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1.
Appl Opt ; 60(35): 10984-10987, 2021 Dec 10.
Article in English | MEDLINE | ID: mdl-35200861

ABSTRACT

We report an experimental study of long-wave infrared difference frequency generation based on BaGa4Se7 crystal. The sources of two input wavelengths were the fundamental output of a Nd:YAG laser and its second-harmonic pumped ∼1.2µmKTiOPO4 optical parametric oscillator. A wide tuning range of 7.9-17.5 µm (>1.14 octave) was achieved, which reached the upper limit of the BaGa4Se7 transparency region. The spectra and pulse widths, input-output relationship, beam profile, wavelength tolerance, and angular acceptance of the phase-matching were characterized in detail. This presented coherent source can potentially be applied in multiple gas analyses and spectral imaging.

2.
J Gene Med ; 22(2): e3147, 2020 02.
Article in English | MEDLINE | ID: mdl-31758726

ABSTRACT

BACKGROUND: Increasing evidence shows that gastrulation brain homeobox 2 (GBX2) is involved in multiple cancers. However, whether GBX2 has an effect on the lung adenocarcinoma remains unclear. In the present study, we investigated the functions of GBX2 on lung adenocarcinoma and explored the underlying mechanism. METHODS: Public data were obtained from the TCGA (https://cancergenome.nih.gov) and Oncomine (http://www.oncomine.org) databases. GBX2 expression and its prognostic value were analyzed by bioinformatics methods. Relative mRNA and protein expression levels of GBX2 in lung adenocarcinoma cell lines were evaluated via a quantitative reverse transcriptase polymerase chain reaction and western blotting. Lung adenocarcinoma cell lines LTEP-a-2 and A549, respectively, were selected for gain and loss function of GBX2 assays. Cell viability was detected by CCK8 and clone formation experiments. Cell invasion and migration were assessed by Transwell assays. The effect of GBX2 on the AKT/extracellular signal regulated kinase (ERK) pathway was tested by western blotting. RESULTS: Compared to adjacent tissues, GBX2 expression was up-regulated in lung adenocarcinoma tissues. High expression of GBX2 led to a poor survival and could be seen as an independent predictor for lung adenocarcinoma patients. Furthermore, down-regulation of GBX2 notably restrained the viability, invasion and migration abilities of A549 cells, whereas up-regulation of GBX2 in LTEP-a-2 cells presented the opposite outcomes. Furthermore, western blot indicated that down-regulation of GBX2 decreases the protein levels of phosphorylated (p)-AKT and p-ERK in A549 cells, whereas up-regulation of GBX2 shows the opposite effects in LTEP-a-2 cells. CONCLUSIONS: The results of present study indicate that GBX2 acts a cancer-promoting role to accelerate cell proliferation, invasion and migration partly by modulation of the AKT/ERK pathway in lung adenocarcinoma.


Subject(s)
Adenocarcinoma of Lung/pathology , Homeodomain Proteins/metabolism , Lung Neoplasms/pathology , MAP Kinase Signaling System , A549 Cells , Adenocarcinoma of Lung/genetics , Cell Line, Tumor , Cell Movement , Cell Proliferation , Cell Survival , Extracellular Signal-Regulated MAP Kinases/metabolism , Female , Gene Expression Regulation, Neoplastic , Gene Knockdown Techniques , Homeodomain Proteins/genetics , Humans , Lung Neoplasms/genetics , Male , Middle Aged , Neoplasm Invasiveness , Prognosis , Proto-Oncogene Proteins c-akt/metabolism , Up-Regulation
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