ABSTRACT
We describe a patient with scleroderma en coup de sabre and central nervous system symptoms. She presented with a linear scleroderma on her left paramedian forehead and scalp and suffered from headaches and dizziness. Axial T2-weighted MRI showed a 1 cm, high intense signal abnormality in the medial aspect of the left frontal lobe.
Subject(s)
Dizziness/etiology , Headache/etiology , Scleroderma, Localized/complications , Scleroderma, Localized/diagnosis , Adolescent , Central Nervous System Diseases/diagnosis , Central Nervous System Diseases/etiology , Dizziness/diagnosis , Female , Headache/diagnosis , Humans , Magnetic Resonance ImagingABSTRACT
We describe three cases of polyarteritis nodosa cutanea (PNC) showing necrotizing arteritis and only cutaneous lesions without systemic symptoms or visceral involvement for eleven, six, and three years after the onset of the disease. Since it was first described, there has been continuous controversy as to whether PNC progresses to systemic PN. Some cases have been described which had begun with a cutaneous lesion and progressed to the systemic form 19 and 18 years after the onset of the disease, so we believe that long term follow-up of this disease is essential.
Subject(s)
Polyarteritis Nodosa/pathology , Skin Diseases, Vascular/pathology , Adrenal Cortex Hormones/administration & dosage , Adult , Aged , Angiography , Biopsy, Needle , Chronic Disease , Female , Follow-Up Studies , Humans , Male , Polyarteritis Nodosa/diagnosis , Polyarteritis Nodosa/drug therapy , Skin Diseases, Vascular/diagnosis , Skin Diseases, Vascular/drug therapyABSTRACT
We introduced a novel convenient method to prepare chromosome-less mini-cells from cell mixture of Escherichia coli P678-54 by using penicillin. The mini-cells prepared by this method could successfully transferred their harboring conjugative plasmids into eukaryotic yeast cells by trans-kingdom conjugation.
Subject(s)
Conjugation, Genetic , Escherichia coli/genetics , Saccharomyces cerevisiae/genetics , Chromosomes, Bacterial/genetics , Genetic Techniques , Microscopy, Fluorescence , Penicillins , Plasmids/geneticsABSTRACT
Tobacco BY2 cells were withered away by the physical contact with intestinal bacterium Escherichia coli. In contrast, plant pathogenic bacterium Agrobacterium tumefaciens with/without tumor inducing Ti plasmid was not so toxic as E. coli. Mini-cells of E. coli decreased their toxicity.
Subject(s)
Agrobacterium tumefaciens/pathogenicity , Escherichia coli/pathogenicity , Nicotiana/microbiology , Agrobacterium tumefaciens/genetics , Cell Line , Gene Transfer Techniques , Gene Transfer, Horizontal , Plant Diseases/microbiology , Plasmids/genetics , VirulenceABSTRACT
L-band ESR spectrometry has been developed for detecting free radicals or paramagnetic metal ions in intact animals or biological samples which contain much water. Several studies by L-band ESR on free radicals have been reported, but few on paramagnetic elements such as copper(II) or iron(III). We measured L-band ESR spectra of Cu(II) for the first time to investigate the coordination structure around the metal ion in aqueous solution. In X- and L-band ESR, both spectral patterns and parameters have been found to reflect the coordination structure of Cu(II)-polyamine complexes depending on the structures such as square-planar, axially coordinate square-planar, tetrahedral and distorted square-planar (Cu(II) ion is displaced from CuN4 plane). In addition, in L-band ESR, the coordination structures of Cu(II) complexes relate with spectral pattern, ESR parameter (LA-value), stability constants and halfwave potentials in terms of the electron density on Cu(II). Thus, L-band ESR spectrometry was indicated to be useful to know the coordination structure of Cu(II) proteins or enzymes in aqueous solution.
