the Indian state of Manipur. There are some records of the moths from other places including Sikkim (India) and Nepal in addition to Shirui Hill, Manipur. Here, we report, (1) three new species to science viz. Cleapa ukhrulensis Irungbam & Schintlmeister sp. nov., Spatalina rimbiensis Irungbam & Schintlmeister sp. nov. and Hexafrenum tangkhula Irungbam & Schintlmeister sp. nov.; (2) seventeen species for the first time from India: Hupodonta corticalis Butler, 1877, Chalepa binotata Kiriakoff, 1959, Besaia isis Schintlmeister, 1997, Ogulina. argentilinea Cai, 1982, Bireta juncturina (Kiriakoff, 1959), Spatalina melanopa Schintlmeister, 2007, S. desiccata desiccata (Kiriakoff, 1963), S. umbrosa (Leech, 1898), Phalera albocalceolata (Bryk, 1950), Neodrymonia albinomarginata Schintlmeister, 2007, Syntypistis wunna (Schintlmeister, 1997), S. scensus (Schintlmeister, 1997), Harpyia nadiae Morozov, 2013, Hexafrenum viola (Schintlmeister, 1997), Rachiades lichenicolor siamensis Sugi, 1993, Pheosiopsis norina Schintlmeister, 1989, and Periphalera spadixa Wu & Fang, 2003; and (3) one genus for the first time from India: Rachiades Kiriakoff, 1967. Thus, the present study updates the Indian Notodontidae richness to a total of 281 species (including nominotypical subspecies) and 16 subspecies under 120 genera. Here, we provide species diagnoses along with habitus, and genitalia images for each of these newly recorded species.

AN INVENTORY OF THE BUTTERFLIES OF MANIPUR, INDIA (INSECTA: LEPIDOPTERA).

The butterfly fauna of Manipur is poorly known, and a few sporadic studies were carried out decades ago. In this study, butterflies were photographed and/or collected in 80 localities including revisiting of 12 historical localities in the hills and valleys from Manipur state, India. Butterflies were regularly sampled between 2010 and 2019. In the present checklist, we have included both previously published and recently recorded species: 798 species belong to six families; Papilionidae (52 sp.), Pieridae (39 sp.), Riodinidae (17 sp.), Lycaenidae (225 sp.), Nymphalidae (270 sp.), and Hesperiidae (195 sp.). Eight species were rediscovered during the study; Byasa latreillei kabrua, Papilio machaon suroia, Lamproptera meges indistincta, Bhutanitis lidderdalii lidderdalii, Lethe kangjupkula, Una usta usta, Arhopala hellenore hellenore and Celaenorrhinus munda maculicornis. Thirty-two species were new records to Manipur; Papilio agestor agestor, Lamproptera curius curius, Appias albina darada, Artogeia erutae montana, Miletus mallus, Flos fulgida fulgida, Cigaritis nipalicus, Rapala rectivitta, Heliophorus kohimensis, H. tamu, Jamides caeruleus, Tarucus venosus, Everes huegelii dipora, Talicada nyseus nyseus, Lestranicus transpectus, Euploea radamanthus radamanthus, Lethe distans, L. dura gammiei, L. latiaris latiaris, L. sura, Neope pulaha, N. yama yama, Zipaetis scylax, Algia fasciata fasciata, Athyma opaline, Pantoporia paraka paraka, Kallima knyvettii, Celaenorrhinus asmara, Hyarotis adrastus praba, Erionota torus, Baoris penicillata chapmani, and Potanthus mingo ajax of which, Miletus mallus was a new record for India. Ninety species are legally protected in schedules (I, II IV) of Indian (Wildlife) Protection Act, 1972.

Cross-continental phylogeography of two Holarctic Nymphalid butterflies, Boloria eunomia and Boloria selene.

Pleistocene glaciations had significant effects on the distribution and evolution of species inhabiting the Holarctic region. Phylogeographic studies concerning the entire region are still rare. Here, we compared global phylogeographic patterns of one boreo-montane and one boreo-temperate butterflies with largely overlapping distribution ranges across the Northern Hemisphere, but with different levels of range fragmentation and food specialization. We reconstructed the global phylogeographic history of the boreo-montane specialist Boloria eunomia (n = 223) and of the boreo-temperate generalist Boloria selene (n = 106) based on mitochondrial and nuclear DNA markers, and with species distribution modelling (SDM). According to the genetic structures obtained, both species show a Siberian origin and considerable split among populations from Nearctic and Palaearctic regions. According to SDMs and molecular data, both butterflies could inhabit vast areas during the moderate glacials. In the case of B. selene, high haplotype diversity and low geographic structure suggest long-lasting interconnected gene flow among populations. A stronger geographic structuring between populations was identified in the specialist B. eunomia, presumably due to the less widespread, heterogeneously distributed food resources, associated with cooler and more humid climatic conditions. Populations of both species show opposite patterns across major parts of North America and in the case of B. eunomia also across Asia. Our data underline the relevance to cover entire distribution ranges to reconstruct the correct phylogeographic history of species.

Functional characterization of ecto-5'-nucleotidases and apyrases in Drosophila melanogaster.

Ecto-5'-nucleotidases are glycosyl phosphatidylinositol (GPI)-linked membrane-bound glycoproteins that convert extracellular AMP to adenosine. They play important roles in the inflammatory response where they modulate levels of pro-inflammatory extracellular ATP and anti-inflammatory extracellular adenosine. They are found in the saliva of blood feeding insects and also have a role in male reproduction. Drosophila possesses five genes with eight alternative transcripts encoding proteins with sequence homology to mammalian ecto-5'-nucleotidases. Here we show that two of them - NT5E-1 (CG4827) and NT5E-2 (CG30104) - are GPI-linked proteins with ecto-5'-nucleotidase activity but that they can also be released from the GPI anchor and exhibit secreted 5'-nucleotidase activity in growth media. The third locus in the cluster, CG30103, most likely also encodes a GPI-anchored membrane-bound protein but without 5'-nucleotidase activity, possibly due to the numerous substitutions in the amino acid sequence. Together with NT5E-2, CG30103 is also expressed in the testis offering an interesting model to investigate ecto-5'-nucleotidase enzymatic and extra-enzymatic function in male reproduction. CG42249 locus encoding two alternative transcripts is sequentially similar to family of apyrases related to 5'-nucleotidases and we show here that together with CG5276 belonging to another family of calcium-activated nucleotidases function as apyrases converting extracellular ATP to ADP and AMP. The last locus, CG11883, encodes most likely a cytoplasmic/mitochondrial protein.