Medical withdrawals in elite tennis in reference to playing standards, court surfaces and genders.

OBJECTIVES: To examine the relationships between medical withdrawals, playing standards, court surfaces and genders in tennis players participating in all elite tours. DESIGN: Descriptive epidemiology study. METHODS: Medical withdrawals of men and women tennis players from Association of Tennis Professionals, Women Tennis Association, Challengers and International Tennis Federation Futures tours' matches have been identified considering the court surfaces (fast vs. slow). Proportion comparison and the binomial regression model were used to determine the influence of playing standards, court surfaces and genders on tennis players' likelihoods to withdraw. RESULTS: A higher proportion of withdrawals was found for men in Challengers and Futures vs. Association of Tennis Professionals (4.8 %, 5.9 % vs 3.4 %; p < 0.001), but without difference between court surfaces (0.1 %, p > 0.05) whatever the playing standards. Women reported higher proportion of medical withdrawals sustained on slow surfaces (0.4 %, p < 0.001), but without different withdrawal rates between playing standards (3.9 %, p > 0.05). After adjustment, the odds of medical withdrawals were higher for Challengers (1.18, p < 0.001) and Futures (1.34, p < 0.001), with a higher likelihood to withdraw (1.04, p < 0.001) when playing on slow surfaces and with a gender-dependent effect indicating higher odds (1.29, p < 0.001) to withdraw for medical reasons in men in reference to women. CONCLUSIONS: These findings demonstrated a gender-dependent effect on medical withdrawals from an elite tennis tournament with higher likelihood for men participating in Challengers/Futures tours and for women playing on slow surfaces.

Winding Vector: How to Annihilate Two Dirac Points with the Same Charge.

The merging or emergence of a pair of Dirac points may be classified according to whether the winding numbers which characterize them are opposite (+- scenario) or identical (++ scenario). From the touching point between two parabolic bands (one of them can be flat), two Dirac points with the same winding number emerge under appropriate distortion (interaction, etc.), following the ++ scenario. Under further distortion, these Dirac points merge following the +- scenario, that is corresponding to opposite winding numbers. This apparent contradiction is solved by the fact that the winding number is actually defined around a unit vector on the Bloch sphere and that this vector rotates during the motion of the Dirac points. This is shown here within the simplest two-band lattice model (Mielke) exhibiting a flat band. We argue on several examples that the evolution between the two scenarios is general.

Mass and chirality inversion of a Dirac cone pair in Stückelberg interferometry.

We show that a Stückelberg interferometer made of two massive Dirac cones can reveal information on band eigenstates such as the chirality and mass sign of the cones. For a given spectrum with two gapped cones, we propose several low-energy Hamiltonians differing by their eigenstates properties. The corresponding interband transition probability is affected by such differences in its interference fringes being shifted by a new phase of geometrical origin. This phase can be a useful bulk probe for topological band structures realized with artificial crystals.

Bloch-Zener oscillations across a merging transition of Dirac points.

Bloch oscillations are a powerful tool to investigate spectra with Dirac points. By varying band parameters, Dirac points can be manipulated and merged at a topological transition toward a gapped phase. Under a constant force, a Fermi sea initially in the lower band performs Bloch oscillations and may Zener tunnel to the upper band mostly at the location of the Dirac points. The tunneling probability is computed from the low-energy universal Hamiltonian describing the vicinity of the merging. The agreement with a recent experiment on cold atoms in an optical lattice is very good.

Spontaneous parity breaking of graphene in the Quantum Hall regime.

We propose that the inversion symmetry of the graphene honeycomb lattice is spontaneously broken via a magnetic-field-dependent Peierls distortion. This leads to valley splitting of the n=0 Landau level but not of the other Landau levels. Compared to Quantum Hall valley ferromagnetism recently discussed in the literature, lattice distortion provides an alternative explanation to all of the currently observed Quantum Hall plateaus in graphene.

New model potentials for sulfur-copper(I) and sulfur-mercury(II) interactions in proteins: from ab initio to molecular dynamics.

We have developed new force field and parameters for copper(I) and mercury(II) to be used in molecular dynamics simulations of metalloproteins. Parameters have been derived from fitting of ab initio interaction potentials calculated at the MP2 level of theory, and results compared to experimental data when available. Nonbonded parameters for the metals have been calculated from ab initio interaction potentials with TIP3P water. Due to high charge transfer between Cu(I) or Hg(II) and their ligands, the model is restricted to a linear coordination of the metal bonded to two sulfur atoms. The experimentally observed asymmetric distribution of metal ligand bond lengths (r) is accounted for by the addition of an anharmonic (r3) term in the potential. Finally, the new parameters and potential, introduced into the CHARMM force field, are tested in short molecular dynamics simulations of two metal thiolates fragments in water. (Brooks BR et al. J Comput Chem 1983, 4, 1987.1).

Inhibition of DNA helicases with DNA-competitive inhibitors.

Helicases form an attractive protein family for drug discovery because they are involved in various human diseases. In this report, we show that it is possible to inhibit both the ATPase and the helicase activities of a DNA helicase with dibenzothiepins that bind at its nucleic acid binding site. These results suggest a drug discovery strategy to inhibit DNA helicases.

Molecular dynamics study of the metallochaperone Hah1 in its apo and Cu(I)-loaded states: role of the conserved residue M10.

Molecular dynamics simulations were performed on both apo and copper forms of the human copper chaperone, Hah1. Wild-type Hah1 and a methionine (M10) to serine mutant were investigated. We have evidenced the central role of residue M10 in stabilizing the hydrophobic core of Hah1 as well as the internal structure of the metal-binding site. When copper(I) is bound, the mobility of Hah1 is reduced whereas mutation of M10 implies a drastic increase of the mobility of apoHah1, stressing the importance of this highly conserved hydrophobic residue for copper sequestration by the apoprotein.

Purkinje-cell degeneration in prion protein-deficient mice is associated with a cerebellum-specific Doppel protein species signature.

PrP(c) (cellular prion protein) and Doppel are antagonizing proteins, respectively neuroprotective and neurotoxic. Evidence for Doppel neurotoxicity came from PrP(c)-deficient (Prnp(0/0)) mouse lines developing late onset Purkinje-cell degeneration caused by Doppel overexpression in brain. To address the molecular underpinnings of this cell-type specificity, we generated Doppel N-terminal-specific antibodies and started to examine the spatio-temporal expression of Doppel protein species in Ngsk Prnp(0/0) brain. Although Doppel overexpression is ubiquitous, Western analyses of normal and deglycosylated protein extracts revealed cerebellar patterns distinct from the rest of the brain, supporting the idea that neurotoxicity might be linked to a particular Doppel species pattern. Furthermore, our newly raised antibodies allowed the first Doppel immunohistochemical analyses in brain, showing a distribution in Prnp(0/0) cerebellum similar to PrP(c) in wild type.

Different chromogranin immunoreactivity between prion and a-beta amyloid plaque.

Brain lesions in Creutzfeldt-Jakob disease (CJD) include spongiform change, neuronal loss, amyloid plaques, astrogliosis and microglial activation. Microglia are thought to play a key role in prion-induced neurodegeneration. However, the intermediate molecules supporting relationships between neurons and microglia are still unknown. Chromogranins (Cg) are soluble glycophosphoproteins that can activate microglial cells leading to a neurotoxic phenotype. The immunoreactive patterns of CgA and CgB were investigated in CJD and compared to those observed in Alzheimer's disease. We found that CgB, but not CgA, immunoreactivity was selectively associated with prion protein deposits, whereas CgA was only seen in Abeta plaques. This suggests a specific influence of the constitutive amyloid protein on chromogranin secretion and a role of CgB in the CJD neurodegenerative process.

Study of the cytotoxic effect of a peptidic inhibitor of the p53-hdm2 interaction in tumor cells.

Inhibitors of the p53-hdm2 interaction are attractive molecules for stimulating the p53 pathway in tumors. In this report, an inhibitor of the p53-hdm2 interaction, the AP peptide, is used to activate p53 in tumor cells expressing various levels of hdm2 protein. It induces apoptosis only in cells expressing high endogenous levels of hdm2 protein. The absence of apoptosis in tumor cells with low hdm2 levels is due not to alterations in the p53-dependent apoptotic pathway but to a different regulation of this pathway. The peptide is also less toxic for non-tumor cells than for tumor cells overexpressing the hdm2 protein.