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2.
Anal Bioanal Chem ; 398(6): 2723-30, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20865406

ABSTRACT

A new method based on hydrophilic interaction chromatography-electrospray ionisation-tandem mass spectrometry (HILIC-ESI-MS/MS) coupled to the use of a stable isotope labelled substrate was developed to study the metabolism of choline (Cho) compounds in two human glioblastoma multiform (GBM) cell lines with different responses to ionising radiation. Analysis was performed in the positive ion mode using multiple reaction monitoring. This fast, sensitive and selective method enabled the profiling of both hydrophilic and lipophilic Cho-containing compounds, to analyse specifically different phosphatidylcholine (PtdCho) molecular species, and to measure simultaneously native and labelled Cho metabolites. Radioresistant (SF763) and radiosensitive (SF767) cells were incubated for 8 h with d(9)-Cho. Higher native Cho and phosphocholine (PCho) concentrations and higher uptake of d(9)-Cho and formation of d(9)-PCho were found in the radioresistant cell line. The similar low concentrations of native cytidine 5'-diphosphocholine (CDP-Cho) and d(9)-CDP-Cho in both cell lines show that CDP-Cho is the limiting metabolite in the two models. The turnovers (percentage of each d(9)-Cho compound in its respective pool, i.e. native + labelled) were lower in radioresistant cells for all Cho compounds, suggesting a global PtdCho metabolism more active in radiosensitive cells that could be related to their higher proliferation rate.


Subject(s)
Brain Neoplasms/radiotherapy , Choline/analysis , Chromatography, Liquid/methods , Glioblastoma/radiotherapy , Spectrometry, Mass, Electrospray Ionization/methods , Tandem Mass Spectrometry/methods , Brain Neoplasms/metabolism , Cell Culture Techniques , Cell Line, Tumor/metabolism , Cell Line, Tumor/radiation effects , Choline/metabolism , Chromatography, Liquid/instrumentation , Glioblastoma/metabolism , Humans , Hydrophobic and Hydrophilic Interactions , Spectrometry, Mass, Electrospray Ionization/instrumentation , Tandem Mass Spectrometry/instrumentation
3.
Presse Med ; 34(22 Pt 1): 1715-6, 2005 Dec 17.
Article in French | MEDLINE | ID: mdl-16374393

ABSTRACT

INTRODUCTION: Toxocariasis is a common helminth infection that causes visceral larva migrans in humans. It has a wide and varied clinical spectrum. CASE: A 60-year-old woman consulted after 6 months of recurrent biliary pain with fever. Laboratory tests showed moderate disturbance of liver enzymes and hypereosinophilia (10 400/mm3). Imaging studies revealed a pseudotumor in hepatic segment IV, hyperechoic in abdominal ultrasound and low density in computed tomography. The diagnostic conclusion after ultrasound-guided liver biopsy was unspecific granulomatous hepatitis and eosinophilic infiltrate. The course of the antibody titer to Toxocara canis finally resulted in a diagnosis of Toxocara infection. Outcome was good following treatment with diethylcarbamazine. DISCUSSION: Some clinical features of liver toxocariasis can mimic tumors and may be interpreted histologically as granulomatous hepatitis, eosinophilic infiltrate of the hepatic portal vein, and/or necrotizing eosinophilic abscesses. Treatment is not yet well codified, but the symptomatic forms call for albendazole or diethylcarbamazine.


Subject(s)
Toxocariasis/diagnosis , Diagnosis, Differential , Diethylcarbamazine/therapeutic use , Eosinophilia/parasitology , Female , Filaricides/therapeutic use , Humans , Liver Neoplasms/diagnosis , Middle Aged , Toxocariasis/drug therapy
5.
J Cancer Res Clin Oncol ; 127(6): 368-74, 2001.
Article in English | MEDLINE | ID: mdl-11414197

ABSTRACT

PURPOSE: The glutathione detoxification pathway includes glutathione S-transferase (GST) and peroxidase (GPX) isoenzymes as well as glutathione reductase (GSSR). Though well established from cultured cancer cell lines, its involvement in resistance is still unclear in the tumours. This study aimed to describe the parameters that influence the glutathione contents and associated activities in breast cancer. METHODS: The components of the glutathione pathway were measured in the tumours from 41 women with primary breast cancer in comparison with those in the matched tumour-free samples. Appropriate statistical studies (regression analysis, Wilcoxon signed rank test) explored the influence of clinical and prognostic factors. RESULTS: Reduced and total glutathione contents were largely increased (P < 0.0001) and all related activities were significantly enhanced in the tumours. Interindividual variations were described, probably due to various parameters (age, menopause, axillary lymph node status, S and G2 + M cell fractions, ER, cathepsin-D and c-ErbB-2 expressions) that influence particular components of the glutathione pathway, especially the glutathione levels. CONCLUSIONS: The breast tumours improved their redox status and detoxification capacities depending on various parameters of significance for cell proliferation and aggressiveness, which supports the involvement of the glutathione pathway in malignant cell resistance to oxidative stress and apoptosis.


Subject(s)
Breast Neoplasms/enzymology , Glutathione/metabolism , Adult , Aged , Breast Neoplasms/diagnosis , Breast Neoplasms/pathology , Cathepsin D/analysis , Drug Resistance, Neoplasm , Female , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Glutathione Transferase/metabolism , Humans , Inactivation, Metabolic , Menopause , Middle Aged , Receptor, ErbB-2/analysis , Receptors, Estrogen/analysis
6.
J Ethnopharmacol ; 74(3): 205-15, 2001 Mar 03.
Article in English | MEDLINE | ID: mdl-11274819

ABSTRACT

Previous studies realized in the laboratory have indicated that application of experimental stress (such as unavoidable footshock) induced significant behavioral, gastric and immunological alterations in mice. The aim of this study was to evaluate effects of low doses of Atropa belladonna L., Gelsemium sempervirens L. and Poumon histamine on stress-induced behavioral, immunological and gastric alterations. Locomotor, postural and exploratory activities have been evaluated by two behavioral tests: light/dark box and staircase tests. Immunological studies were investigated to count white blood cells subpopulations (lymphocytes, neutrophils, monocytes and basophils) by coulter counter. The severity of gastric erosions was evaluated by microscopic technique in mice after experimental stress. The results have demonstrated that low doses of G. sempervirens L. and A. belladonna L. had a significant neurotropic and protective effects on behavioral and gastric alterations induced by experimental stress. The immunological protective effects observed were probably induced via their neurotropic effects. The P. histamine showed a significant immunoprotective and gastroprotective effect in mice exposed to experimental stress.


Subject(s)
Alkaloids/pharmacology , Behavior, Animal/drug effects , Belladonna Alkaloids/pharmacology , Gastric Mucosa/drug effects , Plant Extracts/pharmacology , Stress, Psychological/drug therapy , Stress, Psychological/immunology , Alkaloids/immunology , Analysis of Variance , Animals , Belladonna Alkaloids/immunology , Lymphocyte Count , Male , Mice , Stress, Psychological/etiology
7.
Clin Lab Haematol ; 22(5): 297-301, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11122273

ABSTRACT

The history of a 28-year-old woman with anaplastic large cell lymphoma (ALCL) in the first trimester of her pregnancy is reported. Investigations allowed to diagnose a T-cell CD30 positive ALCL, which appearance is rare during pregnancy. Moreover, the atypical lymphoid cells were found in the peripheral blood and were predominantly small to medium sized with nuclear irregularities and cytoplasmic azurophilic granules, which allowed the hypothesis of leukaemic presentation of a small cell variant ALCL. A variant of the t(2:5)(p23:q35) was found [del(2)(p22)]. The patient died shortly after diagnosis.


Subject(s)
Lymphoma, Large-Cell, Anaplastic/pathology , Pregnancy Complications, Neoplastic/pathology , Adult , Chromosome Deletion , Chromosomes, Human, Pair 2/genetics , Chromosomes, Human, Pair 5/genetics , Fatal Outcome , Female , Humans , Lymphocytes/pathology , Lymphoma, Large-Cell, Anaplastic/genetics , Pregnancy , Translocation, Genetic
8.
Cancer Lett ; 158(1): 7-16, 2000 Sep 29.
Article in English | MEDLINE | ID: mdl-10940503

ABSTRACT

Glutathione and the associated enzymes, glutathione S-transferases, peroxidases, and reductase, have been implicated in cancer chemoresistance. This pathway was investigated in paired cancerous and peritumoral breast samples from 41 women. The tumours exhibited a higher redox status as deduced from increased transferase, peroxidase, and reductase activities and from higher total and reduced glutathione contents. Several components were strongly correlated in peritumoral tissues, suggesting a highly co-ordinated glutathione pathway that appeared disrupted in breast tumours with only a few correlations left. Therefore, resistance could spontaneously result from deregulated variations in the glutathione pathway, which might be relevant to the malignant disease progression.


Subject(s)
Breast Neoplasms/metabolism , Breast/metabolism , Glutathione/metabolism , Inactivation, Metabolic/physiology , Adult , Aged , Aged, 80 and over , Breast/enzymology , Breast Neoplasms/enzymology , Female , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Glutathione Transferase/metabolism , Humans , Middle Aged
10.
Gastroenterol Clin Biol ; 13(5): 505-7, 1989 May.
Article in English | MEDLINE | ID: mdl-2753287

ABSTRACT

A round mass was found by an abdominal ultrasonography in the head of the pancreas of a 63 year-old woman. Ultrasonography was performed because of abdominal pain. Percutaneous aspiration cytology suggested pancreatic adenocarcinoma. A pancreatoduodenectomy was performed heading to the discovery of an intrapancreatic lipoma. This lesion should be added to the list of benign connective tissue tumors of the pancreas.


Subject(s)
Lipoma/pathology , Pancreatic Neoplasms/pathology , Adenocarcinoma/pathology , Biopsy, Needle , Diagnosis, Differential , Female , Humans , Lipoma/epidemiology , Middle Aged , Pancreatic Neoplasms/epidemiology
11.
J Chir (Paris) ; 122(2): 87-91, 1985 Feb.
Article in French | MEDLINE | ID: mdl-3997985

ABSTRACT

2 cases of rectal metastatic breast carcinoma were treated by an abdomino-perineal resection. The rectal involvement is quite uncommon, and only 6 others cases treated with surgery have been described in the international literature. We think that only radical surgery can give a good palliation.


Subject(s)
Adenocarcinoma , Breast Neoplasms , Rectal Neoplasms/secondary , Aged , Amputation, Surgical/methods , Female , Follow-Up Studies , Humans , Middle Aged , Prognosis , Rectal Neoplasms/pathology , Rectal Neoplasms/surgery
14.
Bull Assoc Anat (Nancy) ; 67(198): 325-9, 1983 Sep.
Article in French | MEDLINE | ID: mdl-6675745

ABSTRACT

The authors study the Pancreas divisum's rate in 242 cadavers. With two successive studies, they establish a rate of about 6.6%. They conform the sexual disparity and the presence of muscular fibre cells on a level with papilla duodeni minor.


Subject(s)
Pancreas/abnormalities , Female , Humans , Male , Pancreas/embryology , Pancreas/pathology , Pancreatic Ducts/pathology
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