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Mol Gen Mikrobiol Virusol ; (2): 16-9, 1991 Feb.
Article in Russian | MEDLINE | ID: mdl-2030701

ABSTRACT

Chicken embryonated eggs were coinfected with influenza A/FPV/Rostock and A/FPV/Weybridge strains. 25 plaque isolates were obtained from the mixed yield and their genetic content was analysed by polyacrylamide gel electrophoresis of H3-uridine-labelled vRNA in a modified gel system. At least 18 clones out of 25 plaque isolates proved to be reassortants; however, only one among them contained the homologous RNA-segments belonging to both parents. The results are in agreement with the concept of an ordered recruitment of vRNA-segments into virions.


Subject(s)
Genes, Viral , Influenza A virus/genetics , RNA, Viral/genetics , Animals , Chick Embryo , Clone Cells , Electrophoresis, Polyacrylamide Gel , Virion
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