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1.
Sci Rep ; 10(1): 12409, 2020 07 24.
Article in English | MEDLINE | ID: mdl-32710087

ABSTRACT

Vascular endothelial growth factor-A (VEGF) is the angiogenic factor promoting the pathological neovascularization in age-related macular degeneration (AMD) or diabetic macular edema (DME). Evidences have suggested a neurotrophic and neuroprotective role of VEGF, albeit in retina, cellular mechanisms underlying the VEGF neuroprotection remain elusive. Using purified adult retinal ganglion cells (RGCs) in culture, we demonstrated here that VEGF is released by RGCs themselves to promote their own survival, while VEGF neutralization by specific antibodies or traps drastically reduced the RGC survival. These results indicate an autocrine VEGF neuroprotection on RGCs. In parallel, VEGF produced by mixed retinal cells or by mesenchymal stem cells exerted a paracrine neuroprotection on RGCs. Such neuroprotective effect was obtained using the recombinant VEGF-B, suggesting the involvement of VEGF-R1 pathway in VEGF-elicited RGC survival. Finally, glaucomatous patients injected with VEGF traps (ranibizumab or aflibercept) due to either AMD or DME comorbidity, showed a significant reduction of RGC axon fiber layer thickness, consistent with the plausible reduction of the VEGF autocrine stimulation of RGCs. Our results provide evidence of the autocrine neuroprotective function of VEGF on RGCs is crucially involved to preserve injured RGCs such as in glaucomatous patients.


Subject(s)
Glaucoma/drug therapy , Retinal Ganglion Cells/metabolism , Vascular Endothelial Growth Factor A/metabolism , Aged , Aged, 80 and over , Animals , Autocrine Communication/drug effects , Cell Survival/drug effects , Cells, Cultured , Diabetic Retinopathy/complications , Diabetic Retinopathy/drug therapy , Female , Glaucoma/etiology , Glaucoma/pathology , Humans , Intravitreal Injections , Macular Degeneration/complications , Macular Degeneration/drug therapy , Macular Edema/complications , Macular Edema/drug therapy , Male , Mesenchymal Stem Cells/metabolism , Middle Aged , Paracrine Communication/drug effects , Primary Cell Culture , Prospective Studies , Ranibizumab/administration & dosage , Rats , Receptors, Vascular Endothelial Growth Factor/administration & dosage , Recombinant Fusion Proteins/administration & dosage , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Retinal Ganglion Cells/drug effects , Retinal Ganglion Cells/pathology , Vascular Endothelial Growth Factor A/antagonists & inhibitors , Vascular Endothelial Growth Factor B/genetics , Vascular Endothelial Growth Factor B/metabolism , Vascular Endothelial Growth Factor Receptor-1/antagonists & inhibitors , Vascular Endothelial Growth Factor Receptor-1/metabolism
2.
Biol Aujourdhui ; 213(3-4): 131-140, 2019.
Article in French | MEDLINE | ID: mdl-31829933

ABSTRACT

Discovered in the eighties by Pr Baulieu and colleagues, neurosteroids are a class of neuroactive brain-born steroids, which comprises the steroid hormones, their biosynthesis precursors and their metabolites. They can act through genomic as well as non-genomic pathways. Genomic pathways, only triggered by the neurosteroid hormones, are, in the brain, the same as those largely described in the periphery: the binding of these steroid hormones to nuclear receptors leads to transcription regulations. On the other hand, their precursors and metabolites, such as pregnenolone (PREG), dehydroepiandrosterone (DHEA), their respective sulfate esters, pregnenolone sulfate (PREG-S) and DHEA sulfate (DHEA-S) and allopregnanolone (ALLOP), are defined as neurosteroids, but no corresponding nuclear receptors have been identified so far. In fact, they trigger non-genomic pathways which consist in (i) inhibitory ionotropic receptors, (ii) excitatory ionotropic receptors and (iii) the microtubular system. Hence, inhibitory neurosteroids, whose mostly studied representative is ALLOP, positively modulate, or directly activate, the ionotropic GABA-A receptors. In contrast, excitatory neurosteroids, represented by PREG-S, DHEA-S and DHEA, inhibit the GABA-A receptors, and activate, directly or indirectly, through the sigma-1 receptors, the NMDA glutamate receptors. Neurosteroids of the third group, the microtubular neurosteroids, are able to bind microtubule associated proteins, in particular MAP2, to promote microtubule assembly, neurite outgrowth and in fine structural neuroplasticity. So far, PREG, DHEA and progesterone are the three identified microtubular neurosteroids. The pharmacological properties of neurosteroids have led to specific investigations for assessing their therapeutic potentialities in psychiatric diseases, using validated animal models. In some cases, clinical trials were also performed. These studies showed that ALLOP, the main inhibitory neurosteroid, displayed clear-cut anxiolytic-like and antidepressant-like efficacy in animals. It has been subsequently developed as Brexanolone and tested with success in phase III of clinical trials for the treatment of post-partum depression. Although showing pro-cognitive properties in animals, the sulfated neurosteroids, PREG-S and DHEA-S, were, in contrast, never tested in clinical trials, probably due to their poor stability and proconvulsivant side effects. Their respective non-sulfated forms, PREG and DHEA, showed antidepressant and antipsychotic efficacies in clinical trials, but these drugs never reached the phase III of clinical development because their therapeutic uses would have led to an overproduction of active metabolites responsible for intolerable side effects. The alternative strategy which has been selected consists of the development of non-metabolizable synthetic derivatives of these natural steroids, which keep the same neuroactive properties as their parent molecules, but are devoid of any hormonal side effects. An example of such innovative drugs is MAP4343, a synthetic derivative of PREG, which exhibits potent antidepressant-like efficacy in validated animal models. It is currently tested in depressed patients.


TITLE: Potentialités thérapeutiques des neurostéroïdes en psychiatrie. ABSTRACT: Les neurostéroïdes constituent une famille de molécules synthétisées par le cerveau, représentée par les hormones stéroïdes elles-mêmes, mais également par certains de leurs précurseurs et métabolites. Ils ont des propriétés neuroactives en stimulant des voies de signalisation non génomiques, spécifiques des neurones. Trois types de neurostéroïdes ont été identifiés selon les voies qu'ils activent, à savoir (i) les neurostéroïdes inhibiteurs, (ii) les neurostéroïdes excitateurs et (iii) les neurostéroïdes microtubulaires. Les neurostéroïdes inhibiteurs activent les récepteurs ionotropiques GABA-A, tandis que les neurostéroïdes excitateurs inhibent les courants GABAergiques et stimulent la neurotransmission glutamatergique (soit directement en activant les récepteurs NMDA, soit indirectement via la stimulation des récepteurs sigma-1). Enfin, les neurostéroïdes microtubulaires sont capables de se lier aux protéines associées aux microtubules, comme MAP2, pour favoriser la croissance des microtubules, et in fine la plasticité neuronale. En regard de leurs actions pharmacologiques, certains neurostéroïdes ont fait l'objet d'études cliniques pour le traitement de maladies psychiatriques. C'est le cas de l'alloprégnanolone, le principal neurostéroïde inhibiteur, qui a montré une efficacité dans le traitement de la dépression du post-partum et de l'anxiété. Contrairement à leurs dérivés sulfatés qui n'ont jamais été testés en clinique, la DHEA (déhydroépiandrostérone) et la prégnénolone ont montré des effets antidépresseurs et antipsychotiques. Cependant, la surproduction éventuelle d'hormones provoquée par leur métabolisation a conduit à développer des dérivés de synthèse non métabolisables. C'est le cas du composé MAP4343, un dérivé de la prégnénolone, qui a montré des effets de type antidépresseur dans différents modèles animaux. Il fait actuellement l'objet d'un développement clinique pour le traitement de la dépression.


Subject(s)
Mental Disorders/drug therapy , Neurosteroids/therapeutic use , Psychiatry/trends , Animals , Dehydroepiandrosterone/pharmacology , Dehydroepiandrosterone Sulfate/pharmacology , Depression/drug therapy , Drugs, Investigational/therapeutic use , Humans , Neuronal Plasticity/drug effects , Pregnanolone/pharmacology , Signal Transduction/drug effects , Signal Transduction/genetics , Therapies, Investigational/methods , Therapies, Investigational/trends
3.
Pharmacol Ther ; 191: 190-206, 2018 11.
Article in English | MEDLINE | ID: mdl-29953900

ABSTRACT

Neurosteroids are neuroactive brain-born steroids. They can act through non-genomic and/or through genomic pathways. Genomic pathways are largely described for steroid hormones: the binding to nuclear receptors leads to transcription regulation. Pregnenolone, Dehydroepiandrosterone, their respective sulfate esters and Allopregnanolone have no corresponding nuclear receptor identified so far whereas some of their non-genomic targets have been identified. Neuroplasticity is the capacity that neuronal networks have to change their structure and function in response to biological and/or environmental signals; it is regulated by several mechanisms, including those that involve neurosteroids. In this review, after a description of their biosynthesis, the effects of Pregnenolone, Dehydroepiandrosterone, their respective sulfate esters and Allopregnanolone on their targets will be exposed. We then shall highlight that neurosteroids, by acting on these targets, can regulate neurogenesis, structural and functional plasticity. Finally, we will discuss the therapeutic potential of neurosteroids in the pathophysiology of neurological diseases in which alterations of neuroplasticity are associated with changes in neurosteroid levels.


Subject(s)
Nervous System Diseases/physiopathology , Neuronal Plasticity/physiology , Neurotransmitter Agents/metabolism , Animals , Dehydroepiandrosterone/biosynthesis , Dehydroepiandrosterone/metabolism , Humans , Nervous System Diseases/therapy , Neurogenesis/physiology , Neurotransmitter Agents/biosynthesis , Pregnanolone/biosynthesis , Pregnanolone/metabolism , Pregnenolone/biosynthesis , Pregnenolone/metabolism
4.
Adv Exp Med Biol ; 975 Pt 2: 687-701, 2017.
Article in English | MEDLINE | ID: mdl-28849492

ABSTRACT

Retinal ganglion cell (RGC) degeneration occurs in numerous retinal diseases, either as a primary process like in glaucoma, or secondary to photoreceptor loss and no efficient compound targeting directly RGC neuroprotection is yet available. We previously described that taurine exerts a direct protective effect on RGCs cultured under serum-deprived conditions. Because taurine was known to have an agonist-like activity for GABA/glycine receptors, we investigated here if the taurine-elicited neuroprotective effect may be mediated through the activation of these receptors using selective antagonist ligands. RGCs were purified, seeded in 96-well plate and maintained in culture during 6 days in vitro. Viable cells were labelled with calcein and densities in full-well area were then automatically counted. Here we show that the protective effect of taurine against RGC loss observed under serum deprivation can be mediated through the GABAB receptor stimulation. Hence, two selective agonists, including baclofen, at this metabotropic GABAB receptor were found to reproduce taurine action by enhancing RGC survival in culture. This study suggests that GABAB receptor stimulation provides direct neuroprotection for RGCs. Accordingly, drugs targeting GABAB receptor may represent a new way for the prevention of RGC degeneration.


Subject(s)
GABA-B Receptor Agonists/pharmacology , Neuroprotective Agents/pharmacology , Receptors, GABA-B/drug effects , Retinal Ganglion Cells/drug effects , Taurine/pharmacology , Animals , Cell Survival/drug effects , Cells, Cultured , Male , Rats , Rats, Long-Evans , Receptors, GABA-B/metabolism , Retinal Ganglion Cells/metabolism
5.
Invest Ophthalmol Vis Sci ; 57(11): 4692-703, 2016 09 01.
Article in English | MEDLINE | ID: mdl-27607415

ABSTRACT

PURPOSE: Taurine depletion is known to induce photoreceptor degeneration and was recently found to also trigger retinal ganglion cell (RGC) loss similar to the retinal toxicity of vigabatrin. Our objective was to study the topographical loss of RGCs and cone photoreceptors, with a distinction between the two cone types (S- and L- cones) in an animal model of induced taurine depletion. METHODS: We used the taurine transporter (Tau-T) inhibitor, guanidoethane sulfonate (GES), to induce taurine depletion at a concentration of 1% in the drinking water. Spectral-domain optical coherence tomography (SD-OCT) and electroretinograms (ERG) were performed on animals after 2 months of GES treatment administered through the drinking water. Retinas were dissected as wholemounts and immunodetection of Brn3a (RGC), S-opsin (S-cones), and L-opsin (L-cones) was performed. The number of Brn3a+ RGCs, and L- and S-opsin+ cones was automatically quantified and their retinal distribution studied using isodensity maps. RESULTS: The treatment resulted in a significant reduction in plasma taurine levels and a profound dysfunction of visual performance as shown by ERG recordings. Optical coherence tomography analysis revealed that the retina was thinner in the taurine-depleted group. S-opsin+cones were more affected (36%) than L-opsin+cones (27%) with greater cone cell loss in the dorsal area whereas RGC loss (12%) was uniformly distributed. CONCLUSIONS: This study confirms that taurine depletion causes RGC and cone loss. Electroretinograms results show that taurine depletion induces retinal dysfunction in photoreceptors and in the inner retina. It establishes a gradient of cell loss depending on the cell type from S-opsin+cones, L-opsin+cones, to RGCs. The greater cell loss in the dorsal retina and of the S-cone population may underline different cellular mechanisms of cellular degeneration and suggests that S-cones may be more sensitive to light-induced retinal toxicity enhanced by the taurine depletion.


Subject(s)
Retinal Cone Photoreceptor Cells/pathology , Retinal Degeneration/diagnosis , Retinal Ganglion Cells/pathology , Taurine/metabolism , Animals , Cell Count , Disease Models, Animal , Electroretinography , Immunohistochemistry , Male , Mice , Mice, Inbred BALB C , Retinal Cone Photoreceptor Cells/metabolism , Retinal Degeneration/metabolism , Retinal Ganglion Cells/metabolism , Tomography, Optical Coherence
6.
Article in English | MEDLINE | ID: mdl-26476437

ABSTRACT

BACKGROUND: Most currently available active antidepressant drugs are selective serotonin/noradrenaline reuptake inhibitors. However, as their clinical efficacy is not immediate, long-term administration is often accompanied by substantial side effects, and numerous patients remain non- or partial responders. We have recently found that the synthetic neurosteroid derivative 3ß-methoxypregnenolone, which binds to the microtubule-associated protein-2, can provide a novel therapeutic approach in experimental model of depressive disorders in rats. To further validate the antidepressant-like efficacy of 3ß-methoxypregnenolone, we investigated effects of a longer treatment (4-week oral administration; 50mg/kg/d) in a nonrodent species, the tree shrew, exposed to psychosocial stress that elicits close-to-human alterations observed in patients with depressive disorders. METHODS: During the experimental period, physiological parameters were registered, including core body temperature and electroencephalogram, while animals were videotaped to analyze their avoidance behavior. Morning urine samples were collected for measurements of cortisol and noradrenaline levels. RESULTS: We found that treatment with 3ß-methoxypregnenolone abolished stress-triggered avoidance behavior and prevented hormone hypersecretion, hypothermia, and sleep disturbances, further suggesting its antidepressant-like efficacy. Comparative treatment with fluoxetine also prevented some of the physiological alterations, while the hypersecretion of cortisol and sleep disturbances were not or partially restored by fluoxetine, suggesting a better efficacy of 3ß-methoxypregnenolone. Alpha-tubulin isoforms were measured in hippocampi: we found that 3ß-methoxypregnenolone reversed the specific decrease in acetylation of α-tubulin induced by psychosocial stress, while it did not modify the psychosocial stress-elicited reduction of tyrosinated α-tubulin. CONCLUSIONS: Taken together, these data strongly suggest a potent antidepressant-like effect of 3ß-methoxypregnenolone on translational parameters.


Subject(s)
Antidepressive Agents/pharmacology , Pregnenolone/analogs & derivatives , Stress, Psychological/drug therapy , Stress, Psychological/physiopathology , Administration, Oral , Animals , Antidepressive Agents/blood , Avoidance Learning/drug effects , Avoidance Learning/physiology , Body Temperature/drug effects , Body Temperature/physiology , Disease Models, Animal , Drug Evaluation, Preclinical , Hippocampus/drug effects , Hippocampus/metabolism , Hydrocortisone/urine , Male , Motor Activity/drug effects , Motor Activity/physiology , Norepinephrine/urine , Pregnenolone/blood , Pregnenolone/pharmacology , Sleep/drug effects , Sleep/physiology , Social Behavior , Tubulin/metabolism , Tupaiidae
7.
PLoS One ; 9(11): e111873, 2014.
Article in English | MEDLINE | ID: mdl-25380252

ABSTRACT

In both humans and dogs, the primary risk factor for glaucoma is high intraocular pressure (IOP), which may be caused by iridocorneal angle (ICA) abnormalities. Oxidative stress has also been implicated in retinal ganglion cell damage associated with glaucoma. A suspected inherited form of glaucoma was recently identified in Eurasier dogs (EDs), a breed for which pedigrees are readily available. Because of difficulties in assessing ICA morphology in dogs with advanced glaucoma, we selected a cohort of apparently healthy dogsfor the investigation of ICA morphological status, IOP and plasma concentrations of oxidative stress biomarkers. We aimed to establish correlations between these factors, to identify predictive markers of glaucoma in this dog breed. A cohort of 28 subjects, volunteered for inclusion by their owners, was selected by veterinary surgeons. These dogs were assigned to four groups: young males, young females (1-3 years old), adult males and adult females (4-8 years old). Ocular examination included ophthalmoscopy, tonometry, gonioscopy, biometry and ultrasound biomicroscopy (UBM), and the evaluation of oxidative stress biomarkers consisting of measurements of plasma glutathione peroxidase (GP) activity and taurine and metabolic precursor (methionine and cysteine) concentrations in plasma. The prevalence of pectinate ligament abnormalities was significantly higher in adult EDs than in young dogs. Moreover, in adult females, high IOP was significantly correlated with a short axial globe length, and a particularly large distance between Schwalbe's line and the anterior lens capsule. GP activity levels were significantly lower in EDs than in a randomized control group of dogs, and plasma taurine concentrations were higher. Hence, ICA abnormalities were associated with weaker antioxidant defenses in EDs, potentially counteracted by higher plasma taurine concentrations. This study suggests that EDs may constitute an appropriate canine model for the development of glaucoma. This cohort will be used as a sentinel for longitudinal monitoring.


Subject(s)
Biometry , Glaucoma/diagnosis , Health , Animals , Cohort Studies , Disease Susceptibility , Dogs , Female , Glaucoma/diagnostic imaging , Glaucoma/metabolism , Glaucoma/physiopathology , Gonioscopy , Intraocular Pressure , Male , Manometry , Ultrasonography
8.
Prog Retin Eye Res ; 41: 44-63, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24721186

ABSTRACT

Taurine is the most abundant amino acid in the retina. In the 1970s, it was thought to be involved in retinal diseases with photoreceptor degeneration, because cats on a taurine-free diet presented photoreceptor loss. However, with the exception of its introduction into baby milk and parenteral nutrition, taurine has not yet been incorporated into any commercial treatment with the aim of slowing photoreceptor degeneration. Our recent discovery that taurine depletion is involved in the retinal toxicity of the antiepileptic drug vigabatrin has returned taurine to the limelight in the field of neuroprotection. However, although the retinal toxicity of vigabatrin principally involves a deleterious effect on photoreceptors, retinal ganglion cells (RGCs) are also affected. These findings led us to investigate the possible role of taurine depletion in retinal diseases with RGC degeneration, such as glaucoma and diabetic retinopathy. The major antioxidant properties of taurine may influence disease processes. In addition, the efficacy of taurine is dependent on its uptake into retinal cells, microvascular endothelial cells and the retinal pigment epithelium. Disturbances of retinal vascular perfusion in these retinal diseases may therefore affect the retinal uptake of taurine, resulting in local depletion. The low plasma taurine concentrations observed in diabetic patients may further enhance such local decreases in taurine concentration. We here review the evidence for a role of taurine in retinal ganglion cell survival and studies suggesting that this compound may be involved in the pathophysiology of glaucoma or diabetic retinopathy. Along with other antioxidant molecules, taurine should therefore be seriously reconsidered as a potential treatment for such retinal diseases.


Subject(s)
Retinal Degeneration/prevention & control , Taurine/physiology , Animals , Humans , Neuroprotective Agents/therapeutic use , Retinal Degeneration/physiopathology , Taurine/chemistry , Taurine/therapeutic use
9.
Adv Exp Med Biol ; 775: 69-83, 2013.
Article in English | MEDLINE | ID: mdl-23392925

ABSTRACT

Retinal ganglion cells (RGCs) are spiking neurons, which send visual information to the brain, through the optic nerve. RGC degeneration occurs in retinal diseases, either as a primary process or secondary to photoreceptor loss. Mechanisms involved in this neuronal degeneration are still unclear and no drugs directly targeting RGC neuroprotection are yet available. Here, we show that taurine is one factor involved in preserving the RGC survival. Indeed, a taurine depletion induced by the antiepileptic drug, vigabatrin, was incriminated in its retinal toxicity leading to the RGC loss. Similarly, we showed that RGC degeneration can be induced by pharmacologically blocking the taurine-transporter with the chronic administration of a selective inhibitor, which results in a decrease in the taurine levels both in the plasma and in the retinal tissue. Finally, we found that taurine can directly prevent RGC degeneration, occurring either in serum-deprived pure RGC cultures or in animal models presenting an RGC loss (glaucomatous rats and the P23H rats, a model for retinitis pigmentosa). These data suggest that the retinal taurine level is a crucial marker to prevent RGC damage in major retinal diseases.


Subject(s)
Neuroprotective Agents/pharmacology , Retinal Ganglion Cells/drug effects , Retinal Ganglion Cells/pathology , Taurine/pharmacology , Animals , Cell Survival/drug effects , Disease Models, Animal , Glaucoma/complications , Glaucoma/drug therapy , Glaucoma/pathology , Membrane Glycoproteins/antagonists & inhibitors , Membrane Glycoproteins/metabolism , Membrane Transport Proteins/metabolism , Neuroprotective Agents/therapeutic use , Rats , Retinitis Pigmentosa/complications , Retinitis Pigmentosa/drug therapy , Retinitis Pigmentosa/pathology , Taurine/analogs & derivatives , Taurine/therapeutic use , Time Factors , Vigabatrin/administration & dosage , Vigabatrin/pharmacology
10.
Adv Exp Med Biol ; 775: 145-54, 2013.
Article in English | MEDLINE | ID: mdl-23392931

ABSTRACT

In the 70s, the amino acid taurine was found essential for photoreceptor survival. Recently, we found that taurine depletion can also trigger retinal ganglion cell degeneration both in vitro and in vivo. Therefore, evaluation of taurine levels could be a crucial biomarker for different pathologies of retinal ganglion cells such as glaucoma. Because different breeds of dog can develop glaucoma, we performed taurine measurements on plasma and aqueous humour samples from pet dogs. Here, we exposed results from a pilot study on normal selected breed of pet dogs, without any ocular pathology. Samples were collected by veterinarians who belong to the Réseau Européen d'Ophtalmologie Vétérinaire et de Vision Animale. Following measurements by high-performance liquid chromatography (HPLC), the averaged taurine concentration was 162.3 µM in the plasma and 51.8 µM in the aqueous humour. No correlation was observed between these two taurine concentrations, which exhibited a ratio close to 3. Further studies will determine if these taurine concentrations are changed in glaucomatous dogs.


Subject(s)
Aqueous Humor/metabolism , Dogs/blood , Taurine/blood , Animals , Breeding , Cystine/blood , Diet , Female , Health , Male , Methionine/blood , Pilot Projects , Taurine/biosynthesis
11.
PLoS One ; 7(10): e42017, 2012.
Article in English | MEDLINE | ID: mdl-23115615

ABSTRACT

Retinal ganglion cell (RGC) degeneration occurs in numerous retinal diseases leading to blindness, either as a primary process like in glaucoma, or secondary to photoreceptor loss. However, no commercial drug is yet directly targeting RGCs for their neuroprotection. In the 70s, taurine, a small sulfonic acid provided by nutrition, was found to be essential for the survival of photoreceptors, but this dependence was not related to any retinal disease. More recently, taurine deprivation was incriminated in the retinal toxicity of an antiepileptic drug. We demonstrate here that taurine can improve RGC survival in culture or in different animal models of RGC degeneration. Taurine effect on RGC survival was assessed in vitro on primary pure RCG cultures under serum-deprivation conditions, and on NMDA-treated retinal explants from adult rats. In vivo, taurine was administered through the drinking water in two glaucomatous animal models (DBA/2J mice and rats with vein occlusion) and in a model of Retinitis pigmentosa with secondary RGC degeneration (P23H rats). After a 6-day incubation, 1 mM taurine significantly enhanced RGCs survival (+68%), whereas control RGCs were cultured in a taurine-free medium, containing all natural amino-acids. This effect was found to rely on taurine-uptake by RGCs. Furthermore taurine (1 mM) partly prevented NMDA-induced RGC excitotoxicity. Finally, taurine supplementation increased RGC densities both in DBA/2J mice, in rats with vein occlusion and in P23H rats by contrast to controls drinking taurine-free water. This study indicates that enriched taurine nutrition can directly promote RGC survival through RGC intracellular pathways. It provides evidence that taurine can positively interfere with retinal degenerative diseases.


Subject(s)
Neuroprotective Agents/pharmacology , Retinal Ganglion Cells/drug effects , Taurine/pharmacology , Animals , Cell Survival/drug effects , Cells, Cultured , Mice , Mice, Inbred DBA , N-Methylaspartate/pharmacology , Photoreceptor Cells, Vertebrate/metabolism , Retinal Ganglion Cells/cytology
12.
Amino Acids ; 43(5): 1979-93, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22476345

ABSTRACT

In 1970s, taurine deficiency was reported to induce photoreceptor degeneration in cats and rats. Recently, we found that taurine deficiency contributes to the retinal toxicity of vigabatrin, an antiepileptic drug. However, in this toxicity, retinal ganglion cells were degenerating in parallel to cone photoreceptors. The aim of this study was to re-assess a classic mouse model of taurine deficiency following a treatment with guanidoethane sulfonate (GES), a taurine transporter inhibitor to determine whether retinal ganglion cells are also affected. GES treatment induced a significant reduction in the taurine plasma levels and a lower weight increase. At the functional level, photopic electroretinograms were reduced indicating a dysfunction in the cone pathway. A change in the autofluorescence appearance of the eye fundus was explained on histological sections by an increased autofluorescence of the retinal pigment epithelium. Although the general morphology of the retina was not affected, cell damages were indicated by the general increase in glial fibrillary acidic protein expression. When cell quantification was achieved on retinal sections, the number of outer/inner segments of cone photoreceptors was reduced (20 %) as the number of retinal ganglion cells (19 %). An abnormal synaptic plasticity of rod bipolar cell dendrites was also observed in GES-treated mice. These results indicate that taurine deficiency can not only lead to photoreceptor degeneration but also to retinal ganglion cell loss. Cone photoreceptors and retinal ganglion cells appear as the most sensitive cells to taurine deficiency. These results may explain the recent therapeutic interest of taurine in retinal degenerative pathologies.


Subject(s)
Eye Proteins/genetics , Glial Fibrillary Acidic Protein/genetics , Retinal Cone Photoreceptor Cells/pathology , Retinal Degeneration/pathology , Retinal Ganglion Cells/pathology , Retinal Pigment Epithelium/pathology , Taurine/deficiency , Animals , Biological Transport/drug effects , Disease Models, Animal , Electroretinography , Eye Proteins/metabolism , Gene Expression/drug effects , Glial Fibrillary Acidic Protein/metabolism , Guanidines/pharmacology , Male , Mice , Mice, Inbred BALB C , Neuronal Plasticity/drug effects , Retinal Cone Photoreceptor Cells/drug effects , Retinal Cone Photoreceptor Cells/metabolism , Retinal Degeneration/chemically induced , Retinal Degeneration/metabolism , Retinal Ganglion Cells/drug effects , Retinal Ganglion Cells/metabolism , Retinal Pigment Epithelium/drug effects , Retinal Pigment Epithelium/metabolism , Taurine/antagonists & inhibitors
13.
J Neurochem ; 118(5): 826-40, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21294731

ABSTRACT

Inflammation contributes to neurodegeneration in post-ischemic brain, diabetes, and Alzheimer's disease. Participants in this inflammatory response include activation of microglia and astrocytes. We studied the role of microglia treated with amyloid-ß peptide (Aß) on hemichannel activity of astrocytes subjected to hypoxia in high glucose. Reoxygenation after 3 h hypoxia in high glucose induced transient astroglial permeabilization via Cx43 hemichannels and reduction in intercellular communication via Cx43 cell-cell channels. Both responses were greater and longer lasting in astrocytes previously exposed for 24 h to conditioned medium from Aß-treated microglia (CM-Aß). The effects of CM-Aß were mimicked by TNF-α and IL-1ß and were abrogated by neutralizing TNF-α with soluble receptor and IL-1ß with a receptor antagonist. Astrocytes under basal conditions protected neurons against hypoxia, but exposure to CM-Aß made them toxic to neurons subjected to a sub-lethal hypoxia/reoxygenation episode, revealing the additive nature of the insults. Astrocytes exposed to CM-Aß induced permeabilization of cortical neurons through activation of neuronal pannexin 1 (Panx1) hemichannels by ATP and glutamate released through astroglial Cx43 hemichannels. In agreement, inhibition of NMDA or P2X receptors only partially reduced the activation of neuronal Panx1 hemichannels and neuronal mortality, but simultaneous inhibition of both receptors completely prevented the neurotoxic response. Therefore, we suggest that responses to ATP and glutamate converge in activation of neuronal Panx1 hemichannels. Thus, we propose that blocking hemichannels expressed by astrocytes and/or neurons in the inflamed nervous system could represent a novel and alternative strategy to reduce neuronal loss in various pathological states including Alzheimer's disease, diabetes and ischemia.


Subject(s)
Adenosine Triphosphate/metabolism , Astrocytes/metabolism , Connexin 43/metabolism , Connexins/metabolism , Glutamic Acid/metabolism , Nerve Tissue Proteins/metabolism , Neurons/physiology , Adenosine Triphosphate/pharmacology , Amyloid beta-Peptides/pharmacology , Animals , Animals, Newborn , Astrocytes/chemistry , Astrocytes/drug effects , Biotinylation/methods , Cell Death/drug effects , Cell Hypoxia/drug effects , Cell Hypoxia/physiology , Cells, Cultured , Cerebral Cortex/cytology , Coculture Techniques/methods , Connexin 43/deficiency , Connexins/pharmacology , Culture Media, Conditioned/pharmacology , Female , Fluoresceins , Glutamic Acid/pharmacology , Interleukin-1beta/metabolism , Lanthanum/pharmacology , Mice , Mice, Knockout , Microtubule-Associated Proteins/pharmacology , Nerve Tissue Proteins/pharmacology , Neurons/drug effects , Neuropeptides/pharmacology , Oxygen/pharmacology , Peptide Fragments/pharmacology , Time Factors , Tumor Necrosis Factor-alpha/metabolism
14.
Mol Cell Neurosci ; 45(1): 37-46, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20684043

ABSTRACT

Astrocytes express high levels of connexin43, a protein that forms two types of channels: gap junction channels for direct intercellular communication, and hemichannels for exchanges with the extracellular space. Inflammation induces connexin43 hemichannel activation, which has been proposed to be involved in neuroglial interactions. Here, we investigated the contribution of connexin43 to NMDA-induced excitotoxicity in neuron/astrocyte co-cultures, after treatment with a pro-inflammatory cytokine mixture, containing TNF-alpha and IL1-beta (Mix), that stimulated astroglial connexin43 hemichannel activity. Interestingly, NMDA treatment induced a higher amount of neurotoxicity in Mix-treated co-cultures than in untreated ones, whereas this extent of neurotoxicity was absent in enriched neuron cultures or in co-cultures with connexin43 knock-out astrocytes. Furthermore, application of connexin43 hemichannel blockers or a synthetic cannabinoid prevented the Mix-induced potentiated NMDA neurotoxicity. Altogether, these data demonstrate that inflammation-induced astroglial hemichannel activation plays a critical role in neuronal death and suggest a neuroprotective role of connexin43 hemichannel blockade.


Subject(s)
Astrocytes/metabolism , Connexin 43/metabolism , Cytokines/metabolism , Gap Junctions/metabolism , Animals , Astrocytes/cytology , Astrocytes/drug effects , Cell Death/physiology , Cells, Cultured , Coculture Techniques , Female , Fluorescent Dyes/metabolism , Interleukin-1beta/pharmacology , Mice , Mice, Knockout , N-Methylaspartate/metabolism , Neurons/cytology , Neurons/drug effects , Neurons/metabolism , Pregnancy , Tumor Necrosis Factor-alpha/pharmacology
15.
J Neurochem ; 111(6): 1383-97, 2009 Dec.
Article in English | MEDLINE | ID: mdl-20050288

ABSTRACT

Brain injuries as well as neurodegenerative diseases, are associated with neuro-inflammation characterized by astroglial and microglial activation and/or proliferation. Recently, we reported that lipopolysaccharide (LPS)-activation of microglia inhibits junctional channels and promotes hemichannels, two connexin43 functions in astrocytes. This opposite regulation is mediated by two pro-inflammatory cytokines, interleukin-1 beta and tumor necrosis factor-alpha, released from activated microglia. Because cannabinoids (CBs) have anti-inflammatory properties and their receptors are expressed by glial cells, we investigated on primary cortical cultures the effects of CB agonists, methanandamide and synthetic CBs on (i) cytokines released from LPS-activated microglia and (ii) connexin43 functions in astrocytes subjected to pro-inflammatory treatments. We observed that CBs inhibited the LPS-induced release of interleukin-1 beta and tumor necrosis factor-alpha from microglia. Moreover, the connexin43 dual regulation evoked by the pro-inflammatory treatments, was prevented by CB treatments. Pharmacological characterizations of CB actions on astrocytic connexin43 channels revealed that these effects were mainly mediated through CB1 receptors activation, although non-CB1/CB2 receptors seemed to mediate the action of the methanandamide. Altogether these data demonstrate that in inflammatory situations CBs exert, through the activation of different sub-types of glial CB receptors, a regulation on two functions of connexin43 channels in astrocytes known to be involved in neuron survival.


Subject(s)
Astrocytes/drug effects , Cannabinoids/metabolism , Connexin 43/metabolism , Connexins/metabolism , Lipopolysaccharides/pharmacology , Analgesics/pharmacology , Animals , Animals, Newborn , Arachidonic Acids/pharmacology , Astrocytes/metabolism , Benzoxazines/pharmacology , Cannabinoids/antagonists & inhibitors , Cannabinoids/pharmacology , Cells, Cultured , Cerebral Cortex/cytology , Culture Media, Conditioned/pharmacology , Cyclohexanols/pharmacology , Drug Interactions , Enzyme-Linked Immunosorbent Assay/methods , Ethidium/metabolism , Gene Expression Regulation/drug effects , Interleukin-1beta/metabolism , Maximum Tolerated Dose , Mice , Microglia/chemistry , Morpholines/pharmacology , Naphthalenes/pharmacology , Piperidines/pharmacology , Pyrazoles/pharmacology , Rimonabant , Tumor Necrosis Factor-alpha/metabolism
16.
J Neurosci ; 27(50): 13781-92, 2007 Dec 12.
Article in English | MEDLINE | ID: mdl-18077690

ABSTRACT

Astrocytes have a role in maintaining normal neuronal functions, some of which depend on connexins, protein subunits of gap junction channels and hemichannels. Under inflammatory conditions, microglia release cytokines, including interleukin-1beta and tumor necrosis factor-alpha, that reduce intercellular communication via gap junctions. Now, we demonstrate that either conditioned medium harvested from activated microglia or a mixture of these two cytokines enhances the cellular exchange with the extracellular milieu via Cx43 hemichannels. These changes in membrane permeability were not detected in astrocytes cultured from Cx43 knock-out mice and were abrogated by connexin hemichannel blockers, including La3+, mimetic peptides, and niflumic acid. Both the reduction in gap junctional communication and the increase in membrane permeability were mediated by a p38 mitogen-activated protein kinase-dependent pathway. However, the increase in membrane permeability, but not the gap junction inhibition, was rapidly reversed by the sulfhydryl reducing agent dithiothreitol, indicating that final regulatory mechanisms are different. Treatment with proinflammatory cytokines reduced the total and cell surface Cx43 levels, suggesting that the increase in membrane permeability was attributable to an increase in hemichannels activity. Indeed, unitary events of approximately 220 pS corresponding to Cx43 hemichannels were much more frequent in astrocytes treated with microglia conditioned medium than under control conditions. Finally, the effect of cytokines enhanced the uptake and reduced the intercellular diffusion of glucose, which might explain changes in the metabolic status of astrocytes under inflammatory conditions. Accordingly, this opposite regulation may affect glucose trafficking and certainly will modify the metabolic status of astrocytes involved in brain inflammation.


Subject(s)
Astrocytes/metabolism , Connexin 43/metabolism , Cytokines/physiology , Gap Junctions/metabolism , Inflammation Mediators/metabolism , Microglia/metabolism , Animals , Animals, Newborn , Astrocytes/cytology , Astrocytes/drug effects , Biological Transport/drug effects , Cell Communication/drug effects , Cell Membrane Permeability/drug effects , Cells, Cultured , Connexin 43/drug effects , Connexin 43/genetics , Culture Media, Conditioned/pharmacology , Cytokines/pharmacology , Gap Junctions/drug effects , Glucose/pharmacokinetics , Inflammation Mediators/pharmacology , Lipopolysaccharides/pharmacology , Mice , Mice, Knockout , Microglia/cytology , Microglia/drug effects , Patch-Clamp Techniques , p38 Mitogen-Activated Protein Kinases/metabolism
17.
FASEB J ; 20(3): 494-6, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16423877

ABSTRACT

Brain inflammation is characterized by a reactive gliosis involving the activation of astrocytes and microglia. This process, common to many brain injuries and diseases, underlies important phenotypic changes in these two glial cell types. One characteristic feature of astrocytes is their high level of intercellular communication mediated by gap junctions. Previously, we have reported that astrocyte gap junctional communication (AGJC) and the expression of connexin 43 (Cx43), the main constitutive protein of gap junctions, are inhibited in microglia (MG)-astrocyte cocultures. Here, we report that bacterial lipopolysaccharide activation of microglia increases their inhibitory effect on Cx43 expression and AGJC. This inhibition is mimicked by treating astrocyte cultures with conditioned medium harvested from activated microglia. Interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) were identified as being the main factors responsible for this conditioned medium-mediated activity. Interestingly, an inflammatory response characterized by MG activation and reactive astrocytes occurs in Alzheimer's disease, at sites of beta-amyloid (Abeta) deposits. We found that this peptide potentiates the inhibitory effect of a conditioned medium diluted at a concentration that is not effective per se. This potentiation is prevented by treating astrocytes with specific blockers of IL-1beta and TNF-alpha activities. Thus, the suppression of communication between astrocytes, induced by activated MG could contribute to the proposed role of reactive gliosis in this neurodegenerative disease.


Subject(s)
Amyloid beta-Peptides/pharmacology , Astrocytes/drug effects , Gap Junctions/drug effects , Interleukin-1/pharmacology , Microglia/metabolism , Peptide Fragments/pharmacology , Tumor Necrosis Factor-alpha/pharmacology , Animals , Astrocytes/physiology , Cell Communication/drug effects , Cells, Cultured/drug effects , Cells, Cultured/physiology , Connexin 43/biosynthesis , Culture Media, Conditioned/pharmacology , Gap Junctions/physiology , Interleukin 1 Receptor Antagonist Protein , Interleukin-1/antagonists & inhibitors , Interleukin-1/metabolism , Lipopolysaccharides/pharmacology , Mice , Nerve Degeneration , Sialoglycoproteins/pharmacology , Tumor Necrosis Factor-alpha/antagonists & inhibitors , Tumor Necrosis Factor-alpha/metabolism
18.
J Neurochem ; 95(6): 1713-23, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16219031

ABSTRACT

Antagonists at NK1 substance P receptors have demonstrated similar antidepressant properties in both animal paradigms and in human as selective serotonin reuptake inhibitors (SSRIs) that induce desensitization of 5-HT 1A autoreceptors within the dorsal raphe nucleus (DRN). We investigated whether this receptor adaptation also occurs upon NK1 receptor blockade. C57B/L6J mice were treated for 21 days with the selective NK1 receptor antagonist GR 205171 (10 mg/kg daily) through subcutaneously implanted osmotic mini pumps, and DRN 5-HT 1A autoreceptor functioning was assessed using various approaches. Recording of DRN serotonergic neurons in brainstem slices showed that GR 205171 treatment reduced (by approximately 1.5 fold) the potency of the 5-HT 1A receptor agonist, ipsapirone, to inhibit cell firing. In parallel, the 5-HT 1A autoreceptor-mediated [35S]GTP-gamma-S binding induced by 5-carboxamidotryptamine onto the DRN in brainstem sections was significantly decreased in GR 205171-treated mice. In vivo microdialysis showed that the cortical 5-HT overflow caused by acute injection of the SSRI paroxetine (1 mg/kg) was twice as high in GR 205171-treated as in vehicle-treated controls. In the DRN, basal 5-HT outflow was significantly enhanced by GR 205171 treatment. These data supported the hypothesis that chronic NK1 receptor blockade induces a functional desensitization of 5-HT 1A autoreceptors similar to that observed with SSRIs.


Subject(s)
Autoreceptors/drug effects , Neurokinin-1 Receptor Antagonists , Raphe Nuclei/drug effects , Receptor, Serotonin, 5-HT1A/drug effects , Serotonin Antagonists , Animals , Autoradiography , Electrophysiology , Guanosine 5'-O-(3-Thiotriphosphate)/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Microdialysis , Paroxetine/pharmacology , Piperidines/pharmacology , Receptors, Neurokinin-1/genetics , Selective Serotonin Reuptake Inhibitors/pharmacology , Tetrazoles/pharmacology
19.
Eur J Pharmacol ; 492(1): 41-8, 2004 May 10.
Article in English | MEDLINE | ID: mdl-15145704

ABSTRACT

We recently demonstrated that mice lacking the gene for substance P (neurokinin 1) receptors (NK1-/-) show improved cortical dialysate serotonin (5-HT) responses to paroxetine [J. Neurosci. 21 (2001) 8188]. To test for changes that may involve the 5-HT transporter (5-HTT) in these mutant mice, in vivo/in vitro studies were performed. Autoradiographic quantification of 5-HTT was performed: [3H]citalopram binding did not reveal any modification of 5-HT binding sites in the dorsal raphe nucleus (DRN) of wild-type NK1+/+ control and mutant NK1-/- mice. These results were further confirmed by 5-HTT mRNA quantification using competitive reverse transcription and polymerase chain reaction (RT-PCR) assay, which showed similar messenger levels in the DRN of both mice genotypes. The functional status of 5-HTT in vivo was tested by using the zero net flux method of quantitative microdialysis in two serotonergic nerve terminal regions, the frontal cortex and ventral hippocampus, of wild-type NK1+/+ and NK1-/- mice. Neither basal extracellular 5-HT levels nor the 5-HT extraction fraction of the probe (Ed an index of 5-HT uptake in vivo) differed between wild-type and mutant mice in the two brain regions studied. These results suggest that no compensatory response to the constitutive deletion of the tachykinin NK1 receptor involving changes in the activity of the selective 5-HT transporter occurred in the DRN, frontal cortex and ventral hippocampus in mice.


Subject(s)
Membrane Glycoproteins/metabolism , Membrane Transport Proteins/metabolism , Nerve Tissue Proteins/metabolism , Raphe Nuclei/metabolism , Receptors, Neurokinin-1/genetics , Serotonin/metabolism , Animals , Autoradiography , Citalopram/pharmacology , Extracellular Fluid/metabolism , Frontal Lobe/drug effects , Frontal Lobe/metabolism , Hippocampus/drug effects , Hippocampus/metabolism , Membrane Glycoproteins/genetics , Membrane Transport Proteins/genetics , Mice , Mice, Knockout , Microdialysis , Nerve Tissue Proteins/genetics , RNA, Messenger/analysis , RNA, Messenger/metabolism , Raphe Nuclei/drug effects , Reverse Transcriptase Polymerase Chain Reaction , Serotonin/analysis , Serotonin Plasma Membrane Transport Proteins , Selective Serotonin Reuptake Inhibitors/pharmacology
20.
J Neurochem ; 89(1): 54-63, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15030389

ABSTRACT

Abstract Substance P antagonists of the neurokinin-1 receptor type (NK1) are gaining growing interest as new antidepressant therapies. It has been postulated that these drugs exert this putative therapeutic effect without direct interactions with serotonin (5-HT) neurones. Our recent microdialysis experiment performed in NK1 receptor knockout mice suggested evidence of changes in 5-HT neuronal function (Froger et al. 2001). The aim of the present study was to evaluate the effects of coadministration of the selective 5-HT reuptake inhibitor (SSRI) paroxetine with a NK1 receptor antagonist (GR205171 or L733060), given either intraperitoneally (i.p.) or locally into the dorsal raphe nucleus, on extracellular levels of 5-HT ([5-HT]ext) in the frontal cortex and the dorsal raphe nucleus using in vivo microdialysis in awake, freely moving mice. The systemic or intraraphe administration of a NK1 receptor antagonist did not change basal cortical [5-HT]ext in mice. A single systemic dose of paroxetine (4 mg/kg; i.p.) resulted in a statistically significant increase in [5-HT]ext with a larger extent in the dorsal raphe nucleus (+ 138% over basal AUC values), than in the frontal cortex (+ 52% over basal AUC values). Co-administration of paroxetine (4 mg/kg; i.p.) with the NK1 receptor antagonists, GR205171 (30 mg/kg; i.p.) or L733060 (40 mg/kg; i.p.), potentiated the effects of paroxetine on cortical [5-HT]ext in wild-type mice, whereas GR205171 (30 mg/kg; i.p.) had no effect on paroxetine-induced increase in cortical [5-HT]ext in NK1 receptor knock-out mice. When GR205171 (300 micro mol/L) was perfused by 'reverse microdialysis' into the dorsal raphe nucleus, it potentiated the effects of paroxetine on cortical [5-HT]ext, and inhibited paroxetine-induced increase in [5-HT]ext in the dorsal raphe nucleus. Finally, in mice whose 5-HT transporters were first blocked by a local perfusion of 1 micro mol/L of citalopram into the frontal cortex, a single dose of paroxetine (4 mg/kg i.p.) decreased cortical 5-HT release, and GR205171 (30 mg/kg i.p.) reversed this effect. The present findings suggest that NK1 receptor antagonists, when combined with a SSRI, augment 5-HT release by modulating substance P/5-HT interactions in the dorsal raphe nucleus.


Subject(s)
Frontal Lobe/metabolism , Neurokinin-1 Receptor Antagonists , Piperidines/pharmacology , Raphe Nuclei/metabolism , Selective Serotonin Reuptake Inhibitors/pharmacology , Serotonin/metabolism , Tetrazoles/pharmacology , Animals , Drug Administration Routes , Extracellular Fluid/chemistry , Extracellular Fluid/metabolism , Frontal Lobe/drug effects , Injections, Intraperitoneal , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Microdialysis , Paroxetine/pharmacology , Perfusion , Raphe Nuclei/drug effects , Receptors, Neurokinin-1/metabolism , Serotonin/analysis
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