1.
J Am Chem Soc
; 127(1): 68-9, 2005 Jan 12.
Article
in English
| MEDLINE
| ID: mdl-15631452
ABSTRACT
Fluorometric detection of O2-* is performed based on desulfonylation of 3 to the corresponding fluoresceins 4 through nucleophilic substitution, and this fluorescing process is quite specific toward O2-* over H2O2, t-BuOOH, NaOCl, 1O2, HO*, NO*, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe 3 with X = Y = F, which is useful to detect O2-* released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.
Subject(s)
Fluoresceins/chemistry , Fluorescent Dyes/chemistry , Superoxides/chemistry , Benzenesulfonates/chemistry , Cytochrome P-450 Enzyme System/chemistry , Drug Design , Fluoresceins/chemical synthesis , Fluorescent Dyes/chemical synthesis , Fluorometry , Glutathione/chemistry , Hydrogen Peroxide/chemistry , Hypoxanthine/chemistry , NADP/chemistry , Oxidation-Reduction , Sulfonic Acids/chemistry , Superoxide Dismutase/chemistry , Superoxides/analysis , Xanthine Oxidase/chemistry
2.
Angew Chem Int Ed Engl
; 43(18): 2389-91, 2004 Apr 26.
Article
in English
| MEDLINE
| ID: mdl-15114569