[Fatal hydrogen sulphide (H2S) poisoning in "confined spaces"]. / Le intossicazioni professionali mortali da Idrogeno Solforato (H2S) in "ambienti cofinati".

This paper deals with the problem of fatal accidents that occur repeatedly in "confined spaces", with particular attention to exposure to hydrogen sulfide (H2S). This, at high concentrations, appears to be the most compatible with the dynamics of many recent incidents with sudden death. H2S offers little chance to escape at high concentrations because of the smell that causes paralysis of olfactory nerves and sudden loss of consciousness. Besides the problem of acute exposure to high concentrations, health effects may also be caused by prolonged and repeated exposures to lower doses: at low concentrations can occur eyes irritation with keratoconjunctivitis and, at higher concentrations, the risk of pulmonary oedema or chronic central nervous system sequelae. In this paper several aspects are detailed, including an interpretative analysis of the content of Articles 66 and 121 of Legislative Decree 81/2008 and subsequent amendments, the work contexts where H2S is present as a raw material or product of the process and the effects on human health. Moreover, due to few epidemiological initiatives at national level, some aspects related to the accident dynamics are taken into account through the reconstruction of cases of fatal accidents occurred in Italy in recent years and comparing it with that reported in the literature of other Countries.

[Presence of the microbiological risk in Umbrian sawmills]. / Presenza del rischio microbiologico nel settore delle falegnamerie umbre.

The purpose of this study was to quantify and identify the airborne microbial contamination in Umbria Sawmills. In this paper we reported the preliminary results of our analysis. Microbial contaminants (fungi and bacteria) were assessed with passive (IMA Standard) and active (SAS microbial sampler) methods. There were significant differences of bacterial and/or fungal CFU/m3 values between the outdoor and indoor environments during the normal sawmills activity. Staphylococcus, Sphingomonas, Pasteurella, were the most predominant bacteria. The most predominant isolated fungi belong to Cladosporium, Penicillium, Alternaria and Aspergillus genus.

Phylogenetic analysis of Phlebotomus species belonging to the subgenus Larroussius (Diptera, psychodidae) by ITS2 rDNA sequences.

In the genealogy of Phlebotomus (Diptera: Psychodidae), morphological analyses have indicated that the subgenus Larroussius is a monophyletic group which is most closely related to the subgenera Transphlebotomus and Adlerius. We conducted a phylogenetic analysis of the relationships among six representative species of the subgenus Larroussius and one species representatitive of the Phlebotomus subgenus, assessing sequences of the Second Internal Transcribed Spacer (ITS2) of the ribosomal RNA (rRNA). Three of the species (P. perniciosus, P. ariasi and P. perfiliewi perfiliewi) were collected in different parts of the Mediterranean area. The trees estimated from parsimony and neighbour-joining analyses supported the monophyly of the Larroussius subgenus inferred from the morphological analysis. According to our data, P. ariasi may be a sister group to the rest of the Larroussius subgenus, although additional sequence data are needed to confirm this observation. Our results suggest that P. perniciosus and P. longicuspis are distinct species, in spite of the fact that there are only slight morphological differences. The strict congruence between the phylogeny of the Larroussius subgenus inferred from the ITS2 sequences and that based on morphological studies further confirmed the ability of the spacer sequence to identify recently-derived affiliations.

Rate of infection of Ixodes ricinus ticks with Borrelia burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii and group VS116 in an endemic focus of Lyme disease in Italy.

A study to evaluate the natural rate of infection of Ixodes ricinus with Borrelia burgdorferi sensu lato was carried out in an endemic focus of Lyme disease in the Trieste area in northern Italy. Two-hundred and twenty-seven ticks collected in ten different stations were tested individually for the presence of the spirochetes using polymerase chain reaction techniques able to identify both Borrelia burgdorferi sensu lato and the four genospecies (Borrelia burgdorferi sensu stricto, Borrelia garinii. Borrelia afzelii and group VS116). Multiple infection of individual ticks was found. The infection rate ranged from 0-70%. Infection of Ixodes ricinus with Borrelia burgdorferi group VS116 was found for the first time in Italy in both a high and a low endemic focus of Lyme disease.

Distribution and density of house dust mites Dermatophagoides spp. (Acarina:Pyroglypidae) in the mattresses of two areas of Rome, Italy.

The distribution of Dermatophagoides farinae and D. pteronyssinus in two groups of houses in Rome was investigated by means of weekly sampling of dust on mattresses during the period of high mite density (May-July, 1994). All mattresses were infested; overall 4,179 live and dead mites were collected. In the two examined areas, a difference in species distribution was observed. D farinae was always the prevalent species, it was 94.7% in area A and 64.1% in area B; only in houses 9 and 10 D. pteronyssinus was the most abundant species (71.4% and 45.8%). Housekeeping practices were investigated by interviewing the tenants and relative humidily and indoor/outdoor temperature were measured during each weekly sampling. A correlation analysis revealed a significant relationship between housekeeping practices and mite density (r = 0.83). The poorest housekeeping practices produced the heaviest mite infestation, while good ones were associated with low densities.

[Distribution of dog ticks (Rhipicephalus sanguineus Latreille, 1806) in Italy: a public health problem]. / Diffusione della zecca del cane (Rhipicephalus sanguineus Latreille, 1806) in Italia: un problema di salute pubblica.

The dog tick, Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae), is a public health problem since it is widely distributed in Italy and because of its role in the transmission of many pathogens to man and dog which is its preferred host. This review deals with the main aspects of the biology and ecology of Rh. sanguineus habitat, developmental time, distribution and seasonality, behaviour and vector competence. Moreover, the preminent role of Rh, sanguineus in the transmission of boutonneuse fever is discussed.

[Temporal distribution of ticks (Acarina: Ixodidae) in "Macchia Grande" park in Manziana in the province of Rome]. / Distribuzione temporale di zecche (Acarina: Ixodidae) nel parco di "Macchia Grande" di Manziana in provincia di Roma.

Temporal distribution of tick species was studied in the woodland "Macchia Grande" of Manziana in the province of Rome, Italy. A total of 2,494 ticks was collected by means of drag sampling during one year of survey (1993). Among the species identified, Ixodes ricinus (Linnaeus, 1758) (95.9%) was the most abundant, followed by Haemaphysalis inermis (Birula, 1895) (2.2%), Rhipicephalus bursa (Canestrini e Fanzago, 1877) (1.8%), Dermacentor marginatus Sulzer, 1776) (0.1%). I. ricinus was present during the whole year, showing a diphasic trend, with peaks in January-April (95-173 specimens per drag) and in November-December (29-126); H. inermis was recovered only during the cold season (January-April and in December) with the highest density in February (7); Rh. bursa was collected from May to October showing maximum occurrence in July (10). The presence of I. ricinus and H. inermis, proven vectors of Borrelia burgdorferi (Lyme disease) and TBE virus, is discussed in relation to the possible exposure risk for park visitors and operators.