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1.
Biochem Biophys Res Commun ; 309(1): 18-25, 2003 Sep 12.
Article in English | MEDLINE | ID: mdl-12943657

ABSTRACT

Useful biomarkers are needed for early detection of cancers. To demonstrate the potential diagnostic usefulness of a new proteomic technology, we performed Expression Difference Mapping analysis on 39 cancer cell lines from 9 different tissues using ProteinChip technology. A protein biomarker candidate of 12kDa was found in colon cancer cells. We then optimized the purification conditions for this biomarker by utilizing Retentate Chromatography mass spectrometry (RC-MS). The optimized purification conditions developed "on-chip" were directly transferred to conventional chromatography to purify the biomarker, which was identified as prothymosin-alpha by ProteinChip time-of-flight mass spectrometry (TOF MS) and ProteinChip-Tandem MS systems. The relative expression level of prothymosin-alpha between colon cancer cells and normal colon mucosal cells was evaluated on the same ProteinChip platform. Prothymosin-alpha expression in colon cancer cells was clearly higher than in normal colon cells. These results indicate that prothymosin-alpha could be a potential biomarker for colon cancer, and that the ProteinChip platform could perform the whole process of biomarker discovery from screening to evaluation of the identified marker.


Subject(s)
Biomarkers, Tumor , Protein Array Analysis/methods , Thymosin/analogs & derivatives , Colon/cytology , Colon/metabolism , Dose-Response Relationship, Drug , Electrophoresis, Polyacrylamide Gel , Humans , Mass Spectrometry , Protein Precursors/biosynthesis , Thymosin/biosynthesis , Trypsin/pharmacology , Tumor Cells, Cultured
2.
J Proteome Res ; 1(6): 495-9, 2002.
Article in English | MEDLINE | ID: mdl-12645617

ABSTRACT

We compared protein profiles of the synovial fluid of patients with rheumatoid arthritis and osteoarthritis by using surface-enhanced laser desorption/ionization mass spectrometry technology. With this approach, we identified a protein expressed specifically in the synovial fluid of the patients with rheumatoid arthritis. During the investigation, we found several reproducible and discriminatory biomarker candidates for distinction between rheumatoid arthritis and osteoarthritis. Among these candidates, a 10 850 Da protein peak was the clearest example of a single signal found specifically in the rheumatoid arthritis samples. This candidate was purified using a size-exclusion spin column followed by gel electrophoresis and subsequently identified by peptide mapping and post-source decay (PSD) analysis. The results clearly indicate that the protein is myeloid-related protein 8, which was verified by the enzyme immunoassay. It is known that the myeloid-related protein 8 level in serum and synovial fluid is related to disease activity in juvenile rheumatoid arthritis. The results suggest that the ProteinChip platform is useful to detect and identify protein biomarkers expressed specifically in diseases or in some stage of diseases.


Subject(s)
Arthritis, Rheumatoid/diagnosis , Arthritis, Rheumatoid/metabolism , Biomarkers , Protein Array Analysis , Synovial Fluid/metabolism , Calgranulin A/metabolism , Calgranulin B/metabolism , Humans , Mass Spectrometry
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