ABSTRACT
In this study, we applied protamine, which is an antimicrobial peptide, to oral healthcare in combination with conventional antimicrobial agents. First, we explored the antimicrobial activity of protamine, with or without other antimicrobial agents, against Streptococcus mutans (S. mutans). Co-treatment with protamine and 3-methyl-4-isopropylphenol (IPMP) decreased the viability of S. mutans synergistically within 10 min. Interestingly, sodium fluoride (NaF) did not exhibit synergistic activity with protamine. Next, S. mutans and Streptococcus gordonii (S. gordonii) were co-treated with protamine and IPMP for 5 min to simulate tooth brushing. As a result, this co-treatment killed S. mutans faster than S. gordonii. Therefore, co-treatment with protamine and IPMP could be incorporated into oral healthcare products to prevent dental caries.
Subject(s)
Anti-Bacterial Agents/pharmacology , Protamines/pharmacology , Streptococcus gordonii/drug effects , Streptococcus mutans/drug effects , Dental Caries/drug therapy , Dental Caries/microbiology , Drug Synergism , Humans , Microbial Sensitivity Tests , Sodium Fluoride/pharmacology , Streptococcal Infections/prevention & controlABSTRACT
Protamine is an antimicrobial peptide extracted from fish. In this study, we loaded protamine onto dicalcium phosphate anhydride (DCPA), a dental material. Protamine was loaded by stirring DCPA into a protamine solution. To explore the antimicrobial activity of the materials, we cultivated Streptococcus mutans on fabricated discs for 24 h. When S. mutans was cultivated on the discs under no sucrose conditions, the loaded protamine was not released, and the ratio of dead bacteria increased on the surface of P (125) DCPA (half of the saturated level of protamine (125 ppm protamine) was loaded). Aside from P (500) DCPA (saturated level of protamine was loaded), some protamine was released, and the number of planktonic bacteria in the supernatant decreased. Using medium containing 1% sucrose, the release of protamine was promoted from P (125) DCPA due to lowered pH. However, lowering of the pH decreased the antimicrobial activity of protamine. On the other hand, P (500) DCPA released protamine before the pH was lowered, and biofilm formation was inhibited. The loaded protamine expressed antimicrobial activity, both on the surface of the materials and in the surrounding environment. The interaction of loaded protamine with calcium phosphates could promote the application of protamine in the dental field.
