ABSTRACT
It was studied for community structure of microorganisms in the phosphorus removal processes under the circulating situation, and analyzed for microorganism's structure and behavior characteristics by the molecular biology technique with direct obtaining of DNA from samples of activated sludge, and by nested PCR and DGGE. It was also determined community structure of microorganisms. It was analyzed structures of Proteobacteria and Acidobacterium by 16S rDNA V3 area gene fragments sequences in activated sludge. By comparing gene sequences in the National Center of Biological Information (NCBI), were determined the kinds of part of microorganisms. Analyzing the low of changes of preponderant bacteria in anaerobic/aerobic and anaerobic/anoxic conditions takes to know, that under the stable situation of phosphorus removing, the system of microorganism's structure can kept mostly constant. Minority races that have changed in amount or kind has something to do with the variation of oxygen level in the system, but structure totally can adapt the environmental conditions of the processes, while it placed in dynamic varieties.
Subject(s)
Actinobacteria/metabolism , Phosphorus/metabolism , Proteobacteria/metabolism , Waste Disposal, Fluid/methods , Actinobacteria/genetics , Actinobacteria/isolation & purification , Aerobiosis , Ecosystem , Electrophoresis, Gel, Pulsed-Field/methods , Phosphorus/isolation & purification , Polymerase Chain Reaction/methods , Proteobacteria/genetics , Proteobacteria/isolation & purification , RNA, Ribosomal, 16S/geneticsABSTRACT
It is reported that without cultivation, DNA could be directly extracted from environmental samples with molecular biological methods, such as polymerase chain reaction (PCR) and denaturting gradient gel electrophoresis (DGGE). To analyze the community diversity of activated sludge and bio-film in the municipal sewage, work was done to directly extrude crude DNA from activated sludge and bio-film samples, separate and amplify 16S rDNA by PCR and sequence it with DGGE. The results show the significant microbe community difference between cultivated and uncultivated activated sludge. Further research on the community diversity of two different sewage treatment processes was done and initial discussion on the microbial distribution in the same reactor and microbial structure in different experimental conditions was carried out. The sequences of several 16S rDNA DGGE fragments were determined and some possible bacteria were confirmed in comparision in GeneBank (NCBI). The results show that the PCR-DGGE technology combined with sequences determination is a feasible and efficient method for microorganism analysis in environmental sample.
